草业学报 ›› 2014, Vol. 23 ›› Issue (4): 330-342.DOI: 10.11686/cyxb20140440
张振粉,南志标*
收稿日期:
2012-06-14
出版日期:
2014-08-20
发布日期:
2014-08-20
通讯作者:
E-mail:zhibiao@lzu.edu.cn
作者简介:
张振粉(1984-),男,福建霞浦人,博士。E-mail:zhangzf_10@lzu.edu.cn
基金资助:
ZHANG Zhen-fen,NAN Zhi-biao
Received:
2012-06-14
Online:
2014-08-20
Published:
2014-08-20
摘要: 综述了国内外在苜蓿细菌性病害方面的研究概况。全世界现已报道9种苜蓿细菌性病害,它们是由8种细菌病原引起的,这些细菌分属于6个属。其中由革兰氏阳性细菌引起的病害1种,即苜蓿细菌性萎蔫病(Clavibacter michiganensisi subsp. insidiosus);革兰氏阴性菌引起的病害有8种,即细菌性芽萎蔫病(Erwinia persicina),细菌性芽腐烂病(Erwinia chrysanthemi),根癌土壤杆菌(Agrobacterium tumefaciens),冠腐和根腐综合病(Pseudomonas viridiflava),细菌性叶斑病和细菌性猝倒病为同一病原(Xanthomonas campestris pv. alfalfae),细菌性茎疫病(Pseudomonas syringae pv. syringae)和矮化病(Xylella fastidiosa)。我国已报道危害较轻的细菌性叶斑病(X. campestris pv. alfalfae)和细菌性茎疫病(P. syringae pv. syringae)以及2012年新报道的细菌性芽萎蔫病(E. persicina)。本文归纳了苜蓿细菌性病害的种类及其特征;列举了病害的分布及其寄主范围;详述了它们的症状识别与病原表型特征;介绍了当前常见的苜蓿细菌病原鉴定方法,即形态、生理和生化表型特征鉴定,系统发育如16S rRNA序列分析以及自动化数值分析如Biolog自动鉴定系统。
中图分类号:
张振粉,南志标. 苜蓿细菌性病害研究进展[J]. 草业学报, 2014, 23(4): 330-342.
ZHANG Zhen-fen,NAN Zhi-biao. Research progress on alfalfa bacterial diseases[J]. Acta Prataculturae Sinica, 2014, 23(4): 330-342.
Reference:[1]Chen W, Shen Y Y, Robertson M J, et al. Simulation analysis of lucerne-wheat crop rotation on the Loess Plateau of Northern China[J]. Field Crops Research, 2008, 108: 179-187.[2]Guo Y X, Nan Z B, Wang C Z, et al. Progress in research on root invading fungi of Medicago sativa[J]. Acta Prataculturae Sinica, 2009, 18(5): 243-249.[3]Nan Z B. Establishing sustainable management system for diseases of pasture crops in China[J]. Acta Prataculturae Sinica, 2000, 9(2): 1-9.[4]Nan Z B. Our alfalfa diseases and integrated control system[J]. Animal Science and Veterinary Medicine, 2001, 18(4): 1-4.[5]Li C J, Nan Z B, Seed-borne fungi of lucerne and their pathogenicity to lucerne seed and seedling[J]. Acta Prataculturae Sinica, 2000, 9(1): 27-36.[6]Nan Z B. Effects of rust on Alfalfa nutrients[J].China Grassland and Forage, 1985, 2(3): 33-36.[7]Nan Z B, Yuan B H. Altay region Alfalfa Diseases[J]. Pratacultural Science, 1994, 11(4): 14-18.[8]Couture L, Dhont C, Chalifour F P, et al. Fusarium root and crown rot in alfalfa subjected to autumn harvests[J]. Canadian Journal Plant Science, 2002, 82: 621-624.[9]Pratt R G, Mc Laughlin M R, Pederson G A, et al . Pathogenicity of Macrophomina phaseolinato mature plant tissues of alfalfa and white clover[J]. Plant Disease, 1998, 82(9): 1033-1038.〖ZK〗[10]Li M Q. Comparative pathogenicity of isolates of fusarium spp and cultivars resistance in Alfalfa[J]. Grassland of China, 2003, 25(1): 39-43.[11]Hou T J, Huang H C, Fraser J. Pathogenicity of Verticillium albo-atrum and V. dahliae on fifteen species of forafe legumes[J]. Acta Phytopathologica Sinica, 1995, 25(2): 189-192.[12]Yuan Q H. Advances in alfalfa diseases in China[J]. Plant Protection, 2007, 33(1): 6-10.[13]Nemeth J, Laszlo E, Emody L. Clavibacter michiganensis ssp. insidiosus in lucerne seeds[J]. EPPO Bulletin, 1991, 21: 713-718.