垂穗披碱草MADS-box基因WM8克隆及分析

草业学报 ›› 2012, Vol. 21 ›› Issue (4): 141-150.

垂穗披碱草MADS-box基因WM8克隆及分析

张妙青,张吉宇,刘志鹏,王彦荣^*,张磊

草地农业生态系统国家重点实验室兰州大学草地农业科技学院,甘肃兰州 730020

收稿日期:2011-04-21 出版日期:2012-04-25 发布日期:2012-08-20
通讯作者: E-mail:yrwang@lzu.edu.cn
作者简介:张妙青(1984-),女,陕西岐山人,在读硕士。E-mail:plantseed@yeah.net
基金资助:
国家“973”课题(编号:2007CB108904),兰州大学中央高校基本科研费专项基金(编号:lzujbky-2009-108,lzujbky-2009-4),“十一五”国家科技支撑计划子课题(编号:2008BADB3B03-4)资助。

Cloning and analysis of the MADS-box gene WM8 of Elymus nutans

ZHANG Miao-qing, ZHANG Ji-yu, LIU Zhi-peng, WANG Yan-rong, ZHANG Lei

State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

Received:2011-04-21 Online:2012-04-25 Published:2012-08-20

摘要/Abstract

摘要： 通过同源克隆从垂穗披碱草中获得了MADS-box基因家族WM8基因的全长cDNA序列,命名为 EnWM8(Genbank登录号为 JF683846)。序列分析表明,该cDNA全长1 196 bp,开放阅读框共编码275个氨基酸,具有典型的MADS-box结构域以及半保守的K区,是MIKC型MADS-box基因,分析认为其与花发育和果实成熟等有关。EnWM8与小麦的WM8、AGL29和fruitful-like基因氨基酸的推导序列的相似性分别高达95.62%,96.72%和96.28%。采用DNAMAN 软件进行系统进化树分析显示,EnWM8基因与小麦的TaWM8进化关系最近,与拟南芥的AtWM8、AtAGL29进化关系较远。垂穗披碱草WM8蛋白分子量为13 726.7 Da,理论等电点为9.14,为亲水性的碱性蛋白,无跨膜结构域,定位于细胞核中,其空间结构主要由α-螺旋、无规则卷曲和延伸链构成。研究结果可为进一步从分子水平探明垂穗披碱草花发育、种子成熟等机制提供参考。

Abstract: The full length MADS-box gene EnWM8 was isolated from Elymus nutans by homology cloning, and was submitted to GenBank (accession number JF683846). Analyses of the cDNA sequence showed that EnWM8 was 1 196 bp and encoded 275 amino acids, typical of the MIKC-type MADS-box genes with a typical MADS-box domain and K semi-conservative district. It is speculated that it has some connection with flower development, fruit ripening etc. The deduced amino acid sequence showed that there were 95.62%, 96.72%, 96.28% similarities between the gene and Triticum aestivum genes WM8, AGL29, and fruitful-like. The phylogenetic tree deduced by DNAMAN software indicated that EnWM8 had a close evolutionary relationship with the TaWM8 of T. aestivum, and was distantly related to the AtWM8 and AtAGL29 of Arabidopsis. The WM8 protein of E. nutans is a hydrophilic and basic protein with a molecular weight of 13 726.7 Da: the theoretical isoelectric point is 9.14. It has no membrane structure and is mainly located in the nucleus. There are many α-helix, random coil and extended strand structures. These results are a basis for further research on flower development, seed maturation and other mechanisms of E. nutans at the molecular level.

中图分类号:

张妙青,张吉宇,刘志鹏,王彦荣,张磊. 垂穗披碱草MADS-box基因WM8克隆及分析[J]. 草业学报, 2012, 21(4): 141-150.

ZHANG Miao-qing, ZHANG Ji-yu, LIU Zhi-peng, WANG Yan-rong, ZHANG Lei. Cloning and analysis of the MADS-box gene WM8 of Elymus nutans [J]. Acta Prataculturae Sinica, 2012, 21(4): 141-150.

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