添加酶和乳酸菌制剂对西藏苇状羊茅和箭筈豌豆混合青贮发酵品质的影响

草业学报 ›› 2012, Vol. 21 ›› Issue (4): 186-191.

添加酶和乳酸菌制剂对西藏苇状羊茅和箭筈豌豆混合青贮发酵品质的影响

王奇¹,余成群²,李志华¹,赵庆杰¹,下条雅敬³,邵涛^1*

1.南京农业大学动物科学技术学院饲草调制加工与贮藏研究所,江苏南京 210095;
2.中国科学院地理科学与资源研究所,北京100101;
3.九州大学生物资源与环境学部动物饲料生产与利用研究室,日本福冈 812-8581

收稿日期:2011-08-17 出版日期:2012-04-25 发布日期:2012-08-20
通讯作者: E-mail:taoshaolan@yahoo.com.cn
作者简介:王奇(1989-),男,山东成武人,在读硕士。E-mail:wang_qi57@126.com
基金资助:
西藏主要农作物秸秆与栽培牧草混合青贮关键技术研究(XZ20093ZD)和国家科技支撑计划“藏北退化草地综合整治技术与示范”(2010BAE00739－03)资助。

Effect of adding coenzyme and lactic acid bacteria on fermentation quality of mixed silages of tall fescue (Festuca arundinacea) and common vetch (Vicia sativa) in tibet

WANG Qi¹, YU Cheng-qun², LI Zhi-hua¹, ZHAO Qing-jie¹, Masataka Shimojo³, SHAO Tao¹

1.Institute of Ensiling and Processing of Grass, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
2.Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;
3.Laboratory of Animal Feed Science, Division of Animal Science, Department of Animal and Marine Bioresource Sciences, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan

Received:2011-08-17 Online:2012-04-25 Published:2012-08-20

摘要/Abstract

摘要： 为评价添加酶、乳酸菌制剂和酶+乳酸菌制剂对苇状羊茅与箭筈豌豆(7∶3)混合青贮发酵品质的影响,试验设对照组(C)、酶制剂(E)、乳酸菌制剂(LAB)和酶+乳酸菌制剂(E+LAB)4个处理,3个重复,青贮后第7,24,60天开窖取样,测定青贮饲料发酵品质。结果表明,与对照组相比,添加酶或乳酸菌制剂能有效地改善混合青贮发酵品质,组合添加进一步提高了乳酸含量和降低了pH值。与对照组和单独添加乳酸菌组相比,在整个青贮过程中无论是酶单独添加还是组合添加都显示较高的乙酸和总挥发性脂肪酸含量,最高的水溶性碳水化合物含量和最低的氨态氮/总氮。这说明酶和乳酸菌制剂组合添加对改善青贮发酵品质有叠加效应。综上所述,酶和乳酸菌制剂组合添加能更好的改善苇状羊茅和箭筈豌豆混合青贮的发酵品质。

Abstract: The aim of this study is to evaluate the effects of adding coenzyme, LAB and their combinations on the fermentation qualities and residual water soluble carbohydrates of mixed silages of common vetch and tall fescue during ensiling. The treatments were as follows: control (C),coenzyme (E), LAB, coenzyme+LAB(E+LAB), these silos were opened on 7, 24 and 60 days after ensiling and the fermentation quality was analyzed. Compared to the control silage, adding of E and LAB were efficient in improving the fermentation quality of mixed silages, and the combination addition further increased the LA content and decreased the pH value. The E combination treatments including the E alone showed higher acetic acid and total volatile fatty acids contents as compared with the control and LAB treated silages, the propionic and butyric acid were hardly found in all silages during ensiling. The combination treated silages showed highest water soluble carbohydrate content and lowest ammonia nitrogen/total nitrogen through the whole ensiling. These indicated that the E and LAB were of synergistic effects on the fermentation quality. From the above results it was suggested that the combination addition of coenzyme and LAB is better for improving the fermentation quality of mixed silages of common vetch and tall fescue in this experiment.

中图分类号:

S816.6

王奇,余成群,李志华,赵庆杰,下条雅敬,邵涛. 添加酶和乳酸菌制剂对西藏苇状羊茅和箭筈豌豆混合青贮发酵品质的影响[J]. 草业学报, 2012, 21(4): 186-191.

WANG Qi, YU Cheng-qun, LI Zhi-hua, ZHAO Qing-jie, Masataka Shimojo, SHAO Tao. Effect of adding coenzyme and lactic acid bacteria on fermentation quality of mixed silages of tall fescue (Festuca arundinacea) and common vetch (Vicia sativa) in tibet[J]. Acta Prataculturae Sinica, 2012, 21(4): 186-191.