[14]Harighi B. Occurrence of alfalfa bacterial stem blight disease in Kurdistan Province, Iran[J]. Journal of Phytopathology, 2007, 155: 593-595.[15]Pierce L, Mc Cain A H. Alfalfa sprout rot caused by Erwinia chrysanthemi[J]. Plant Disease, 1987, 71: 786-788.[16]Lelliott R A, Stead D E. Methods for the Diagnosis of Bacterial Diseases of Plants[M]. Beccles and London: William Clowes Limited, 1987: 29.[17]Nyvall R F. Field Crop Disease (Third Edition)[M]. New York: The Wiley-Black Well Press, Ltd., 1999: 3-6.[18]Zhang Z F, Nan Z B. First report of Erwinia persicinus causing wilting of Medicago sativa sprouts in China[J]. Plant Disease, 2012, 96(3): 454.[19]Yu D B, Fang Z D. The preliminary list of Chinese plant pathogenic bacteria[J]. Journal of Agricultural Sciences, 1956, 7(3): 359-363.[20]Fang Z D, Ren X Z. Notes on list of bacterial plant pathogens in China[J]. Journal of Nanjing Agricultural University, 1992, 15(4): 1-6.[21]Liu R. Grassland protection science (Volume III), pasture Pathology (Second Edition)[M]. Beijing: China Agriculture Press, 1984.[22]Jones F R. A new bacterial disease of alfalfa[J]. Phytopathology, 1925, 15: 243-244.[23]Dong X Z, Cai M Y. Common Bacterial System Identification Manual[M]. Beijing: Science Press, 2001.[24]Liu R. Alfalfa bacterial blight[J]. Abroad Animal Husbandry · Grassland, 1981,(1): 1-6.[25]González A J, Tello J C, de Cara M. First report of Erwinia persicina from Phaseolus vulgaris in Spain[J]. Plant Disease, 2005, 89(1): 109.[26]González A J, Tello J C, Rodicio M R. Erwinia persicina causing chlorosis and necrotic spots in leaves and tendrils ofPisum sativumin Southeastern Spain[J]. Plant Disease, 2007, 91(4): 460.[27]Cating R A, Hong J C, Palmateer A J, et al. First report of bacterial soft rot on Vanda orchids caused by Dickeya chrysanthemi (Erwinia chrysanthemi) in the United States[J]. Plant Disease, 2008, 92(6): 977.[28]Myung I S, Lee Y K, Lee S W, et al. A new disease, bacterial leaf spot of rape, caused by atypical Pseudomonas viridiflava in South Korea[J]. Plant Disease, 2010, 94(9): 1164.[29]Martín-Sanz A, Palomo J L, Pérez de la Vega M, et al. First report of bacterial blight caused by Pseudomonas viridiflava on pea in Spain[J]. Plant Disease, 2010, 94(1): 128.[30]González A J, Rodicio M R. Pseudomonas viridiflava causing necrotic leaf spots and defoliation on Hebe spp. in Northern Spain[J]. Plant Disease, 2006, 90(9): 1143-1149.[31]Mirik M, Aysan Y, Cetinkaya-Yildiz R, et al. Watermelon as a new host of Pseudomonas viridiflava, causal agent of leaf and stem necrosis, discovered in Turkey[J]. Plant Disease, 2004, 88(8): 907.[32]Balestra G M, Mazzaglia A, Rossetti A. Outbreak of bacterial blossom blight caused by Pseudomonas viridiflava on Actinidia chinensis kiwifruit plants in Italy[J]. Plant Disease, 2008, 92(12): 1707.[33]Gitaitis R, Sumner D, Gay D, et al. Bacterial streak and bulb rot of onion: I. A diagnostic medium for the semiselective isolation and enumeration of Pseudomonas viridiflava[J]. Plant Disease, 1997, 81(8): 897-900.