抗坏血酸引发对NaCl胁迫燕麦种子活力的影响

doi:10.11686/cyxb2017324

摘要/Abstract

摘要： 以燕麦种子为材料,通过对燕麦种子进行不同浓度(0、0.5、1.0、1.5和2.0 mmol·L^-1)的抗坏血酸(AsA)引发,然后在浓度为0、50、100和200 mmol·L^-1的NaCl胁迫下发芽,并分析其发芽率(G_p)、发芽指数(G_i)及平均发芽时间(MGT)的变化,以探讨外源AsA引发对盐胁迫种子活力的影响。结果表明:燕麦种子G_p、G_i和MGT在NaCl胁迫下显著(P<0.05)降低。随AsA浓度增加,燕麦种子G_p在浓度为100和200 mmol·L^-1的NaCl胁迫下呈先升后降的趋势,其G_i呈下降趋势,其MGT则呈增大趋势,而且不同AsA和NaCl浓度及两者的交互作用均对燕麦种子G_p、G_i和MGT影响极显著(P<0.01)。NaCl胁迫降低了燕麦种子的活力水平,而外源AsA引发可提高NaCl胁迫下燕麦种子的活力,该作用与AsA和NaCl的浓度具有密切关系。试验中1.0 mmol·L^-1的AsA引发对100和200 mmol·L^-1的NaCl胁迫效果最好。然而,外源AsA引发会延长燕麦种子在NaCl胁迫下的萌发时间。

关键词: 抗坏血酸, 燕麦, 引发, 种子活力, NaCl胁迫

Abstract: An experiment was undertaken to study the effects of ascorbic acid (AsA) priming on oat seed germination and seedling growth under salt stress conditions. Oat seeds were primed with different AsA concentrations (0, 0.5, 1.0, 1.5 and 2.0 mmol·L^-1) and then germinated in different concentrations of NaCl stress (0, 50, 100 and 200 mmol·L^-1). Changes in germination percentage (G_p), germination index (G_i) and mean germination time (MGT) were analyzed. Results showed that the G_p, G_i and MGT of oat seeds declined significantly under NaCl stress. With increasing AsA concentrations, G_p at first increased and then decreased under NaCl stress at 100 and 200 mmol·L^-1. However, the seeds’ G_i declined with increasing AsA concentrations, while their MGT increased. The effects of different concentrations of AsA and NaCl and their interaction on G_p, G_i and MGT were highly significant (P<0.01). The level of oat seed vigor was decreased by NaCl stress. However, priming with exogenous AsA promoted the vigor of salt-stressed seeds and this effect was closely related to the concentrations of AsA and NaCl. Optimal effects were reached at AsA concentrations of 1.0 mmol·L^-1 under NaCl stress at 100 and 200 mmol·L^-1. However, the germination time of oat seeds was prolonged by AsA priming under NaCl stress.

Key words: ascorbic acid, oat, priming, seed vigor, NaCl stress

董秋丽,夏方山,李晓禹,王明亚,毛培胜,朱慧森,佟莉蓉,杜利霞. 抗坏血酸引发对NaCl胁迫燕麦种子活力的影响[J]. 草业学报, 2018, 27(4): 202-208.

DONG Qiu-li, XIA Fang-shan, LI Xiao-yu, WANG Ming-ya, MAO Pei-sheng, ZHU Hui-sen,TONG Li-rong, DU Li-xia. Effect of ascorbic acid priming on the vigor of oat seeds under NaCl stress[J]. Acta Prataculturae Sinica, 2018, 27(4): 202-208.

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