草业学报 ›› 2021, Vol. 30 ›› Issue (1): 59-71.DOI: 10.11686/cyxb2020321
漫静1,2,3(), 唐波2,3, 邓波1(), 李佳欢1, 何玉娟1, 张佳良1
收稿日期:
2020-07-07
修回日期:
2020-09-07
出版日期:
2021-01-20
发布日期:
2021-01-08
通讯作者:
邓波
作者简介:
Corresponding author. E-mail: dengbo@cau.edu.cn基金资助:
Jing MAN1,2,3(), Bo TANG2,3, Bo DENG1(), Jia-huan LI1, Yu-juan HE1, Jia-liang ZHANG1
Received:
2020-07-07
Revised:
2020-09-07
Online:
2021-01-20
Published:
2021-01-08
Contact:
Bo DENG
摘要:
植物根际促生菌(PGPR)是定殖在植物根际并且能够促进植物生长发育的一类有益细菌,本研究从不同地区的羊草根际分离筛选具有固氮、溶磷、分泌ACC脱氨酶和分泌生长素(IAA)能力的PGPR菌株,以期为研发PGPR为主的微生物肥料提供菌种资源。本研究从黑龙江兰西、内蒙古锡林浩特和呼伦贝尔3个地区共分离到20株具有固氮酶活性的固氮菌株,固氮酶活性范围为8.71~11.63 nmol C2H4·mL-1·h-1。通过PKO无机磷培养基和蒙金娜有机磷培养基从3个地区筛选出的溶磷圈直径与菌落直径比值(D/d值)大于1.5的溶解无机磷的PGPR和溶解有机磷的PGPR分别有26和36株,其溶磷量范围分别为7.08~82.71 μg·mL-1和1.56~32.48 μg·mL-1。筛选出60株具有ACC脱氨酶活性的PGPR菌株,酶活性范围为0.04~64.31 μmol α-KA·mg-1 Pr·h -1。挑选上述固氮酶活性较高、溶磷能力较强及ACC脱氨酶活性较高的39株PGPR菌株进行分泌IAA能力测定,结果表明这39株PGPR均具有分泌IAA的能力,分泌量范围为3.27~48.97 μg·mL-1。通过初步试管接种试验,本研究筛选出两株菌株HPS14和XPR2,可促进羊草生长。与对照 [株高、地上和地下生物量分别为(22.2±0.58) cm,(0.0461±0.0069) g和(0.0038±0.0007) g] 相比,接种菌株HPS14后羊草的株高、地上和地下生物量分别增加了49.0%,89.2%和243.1%;接种菌株XPR2后羊草的株高、地上和地下生物量分别增加了16.8%,28.9%和240.0%。经16S rRNA基因序列比对鉴定,这两株菌分别属于Inquilinus ginsengisoli和Phyllobacterium loti。本研究结果可为后续PGPR微生物肥料的研制提供菌种资源和理论依据。
漫静, 唐波, 邓波, 李佳欢, 何玉娟, 张佳良. 羊草根际促生菌的分离筛选及促生作用研究[J]. 草业学报, 2021, 30(1): 59-71.
Jing MAN, Bo TANG, Bo DENG, Jia-huan LI, Yu-juan HE, Jia-liang ZHANG. Isolation, screening and beneficial effects of plant growth-promoting rhizobacteria (PGPR) in the rhizosphere of Leymus chinensis[J]. Acta Prataculturae Sinica, 2021, 30(1): 59-71.
图2 不同地区羊草根际PGPR数量分布LX: 兰西 Lanxi; XLHT: 锡林浩特 Xilinhot; HLBE: 呼伦贝尔 Hulun Buir
Fig.2 The distribution of PGPR in rhizosphere of L. chinensis in different areas
图3 羊草根际固氮菌固氮酶活性不同小写字母表示不同菌株之间差异达显著水平(P<0.05)。下同。Different lowercase letters indicate significant differences among different strains (P<0.05). The same below.