[34]Moretti C, Fakhrand R, Buonaurio R. Calendula officinalis: A new natural host of Pseudomonas viridiflava in Italy[J]. Plant Disease, 2012, 96(2): 285.[35]Xu L H, Xie G L, Li B, et al. First report of pear blossom blast caused by Pseudomonas syringae pv. syringae in China[J]. Plant Disease, 2008, 92(5): 832.[36]Scheck H J, Canfield M L, Pscheidt J W, et al. Rapid evaluation of pathogenicity inPseudomonas syringae pv. syringae with a lilac tissue culture bioassay and syringomycin DNA probes[J]. Plant Disease, 1997, 81(8): 905-910.[37]Langston D B, Sanders F H, Brock J H, et al. First report of a field outbreak of a bacterial leaf spot of cantaloupe and squash caused by Pseudomonas syringae pv. syringae in Georgia[J]. Plant Disease, 1997, 87(5): 600.[38]Garibaldi A, Minuto A, Scortichini M, et al. First report of syringae leaf spot caused by Pseudomonas syringae pv. syringae on tomato in Italy[J]. Plant Disease, 2007, 91(11): 1518.[39]Bobev S G, Baeyen S, Vaerenbergh J V, et al. First record of bacterial blight caused by Pseudomonas syringae pv. syringae on Pyracantha coccinea and an Amelanchier sp. in Bulgaria[J]. Plant Disease, 2008, 92(8): 1251.[40]Lenssen A W, Sorensen E L, Posler G L, et al. Forage quality of alfalfa protected by resistance to bacterial leaf spot[J]. Animal Feed Science and Technology, 1992, 39: 61-70.[41]Moffett M L, Irwin J A G. Bacterial leaf and stem spot (Xanthomonas alfalfae) of lucerne in Queensland[J]. Australian Journal of Experimental Agriculture and Animal Husbandry, 1975, 15: 223-226.[42]Wang Z X. Alfalfa bacterial leaf spot[J]. Journal of Microbiology, 2002, 22(3): 64.[43]Li C J, Nan Z B. Alfalfa and Sudan grass disease and its prevention[A]. View: Nan Z B, Li C J. Study of Chinese Grass Crops Pathology[M]. Beijing: Ocean Press, 2003.[44]OEPP/EPPO. Data sheets on quarantine Pests No. 49, Clavibacter michiganensis subsp. insidiosus[R]. Bulletin OEPP/EPPO Bulletin, 1992: 1-4.[45]Hernandez-Martinez R, Pinckard T R, Costa H S, et al. Discovery and characterization of Xylella fastidiosa strains in southern California causing mulberry leaf scorch[J]. Plant Disease, 2006, 90(9): 1143-1149.[46]Smith D L, Dominiak-Olson J, Sharber C D. First report of Pierce's disease of grape caused by Xylella fastidiosa in Oklahoma[J]. Plant Disease, 2009, 93(7): 762.[47]Huang Q, Brlansky R H, Barnes L, et al. First report of oleander leaf scorch caused by Xylella fastidiosa in Texas[J]. Plant Disease, 2004, 88(9): 1049.[48]Randall J J, French J, Yao S, et al. First report of Xylella fastidiosa in peach in New Mexico[J]. Plant Disease, 2011, 95(7): 871.[49]Rodríguez C M, Obando J J, Villalobos W, et al. First report of Xylella fastidiosa infecting coffee in Costa Rica[J]. Plant Disease, 2001, 85(9): 1027.[50]Hernandez-Martinez R, Costa H S, Dumenyo C K, et al. Differentiation of strains of Xylella fastidiosa infecting grape, almonds, and oleander using a multiprimer PCR assay[J]. Plant Disease, 2006, 90(11): 1382-1388.[51]Barnard E L, Ash E C, Hopkins D L, et al. Distribution of Xylella fastidiosa in oaks in Florida and its association with growth decline in Quercus laevis[J]. Plant Disease, 1998, 82(5): 569-572.