参考文献

[1] 玉柱, 贾玉山. 牧草饲料加工与贮藏[M]. 北京: 中国农业大学出版社, 2010.
[2] McDonald P, Henderson A R, Heron S J E. The Biochemistry of Silage (2th ed)[M]. Aberystwyth: Cambrian Printers Ltd, 1991.
[3] Xing L, Chen L J, Han L J. The effect of an inoculant and enzymes on fermentation and nutritive value of sorghum straw silages[J]. Bioresource Technology, 2009, 100(1): 488-491.
[4] Filya I. The effect of lactobacillus buchneri and lactobacillus plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corn and sorghum silages[J]. Journal of Dairy Science, 2003, 86(11): 3575-3581.
[5] 吕文龙, 刁其玉, 闫贵龙. 布氏乳杆菌对青玉米秸青贮发酵品质和有氧稳定性的影响[J]. 草业学报, 2011, 20(4): 143-148.
[6] Eun J S, Beauchemin K A. Relationship between enzymic activities and in vitro degradation of alfalfa hay and corn silage[J]. Animal Feed Science and Technology, 2008, 145(1-4): 53-67.
[7] 贾燕霞, 玉柱, 邵涛, 等. 添加酶制剂对象草青贮发酵品质的影响[J]. 草地学报, 2009, 17: 121-124.
[8] Chen J, Stokes M R, Wallace C R. Effects of enzyme-inoculant systems on preservation and nutritive value of haycrop and corn silages[J]. Journal of Dairy Science, 1994, 77(2): 501-512.
[9] Barker S B, Summerson W H. The colorimetric determination of lactic acid in biological material[J]. Journal of Biology Chemistry, 1941, 138: 535-554.
[10] Owens V N, Albrecht K A, Muck R E. Protein degradation and fermentation characteristics of unwilted red clover and alfalfa silage harvested at various times during the day[J]. Grass and Forage Science, 2002, 57(4): 329-341.
[11] Weather M W. Phenol-hypochlorite reaction for determinations of ammonia[J]. Annual of Chemistry, 1967, 39: 971-974.
[12] AOAC. Official methods of Analysis (14th ed)[M]. Virginia, USA: Association of Official and Analytical Chemists Arlington, 1984.
[13] Shao T, Zhang Z X, Shimojo M, et al. Comparison of fermentation characteristics of italian ryegrass(Lolium multiflorum Lam.) and guineagrass (Panicum maximum Jacq.) during the early stage of ensiling[J]. Asian-Australasian Journal of Animal Sciences, 2005, 18(12): 1727-1734.
[14] SAS. SAS/STAT User’s Guide: Version 6. 4th ed. ed.[M]. Cary, North Carolina: SAS Institute Inc, 1984.
[15] 蒋慧, 张玲, 马春晖, 等. 枯黄期骆驼刺与稻草混贮对青贮饲料品质的影响[J]. 草业学报, 2011, 20(2): 109-116.
[16] 王林, 孙启忠, 张慧杰. 苜蓿与玉米混贮质量研究[J]. 草业学报, 2011, 20(4): 202-209.
[17] Colombatto D, Mould F L, Bhat M K, et al. In vitro evaluation of fibrolytic enzymes as additives for maize (Zea mays L.) silage effects of ensiling temperature, enzyme source and addition level[J]. Animal Feed Science and Technology, 2004, 111(1-4): 111-128.
[18] Shao T, Zhang L, Shimojo M, et al. Fermentation quality of italian ryegrass (Lolium multiflorum Lam.) silages treated with encapsulated-glucose, glucose, sorbic acid and pre-fermented juices[J]. Asian-Australasian Journal of Animal Sciences, 2007, 20: 1699-1704.
[19] Seale D R. Bacteria and Enzymes as Products to Improve Silage Preservation[M]. Developments in Silage Chalcombe Publ. marlow. pp. 1987: 147-171.
[20] Nilsson G, Nilsson P E. Silage studies. IV. The microflora on the surface of some fodder plants at different stages of maturity[J]. Archives of Microbiology, 1956, 24(4): 412-422.
[21] Shao T, Ohba N, Shimojo M, et al. Effects of addition of glucose and sorbic acid on the fermentationi quality of guineagrass(Panicum maximum jacq.) silages[J]. Journal of the Faculty of Agriculture, 2003, 47(2): 351-358.