Fig.3 Nitrogenase activities of nitrogen fixing strains in the rhizosphere of L. chinensis (mean±SE, n=3)
菌株 编号 Strains | 筛选地区 Screening areas | 根际部位 Parts of rhizosphere | D/d值 D/d values | 溶磷量Phosphate-solubilizing ability (μg·mL-1) | 菌株 编号 Strains | 筛选地区 Screening areas | 根际部位 Parts of rhizosphere | D/d值 D/d values | 溶磷量Phosphate-solubilizing ability (μg·mL-1) |
---|---|---|---|---|---|---|---|---|---|
XPN1 | XLHT | NRS | 1.77±0.25abcde | 31.35±3.74fg | HPS4 | HLBE | RS | 1.61±0.14bcde | 30.44±2.34fg |
XPN3 | XLHT | NRS | 1.77±0.15abcde | 18.82±3.51h | HPS10 | HLBE | RS | 1.52±0.02e | 52.51±1.28c |
XPS1 | XLHT | RS | 1.62±0.07bcde | 18.99±4.23h | HPS11 | HLBE | RS | 1.59±0.12bcde | 20.66±1.77h |
XPS7 | XLHT | RS | 1.73±0.07abcde | 14.14±2.00hi | HPS14 | HLBE | RS | 2.06±0.12abcd | 36.42±2.16ef |
XPR1 | XLHT | RP | 1.52±0.05e | 78.37±5.35a | HPS15 | HLBE | RS | 1.72±0.06bcde | 82.71±6.23a |
XPR2 | XLHT | RP | 1.56±0.15cde | 40.71±4.73de | HPR1 | HLBE | RP | 1.60±0.12bcde | 80.62±1.10a |
XPH3 | XLHT | HP | 1.89±0.11abcde | 50.23±0.59cd | HPR2 | HLBE | RP | 2.08±0.34abc | 41.32±3.08de |
XPH4 | XLHT | HP | 1.60±0.10bcde | 48.98±1.83cd | HPR3 | HLBE | RP | 1.71±0.12bcde | 22.52±0.93gh |
HPN1 | HLBE | NRS | 1.57±0.03cde | 51.29±1.71c | HPR6 | HLBE | RP | 1.53±0.03de | 69.12±3.35b |
HPN14 | HLBE | NRS | 1.61±0.06bcde | 41.56±4.69de | HPH1 | HLBE | HP | 2.10±0.26ab | 15.44±2.11hi |
HPN15 | HLBE | NRS | 1.55±0.10cde | 51.19±2.56c | HPH6 | HLBE | HP | 2.11±0.31ab | 14.10±1.50hi |
HPN17 | HLBE | NRS | 1.73±0.13abcde | 14.53±1.46hi | HPH11 | HLBE | HP | 1.65±0.12bcde | 14.67±4.22hi |
HPN18 | HLBE | NRS | 1.70±0.05bcde | 18.45±1.40h | HPH12 | HLBE | HP | 2.23±0.15a | 7.08±1.49i |
表1 不同地区羊草根际不同部位溶解无机磷细菌溶磷能力
Table 1 Phosphate-solubilizing ability of inorganic phosphorus solubilizing bacteria in different areas and different regions of L. chinensis rhizosphere (mean±SE, n=3)
菌株 编号 Strains | 筛选地区 Screening areas | 根际部位 Parts of rhizosphere | D/d值 D/d values | 溶磷量Phosphate-solubilizing ability (μg·mL-1) | 菌株 编号 Strains | 筛选地区 Screening areas | 根际部位 Parts of rhizosphere | D/d值 D/d values | 溶磷量Phosphate-solubilizing ability (μg·mL-1) |
---|---|---|---|---|---|---|---|---|---|
XPN1 | XLHT | NRS | 1.77±0.25abcde | 31.35±3.74fg | HPS4 | HLBE | RS | 1.61±0.14bcde | 30.44±2.34fg |
XPN3 | XLHT | NRS | 1.77±0.15abcde | 18.82±3.51h | HPS10 | HLBE | RS | 1.52±0.02e | 52.51±1.28c |
XPS1 | XLHT | RS | 1.62±0.07bcde | 18.99±4.23h | HPS11 | HLBE | RS | 1.59±0.12bcde | 20.66±1.77h |
XPS7 | XLHT | RS | 1.73±0.07abcde | 14.14±2.00hi | HPS14 | HLBE | RS | 2.06±0.12abcd | 36.42±2.16ef |
XPR1 | XLHT | RP | 1.52±0.05e | 78.37±5.35a | HPS15 | HLBE | RS | 1.72±0.06bcde | 82.71±6.23a |
XPR2 | XLHT | RP | 1.56±0.15cde | 40.71±4.73de | HPR1 | HLBE | RP | 1.60±0.12bcde | 80.62±1.10a |
XPH3 | XLHT | HP | 1.89±0.11abcde | 50.23±0.59cd | HPR2 | HLBE | RP | 2.08±0.34abc | 41.32±3.08de |