[52]Montero-Astúa M, SaboríoRG, Chacón-Díaz C, et al. First report of Xylella fastidiosa in avocado in Costa Rica[J]. Plant Disease, 2008, 92(1): 175.[53]Aguilar E, Villalobos W, Moreira L, et al. First report of Xylella fastidiosa infecting citrus in Costa Rica[J]. Plant Disease, 2005, 89(6): 687.[54]OEPP/EPPO. Clavibacter michiganensis ssp. insidiosus[J]. OEPP/EPPO Bulletin, 2010, 40: 353-364.[55]Tao T S, Yang R F, Dong X Z. Prokaryote Phylogeny[M]. Beijing: Chemical Industry Press, 2007.[56]Busse H J, Denner E B M, Lubitz W. Classification and identification of bacteria: current approaches to an old problem. Overview of methods used in bacterial systematics[J]. Journal of Biotechnology, 1996, 47: 3-38.[57]Hugh R, Leifson E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram negative bacteria[J]. Journal of Bacteriology, 1953, 66: 24-26. [58]Kovacs N. Identification of Pseudomonas pyocyanae by the oxidase reaction[J]. Nature (London), 1956, 178: 703.[59]Gaby W L, Hadley L. Practical laboratory test for the identification of Pseudomonas aeruginosa[J]. Journal of Bacteriology, 1957, 74: 356.[60]Cowan S T, Steel K J. Comparison of differentiating criteria for staphylococci and micrococci[J]. Journal of Bacteriology, 1964, 88: 804.[61]Gagnon M, Hunting W M. New method for the catalase determination[J]. Analytical Chemistry, 1959, 31: 144.[62]Cerny G. Method for distinction of the gram negative from gram positive bacteria[J]. European Journal of Applied Microbiology, 1976, 3: 223-225.[63]Manafi M, Kneifel W. Rapid methods for differentiating gram positive from gram-negative aerobic and facultative anaerobic bacteria[J]. Journal of Applied Bacteriology, 1990, 69: 822-827.[64]Gregersen T. Rapid method for distinction of gram-negative from gram-positive bacteria[J]. European Journal of Applied Microbiology and Biotechnology, 1978, 5: 123-127.[65]Halebian S, Harris B, Finegold M, et al. Rapid method that aids in distinguishing gram-positive from gram-negative anaerobic bacteria[J]. Journal of Clinical Microbiology, 1981, 13: 444-448.[66]Bourgault A M, Lamothe F. Evaluation of the KOH test and the antibiotic disk test in routine clinical anaerobic bacteriology[J]. Journal of Clinical Microbiology, 1988, 26: 2144-2146.[67]Brenner D J, Krieg N R, Staley J T. Bergey’s Manual of Sytematic Bacteriology (Second Edition, Part B)[M]. East Lansing: Springer Press, 2005.[68]Chen X R. Relationship between degraded grassland and microorganism of steppe in Gansu East as well as phylogenetic analysis and identification of the dominant mictobes[D]. Gansu: Gansu Agricultural University, 2003.[69]Leblond B, Philippe N, Mangin I, et al. 16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reaeal inter and intraspeciffic Bifidobacterium phylogeny[J]. International Journal of Systematic Bacteriology, 1996, 46: 102.[70]Matsunaga T, Takeyama H, Nakayama H. 16S rRNA Targeted identification of cyanobacterial genera using oligonucleotide-probes immobilized on bacterial magnetic particles[J]. Journal of Applied Phycology, 2001, 13: 389-394.