西藏光核桃自然居群遗传多样性的SRAP分析

草业学报 ›› 2012, Vol. 21 ›› Issue (6): 213-220.

西藏光核桃自然居群遗传多样性的SRAP分析

谭江平^1,4,曾秀丽^2,3,廖明安^1*

1.四川农业大学园艺学院,四川雅安 625014;
2.西藏自治区农牧科学院蔬菜研究所,西藏拉萨 850030;
3.四川农业大学玉米研究所,四川雅安 625014;
4.重庆市石柱土家族自治县农业委员会,重庆石柱 409100

收稿日期:2011-11-10 出版日期:2012-06-25 发布日期:2012-12-20
通讯作者: E-mail:minan0648@163.com
作者简介:谭江平(1986-),女,重庆人,在读硕士。E-mail:tanjiangping@126.com
基金资助:
西藏自治区科技厅杰出青年科学计划基金(藏科发2009(162)号)和四川农业大学博士后基金资助。

Genetic diversity of natural Prunus mira populations detected by SRAP

TAN Jiang-ping^1,4, ZENG Xiu-li^2,3, LIAO Ming-an¹

1.Horticulture College, Sichuan Agricultural University, Ya’an 625014, China;
2.Institute of Vegetables, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850030, China;
3.Maize Research Institute, Sichuan Agricultural University, Ya’an 625014, China;
4.Shizhu Agriculture Committee, Shizhu 49100, China

Received:2011-11-10 Online:2012-06-25 Published:2012-12-20

摘要/Abstract

摘要： 利用SRAP标记对来自西藏境内的4个光核桃自然居群的遗传多样性和群体遗传结构进行了分析和评价。选用20对引物对4个居群的70份材料进行扩增,检测到338个多态位点。在居群水平上,多态位点百分率为63.96%,Nei’s基因多样性和Shannon’s信息指数分别为0.194 3和0.295 5。在物种水平上,多态位点百分率为89.89%,Nei’s基因多样性和Shannon’s信息指数分别为0.246 0和0.383 9。居群间的遗传分化系数为0.170 9,居群间的基因流为1.213 0。UPGMA聚类图中,70个个体未按4个居群各自聚在一起。结果表明,光核桃遗传多样性水平较高,居群间遗传分化较小,居群间的基因交流程度较高。

Abstract: Sequence-related amplified polymorphism (SRAP) markers were used to assess the genetic diversity and population structure of four natural Prunus mira populations from Tibet. A total of 376 bands were amplified from four natural populations by 20 primers, and 338 SRAP loci were found to be polymorphic. A relatively high level of intraspecific genetic diversity was revealed: PPB (percentage of polymorphic loci)=89.89%, H (Nei’s gene diversity)=0.194 3, I (Shannon’s information index)=0.295 5 at the population level, and PPB=89.89%, H=0.246 0, I=0.383 9 at the species level. A low degree of genetic differentiation occurred among P. mira populations as indicated by a Gst (Nei’s Gst analysis) of 0.170 9 but the gene flow between populations was high (1.213 0). UPGMA (unweighted pair group method with arithmetic mean) cluster analysis showed that the 70 individuals were not clustered into four clusters corresponding to the four populations. There was relatively high genetic diversity, low genetic differentiation and a high degree of gene flow between P. mira populations.

中图分类号:

Q943

谭江平,曾秀丽,廖明安. 西藏光核桃自然居群遗传多样性的SRAP分析[J]. 草业学报, 2012, 21(6): 213-220.

TAN Jiang-ping, ZENG Xiu-li, LIAO Ming-an. Genetic diversity of natural Prunus mira populations detected by SRAP[J]. Acta Prataculturae Sinica, 2012, 21(6): 213-220.

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