苜蓿黄酮对雌性大鼠生长和繁殖性能的影响

摘要/Abstract

摘要： 为了研究苜蓿黄酮对大鼠生长、繁殖及内分泌系统的影响,本实验设计了3个苜蓿黄酮剂量组(120,400,1 200 mg/kg)和2个对照组(己烯雌酚0.5 mg/kg、空白对照组)。通过化学发光法对大鼠雌二醇(E2)、催乳素(PRL)、孕酮(P)、生长激素(GH)检测,并对大鼠繁殖相关的指标,包括子宫湿重/系数、卵巢湿重/系数、采食量、日增重、产仔数、初生窝重进行测定。结果显示,低剂量的苜蓿黄酮显著提高了性成熟雌鼠的E2水平以及子宫湿重/系数(P<0.05),显著降低了卵巢湿重/系数(P<0.05)。对未性成熟雌鼠,苜蓿黄酮对激素的影响不显著,但中、低剂量组显著降低了子宫湿重(P<0.05)。此外,苜蓿黄酮和对照组比,在采食量上不存在显著差异,但性成熟大鼠的产仔数和初生窝重有提高的趋势,中、高剂量组的产仔数和空白对照比,分别提高0.4%和2.2%。中、高剂量组的初生窝重和空白对照比,分别提高5.14%和0.10%,但差异性并不显著(P>0.05)。配种前灌胃不同剂量的苜蓿黄酮在一定程度上影响了性成熟和未性成熟雌鼠的激素分泌和生殖器官的发育, 但未能明显改善雌鼠的繁殖性能。

Abstract: The impact of different doses of alfalfa flavonoids on growth, reproduction and endocrine systems in female rats was studied using three doses of alfalfa flavonoids (120, 400, and 1 200 mg/kg) and two control groups (diethylstilbestrol 0.5 mg/kg and blank control group). Estradiol (E2), prolactin (PRL), progesterone (P), and growth hormone (GH) were determined by chemiluminescence. Related data of rat reproduction were uterine wet weight/coefficient, ovarian wet weight/coefficient, feed intake, daily body gain, litter size and newborn litter weight of rats. The low doses of alfalfa flavonoids increased the E2 level and uterine wet weight/coefficient (P<0.05) but significantly (P<0.05) reduced the ovarian wet weight/coefficient in mature female rats. GH levels were not significantly different in immature female rats but the middle and high dose groups had markedly reduced uterine wet weight/coefficients (P<0.05). However, feed intake was not significantly different although litter size and newborn litter weight had an increased trend in mature female rats. Litter size of middle and high dose groups increased 0.4% and 2.2% more than the blank control group (P>0.05). Newborn litter weight of middle and high dose groups increased 5.14% and 0.10% more than the blank control group (P>0.05). Therefore, different doses of alfalfa flavonoids influenced hormone secretions and the development of the reproductive organs before mating. Alfalfa flavonoids resulted in a significantly improved reproduction performance.

中图分类号:

王伟,马登坦,翟新莹,王成章,田苗苗,严学兵. 苜蓿黄酮对雌性大鼠生长和繁殖性能的影响[J]. 草业学报, 2013, 22(2): 100-108.

WANG Wei, MA Deng-tan, ZHAI Xin-ying, WANG Cheng-zhang, TIAN Miao-miao, YAN Xue-bing. Effect of alfalfa flavonoids on growth and reproduction performance of female rats[J]. Acta Prataculturae Sinica, 2013, 22(2): 100-108.

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