[71]James G. Universal bacterial identification by PCR and DNA sequencing of 16S rRNA gene[J]. PCR for Clinical Microbiology, 2010, 3: 209-214.[72]Marcheggiani S, Iaconelli M, Dangelo A, et al. Microbiological and 16S rRNA analysis of sulphite reducing clostridia from river sediments in central Italy[J]. BMC Microbiology, 2008, 8: 171.[73]Qi Y X, Zhu S F, Xie Y X, et al. Cloning and sequencing of a 16S rDNA fragment of Clavibacter michiganensis subsp. insidiosus[J]. Acta Prataculturae Sinica, 2006, 15(3): 123-127.[74]Xie G L, Zhu G N, Ren X P. Diversity of pathogenic bacteria from rice seeds[J]. Acta Phytopathologica Sinica, 2002, 32(2): 114-121.[75]Kaneshiro W S, Burger M, Vine B G, et al. Characterization of Erwinia chrysanthemi from a bacterial heart rot of pineapple outbreak in Hawaii[J]. Plant Disease (eXtra), 2008, 92: 1444-1450. [76]Zhang R W, Zhang T, Xu W Y. The ecological thoughts in chinese traditional agriculture and its utilization[J]. Acta Ecologica Sinica, 1996, 16: 100-106.[77]Neergaard P. Seed Pathology[M]. London: The MacMillan Press, Ltd., 1977: 118-146.[78]O’Hara C M, Steigerwalt A G, Hill B C, et al. First report of a human isolate of Erwinia persicinus[J]. Journal of Clinical Microbiology, 1998, 36: 248-250.参考文献:[1]Chen W, Shen Y Y, Robertson M J, et al. Simulation analysis of lucerne-wheat crop rotation on the Loess Plateau of Northern China[J]. Field Crops Research, 2008, 108: 179-187.[2]郭玉霞, 南志标, 王成章, 等.苜蓿根部入侵真菌研究进展[J].草业学报, 2009, 18(5): 243-249.[3]南志标.建立中国的牧草病害可持续管理体系[J].草业学报, 2000, 9(2): 1-9.[4]南志标.我国的苜蓿病害及其综合防治体系[J]. 动物科学与动物医学, 2001, 18(4): 1-4.[5]李春杰, 南志标.苜蓿种带真菌及其致病性测定[J].草业学报, 2000, 9(1): 27-36.[6]南志标.锈病对紫花苜蓿营养成分的影响[J].中国草原与牧草, 1985, 2(3): 33-36.[7]南志标, 员宝华.新疆阿勒泰地区苜蓿病害[J].草业科学, 1994, 11(4): 14-18.[8]Couture L, Dhont C, Chalifour F P, et al. Fusariumroot and crown rot in alfalfa subjected to autumn harvests[J]. Canadian Journal Plant Science, 2002, 82: 621-624.[9]Pratt R G, Mc Laughlin M R, Pederson G A, et al. Pathogenicity of Macrophomina phaseolinato mature plant tissues of alfalfa and white clover[J]. Plant Disease, 1998, 82(9): 1033-1038.[10]李敏权.苜蓿根和根颈腐烂病病原致病性及品种抗病性研究[J].中国草地, 2003, 25(1): 39-43.[11]侯天爵, Huang H C, Fraser J. 两种轮枝菌对15种豆科牧草的致病性[J].植物病理学报, 1995, 25(2): 189-192.[12]袁庆华.我国苜蓿病害研究进展[J].植物保护, 2007, 33(1): 6-10.[13]Nemeth J, Laszlo E, Emody L. Clavibacter michiganensisssp. insidiosusin lucerne seeds[J]. EPPO Bulletin, 1991, 21: 713-718.[14]Harighi B. Occurrence of alfalfa bacterial stem blight disease in Kurdistan Province, Iran[J]. Journal of Phytopathology, 2007, 155: 593-595.[15]Pierce L, Mc Cain A H. Alfalfa sprout rot caused by Erwinia chrysanthemi[J]. Plant Disease, 1987, 71: 786-788.[16]Lelliott R A, Stead D E. Methods for the Diagnosis of Bacterial Diseases of Plants[M]. Beccles and London: William Clowes Limited, 1987: 29.[17]Nyvall R F. Field Crop Disease (Third Edition)[M]. New York: The Wiley-Black Well Press, Ltd., 1999: 3-6.