祁连山不同海拔梯度山生柳遗传多样性的SSR分析

草业学报 ›› 2012, Vol. 21 ›› Issue (5): 114-121.

祁连山不同海拔梯度山生柳遗传多样性的SSR分析

郭敏,马彦军,李毅^*

甘肃农业大学林学院,甘肃兰州 730070

收稿日期:2011-07-21 出版日期:2012-05-25 发布日期:2012-10-20
通讯作者: E-mail:liyi@gsau.edu.cn
作者简介:郭敏(1987-),女,甘肃天水人,在读硕士。E-mail:guomin.melyssa@163.com
基金资助:
教育部高等学校博士点基金(20096202110002)资助。

Analysis of genetic diversity in Salix oritrepha from different altitudes in Gansu Qilian Mountains using SSR markers

GUO Min, MA Yan-jun, LI Yi

Forestry College of Gansu Agricultural University, Lanzhou 730070, China

Received:2011-07-21 Online:2012-05-25 Published:2012-10-20

摘要/Abstract

摘要： 本实验采用SSR分子标记技术,对甘肃祁连山脉9个海拔梯度共180个单株的山生柳遗传多样性和遗传结构进行了研究。利用杨属植物的47对SSR引物对山生柳进行了通用性分析,筛选出15对重复性高、条带清晰的引物,结果显示,15条引物共检测到211条谱带,其中多态性条带有176条,平均每个引物检测到11个等位变异,多态位点比率(P)为85%。应用分析软件Popgen 32进行分析得出:在物种水平上,Shannon多样性指数(I)为0.419 2,Nei基因多样性指数(H)为0.279 6;基因分化系数Gst为0.093 5,基因流Nm为4.848 7,说明山生柳遗传分化大部分存在于相同海拔梯度内。P, H, I都反应出山生柳具有较高的遗传多样性。聚类分析显示,遗传距离与空间地理距离有显著相关性。海拔及纬度因子显著影响山生柳种群的遗传多样性水平,海拔梯度越高,山生柳的遗传多样性越丰富。

Abstract: Genetic diversity and genetic structure of Salix oritrepha (about 180 individuals in total) from nine altitudes on the Qilian mountains were analyzed by SSR (simple sequence repeat) markers. Fifteen highly stable and repeatable SSRs (selected from 47 SSRs of Populus) detected a total of 211 alleles of which 176 (85%) were polymorphic. At the species level, the Shannon index (I) was 0.419 2, Nei’s gene diversity index (H) was 0.279 6, the coefficient of gene differentiation (Gst) was 0.093 5, and the gene flow (Nm) was 4.848 7. There was more genetic differentiation within altitudes than between altitudes. All of the results include P, H, and I showed that S. oritrepha has a high genetic diversity in the Gansu Qilian mountain. Cluster analysis showed a direct correlation between genetic distance and geographic distance. Genetic diversity of S. oritrepha was notably affected by the latitude and altitude: the higher the altitude gradient, the more abundant the genetic diversity of S. oritrepha.

中图分类号:

郭敏,马彦军,李毅. 祁连山不同海拔梯度山生柳遗传多样性的SSR分析[J]. 草业学报, 2012, 21(5): 114-121.

GUO Min, MA Yan-jun, LI Yi. Analysis of genetic diversity in Salix oritrepha from different altitudes in Gansu Qilian Mountains using SSR markers[J]. Acta Prataculturae Sinica, 2012, 21(5): 114-121.

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