[18]Zhang Z F, Nan Z B. First report of Erwinia persicinuscausing wilting of Medicago sativasprouts in China[J]. Plant Disease, 2012, 96(3): 454.[19]俞大绂, 方中达. 中国植物病原细菌的初步名录[J]. 农业学报, 1956, 7(3): 359-363.[20]方中达, 任欣正. 中国植物病原细菌名录[J]. 南京农业大学学报, 1992, 15(4): 1-6.[21]刘若. 草原保护学(第三分册), 牧草病理学(第二版)[M]. 北京:中国农业出版社, 1984.[22]Jones F R. A new bacterial disease of alfalfa[J]. Phytopathology, 1925, 15: 243-244.[23]东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001.[24]刘若. 苜蓿细菌性凋萎病[J]. 国外畜牧学·草原, 1981, (1): 1-6.[25]González A J, Tello J C, de Cara M. First report of Erwinia persicina fromPhaseolus vulgaris in Spain[J]. Plant Disease, 2005, 89(1): 109.[26]González A J, Tello J C, Rodicio M R. Erwinia persicina causing chlorosis and necrotic spots in leaves and tendrils of Pisum sativumin Southeastern Spain[J]. Plant Disease, 2007, 91(4): 460.[27]Cating R A, Hong J C, Palmateer A J, et al. First report of bacterial soft rot on Vanda orchids caused by Dickeya chrysanthemi (Erwinia chrysanthemi) in the United States[J]. Plant Disease, 2008, 92(6): 977.[28]Myung I S, Lee Y K, Lee S W, et al. A new disease, bacterial leaf spot of rape, caused by atypicalPseudomonas viridiflavain South Korea[J]. Plant Disease, 2010, 94(9): 1164.[29]Martín-Sanz A, Palomo J L, Pérez de la Vega M, et al. First report of bacterial blight caused by Pseudomonas viridiflava on pea in Spain[J]. Plant Disease, 2010, 94(1): 128.[30]González A J, Rodicio M R. Pseudomonas viridiflavacausing necrotic leaf spots and defoliation onHebespp. in Northern Spain[J]. Plant Disease, 2006, 90(9): 1143-1149.[31]Mirik M, Aysan Y, Cetinkaya-Yildiz R, et al. Watermelon as a new host of Pseudomonas viridiflava, causal agent of leaf and stem necrosis, discovered in Turkey[J]. Plant Disease, 2004, 88(8): 907.[32]Balestra G M, Mazzaglia A, Rossetti A. Outbreak of bacterial blossom blight caused by Pseudomonas viridiflavaon Actinidia chinensiskiwifruit plants in Italy[J]. Plant Disease, 2008, 92(12): 1707.[33]Gitaitis R, Sumner D, Gay D, et al. Bacterial streak and bulb rot of onion: I. A diagnostic medium for the semiselective isolation and enumeration of Pseudomonas viridiflava[J]. Plant Disease, 1997, 81(8): 897-900.[34]Moretti C, Fakhrand R, Buonaurio R. Calendula officinalis: A new natural host of Pseudomonas viridiflavain Italy[J]. Plant Disease, 2012, 96(2): 285.[35]Xu L H, Xie G L, Li B, et al. First report of pear blossom blast caused by Pseudomonas syringae pv.syringae in China[J]. Plant Disease, 2008, 92(5): 832.[36]Scheck H J, Canfield M L, Pscheidt J W, et al. Rapid evaluation of pathogenicity in Pseudomonas syringae pv. syringae with a lilac tissue culture bioassay and syringomycin DNA probes[J]. Plant Disease, 1997, 81(8): 905-910.[37]Langston D B, Sanders F H, Brock J H, et al. First report of a field outbreak of a bacterial leaf spot of cantaloupe and squash caused by Pseudomonas syringae pv. syringae in Georgia[J]. Plant Disease, 1997, 87(5): 600.[38]Garibaldi A, Minuto A, Scortichini M, et al. First report of syringae leaf spot caused byPseudomonas syringae pv.syringae on tomato in Italy[J]. Plant Disease, 2007, 91(11): 1518.[39]Bobev S G, Baeyen S, Vaerenbergh J V, et al. First record of bacterial blight caused byPseudomonas syringae pv.syringae on Pyracantha coccinea and an Amelanchier sp. in Bulgaria[J]. Plant Disease, 2008, 92(8): 1251.[40]Lenssen A W, Sorensen E L, Posler G L, et al. Forage quality of alfalfa protected by resistance to bacterial leaf spot[J]. Animal Feed Science and Technology, 1992, 39: 61-70.[41]Moffett M L, Irwin J A G. Bacterial leaf and stem spot (Xanthomonas alfalfae) of lucerne in Queensland[J]. Australian Journal of Experimental Agriculture and Animal Husbandry, 1975, 15: 223-226.[42]王政选. 苜蓿细菌性叶斑病[J]. 微生物学杂志, 2002, 22(3): 64.[43]李春杰, 南志标. 新疆苜蓿和苏丹草病害及其防治[A]. 见: 南志标, 李春杰. 中国草类作物病理学研究[M]. 北京: 海洋出版社, 2003.[44]OEPP/EPPO. Data sheets on quarantine Pests No. 49, Clavibacter michiganensis subsp. insidiosus[R]. Bulletin OEPP/EPPO Bulletin, 1992: 1-4.[45]Hernandez-Martinez R, Pinckard T R, Costa H S, et al. Discovery and characterization of Xylella fastidiosastrains in southern California causing mulberry leaf scorch[J]. Plant Disease, 2006, 90(9): 1143-1149.[46]Smith D L, Dominiak-Olson J, Sharber C D. First report of Pierce’s disease of grape caused byXylella fastidiosa in Oklahoma[J]. Plant Disease, 2009, 93(7): 762.[47]Huang Q, Brlansky R H, Barnes L, et al. First report of oleander leaf scorch caused byXylella fastidiosa in Texas[J]. Plant Disease, 2004, 88(9): 1049.[48]Randall J J, French J, Yao S, et al. First report of Xylella fastidiosa in peach in New Mexico[J]. Plant Disease, 2011, 95(7):871.[49]Rodríguez C M, Obando J J, Villalobos W, et al. First report ofXylella fastidiosa infecting coffee in Costa Rica[J]. Plant Disease, 2001, 85(9): 1027.[50]Hernandez-Martinez R, Costa H S, Dumenyo C K, et al. Differentiation of strains of Xylella fastidiosa infecting grape, almonds, and oleander using a multiprimer PCR assay[J]. Plant Disease, 2006, 90(11): 1382-1388.[51]Barnard E L, Ash E C, Hopkins D L, et al. Distribution ofXylella fastidiosain oaks in Florida and its association with growth decline in Quercus laevis[J]. Plant Disease, 1998, 82(5): 569-572.[52]Montero-Astúa M, Saborío-R G, Chacón-Díaz C, et al. First report ofXylella fastidiosa in avocado in Costa Rica[J]. Plant Disease, 2008, 92(1): 175.[53]Aguilar E, Villalobos W, Moreira L, et al. First report ofXylella fastidiosa infecting citrus in Costa Rica[J]. Plant Disease, 2005, 89(6): 687.[54]OEPP/EPPO. Clavibacter michiganensis ssp.insidiosus[J]. OEPP/EPPO Bulletin, 2010, 40: 353-364.[55]陶天申, 杨瑞馥, 东秀珠. 原核生物系统学[M]. 北京: 化学工业出版社, 2007.[56]Busse H J, Denner E B M, Lubitz W. Classification and identification of bacteria: current approaches to an old problem. Overview of methods used in bacterial systematics[J]. Journal of Biotechnology, 1996, 47: 3-38.[57]Hugh R, Leifson E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram negative bacteria[J]. Journal of Bacteriology, 1953, 66: 24-26. [58]Kovacs N. Identification of Pseudomonas pyocyanae by the oxidase reaction[J]. Nature (London), 1956, 178: 703.[59]Gaby W L, Hadley L. Practical laboratory test for the identification ofPseudomonas aeruginosa[J]. Journal of Bacteriology, 1957, 74: 356.[60]Cowan S T, Steel K J. Comparison of differentiating criteria for staphylococci and micrococci[J]. Journal of Bacteriology, 1964, 88: 804.[61]Gagnon M, Hunting W M. New method for the catalase determination[J]. Analytical Chemistry, 1959, 31: 144.[62]Cerny G. Method for distinction of the gram-negative from gram-positive bacteria[J]. European Journal of Applied Microbiology, 1976, 3: 223-225.[63]Manafi M, Kneifel W. Rapid methods for differentiating gram-positive from gram-negative aerobic and facultative anaerobic bacteria[J]. Journal of Applied Bacteriology, 1990, 69: 822-827.[64]Gregersen T. Rapid method for distinction of gram-negative from gram-positive bacteria[J]. European Journal of Applied Microbiology and Biotechnology, 1978, 5: 123-127.[65]Halebian S, Harris B, Finegold M, et al. Rapid method that aids in distinguishing gram-positive from gram-negative anaerobic bacteria[J]. Journal of Clinical Microbiology, 1981, 13: 444-448.[66]Bourgault A M, Lamothe F. Evaluation of the KOH test and the antibiotic disk test in routine clinical anaerobic bacteriology[J]. Journal of Clinical Microbiology, 1988, 26: 2144-2146.[67]Brenner D J, Krieg N R, Staley J T. Bergey’s Manual of Sytematic Bacteriology (Second Edition, Part B)[M]. East Lansing: Springer Press, 2005.[68]陈秀蓉. 陇东典型草原草地退化与微生物相关性及其优势菌系统发育分析与鉴定[D]. 甘肃: 甘肃农业大学, 2003.[69]Leblond B, Philippe N, Mangin I, et al. 16S rRNA and 16S to 23S internal transcribed spacer sequence analyses reaeal inter and intraspeciffic Bifidobacterium phylogeny[J]. International Journal of Systematic Bacteriology, 1996, 46: 102.[70]Matsunaga T, Takeyama H, Nakayama H. 16S rRNA-Targeted identification of cyanobacterial genera using oligonucleotide-probes immobilized on bacterial magnetic particles[J]. Journal of Applied Phycology, 2001, 13: 389-394.[71]James G. Universal bacterial identification by PCR and DNA sequencing of 16S rRNA gene[J]. PCR for Clinical Microbiology, 2010, 3: 209-214.[72]Marcheggiani S, Iaconelli M, Dangelo A, et al. Microbiological and 16S rRNA analysis of sulphite-reducing clostridia from river sediments in central Italy[J]. BMC Microbiology, 2008, 8: 171.[73]漆艳香, 朱水芳, 谢艺贤, 等. 苜蓿细菌性萎蔫病菌16S rDNA片段的克隆及序列分析(简报)[J]. 草业学报, 2006, 15(3): 123-127.[74]谢关林, 朱国念, 任小平. 浙江水稻稻种病原细菌多样性研究[J]. 植物病理学报, 2002, 32(2): 114-121.[75]Kaneshiro W S, Burger M, Vine B G, et al. Characterization ofErwinia chrysanthemi from a bacterial heart rot of pineapple outbreak in Hawaii[J]. Plant Disease (e-Xtra), 2008, 92: 1444-1450. [76]张壬午, 张彤, 许文瑛. 中国传统农业中的生态观及其在技术上的应用[J]. 生态学报, 1996, 16: 100-106.[77]Neergaard P. Seed Pathology[M]. London: The MacMillan Press, Ltd., 1977: 118-146.[78]O’Hara C M, Steigerwalt A G, Hill B C, et al. First report of a human isolate of Erwinia persicinus[J]. Journal of Clinical Microbiology, 1998, 36: 248-250. |
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