XPH4 | XLHT | HP | 1.60±0.10bcde | 48.98±1.83cd | HPR3 | HLBE | RP | 1.71±0.12bcde | 22.52±0.93gh |
HPN1 | HLBE | NRS | 1.57±0.03cde | 51.29±1.71c | HPR6 | HLBE | RP | 1.53±0.03de | 69.12±3.35b |
HPN14 | HLBE | NRS | 1.61±0.06bcde | 41.56±4.69de | HPH1 | HLBE | HP | 2.10±0.26ab | 15.44±2.11hi |
HPN15 | HLBE | NRS | 1.55±0.10cde | 51.19±2.56c | HPH6 | HLBE | HP | 2.11±0.31ab | 14.10±1.50hi |
HPN17 | HLBE | NRS | 1.73±0.13abcde | 14.53±1.46hi | HPH11 | HLBE | HP | 1.65±0.12bcde | 14.67±4.22hi |
HPN18 | HLBE | NRS | 1.70±0.05bcde | 18.45±1.40h | HPH12 | HLBE | HP | 2.23±0.15a | 7.08±1.49i |
菌株 编号 Strains | 筛选地区 Screening areas | 根际部位 Parts of rhizosphere | D/d值 D/d values | 溶磷量Phosphate-solubilizing ability (μg·mL-1) | 菌株 编号 Strains | 筛选地区 Screening areas | 根际部位 Parts of rhizosphere | D/d值 D/d values | 溶磷量Phosphate-solubilizing ability (μg·mL-1) |
---|---|---|---|---|---|---|---|---|---|
LMR3 | LX | RP | 2.18±0.20abc | 21.26±0.16defg | HMN9 | HLBE | NRS | 2.47±0.18ab | 24.53±1.55cde |
XMN2 | XLHT | NRS | 1.63±0.39c | 21.61±2.71defg | HMN11 | HLBE | NRS | 1.82±0.47abc | 3.87±0.71j |
XMN4 | XLHT | NRS | 1.53±0.19c | 4.98±1.07j | HMN13 | HLBE | NRS | 1.53±0.24c | 15.63±2.21hi |
XMS1 | XLHT | RS | 1.96±0.04abc | 26.70±0.60bcd | HMN15 | HLBE | NRS | 1.85±0.10abc | 4.19±0.78j |
XMS3 | XLHT | RS | 2.08±0.17abc | 26.70±0.82bcd | HMN16 | HLBE | NRS | 2.60±0.12a | 19.54±4.39efgh |
XMS8 | XLHT | RS | 2.11±0.36abc | 5.49±1.10j | HMN17 | HLBE | NRS | 2.20±0.35abc | 16.48±3.06ghi |
XMS9 | XLHT | RS | 2.08±0.17abc | 1.88±0.28j | HMS1 | HLBE | RS | 2.02±0.38abc | 3.49±1.07j |
XMS11 | XLHT | RS | 2.00±0.10abc | 22.06±0.22defg | HMS6 | HLBE | RS | 1.93±0.19abc | 2.98±0.96j |
XMS12 | XLHT | RS | 1.68±0.08bc | 23.78±0.35cdef | HMS7 | HLBE | RS | 2.12±0.06abc | 25.13±0.34cde |
XMS15 | XLHT | RS | 2.22±0.20abc | 31.19±2.05ab | HMS19 | HLBE | RS | 1.66±0.30bc | 12.34±2.31i |
XMS16 | XLHT | RS | 1.67±0.10bc | 22.48±2.97def | HMR1 | HLBE | RP | 1.61±0.20c | 29.29±0.18abc |
XMH1 | XLHT | HP | 2.03±0.14abc | 1.99±0.16j | HMR2 | HLBE | RP | 1.75±0.14bc | 6.58±2.70j |
HMN2 | HLBE | NRS | 1.72±0.15bc | 18.44±0.56fgh | HMH3 | HLBE | HP | 1.86±0.10abc | 21.69±0.35defg |
HMN3 | HLBE | NRS | 1.61±0.20c | 32.48±4.34a | HMH11 | HLBE | HP | 2.05±0.29abc | 1.99±0.14j |
HMN5 | HLBE | NRS | 1.60±0.21c | 5.91±0.22j | HMH14 | HLBE | HP | 1.67±0.04bc | 1.85±0.14j |
HMN6 | HLBE | NRS | 1.88±0.44abc | 4.26±1.55j | HMH17 | HLBE | HP | 1.93±0.10abc | 1.56±0.18j |
HMN7 | HLBE | NRS | 1.70±0.25bc | 1.92±0.31j | HMH21 | HLBE | HP | 1.55±0.23c | 22.23±3.35def |
HMN8 | HLBE | NRS | 1.93±0.15abc | 3.28±0.97j | HMH22 | HLBE | HP | 1.98±0.26abc | 3.13±0.74j |
表2 不同地区羊草根际不同部位溶解有机磷细菌溶磷能力
Table 2 Phosphorus solubilizing ability of organic phosphorus solubilizing bacteria in different areas and different regions of L. chinensis rhizosphere (mean±SE, n=3)
菌株 编号 Strains | 筛选地区 Screening areas | 根际部位 Parts of rhizosphere | D/d值 D/d values | 溶磷量Phosphate-solubilizing ability (μg·mL-1) | 菌株 编号 Strains | 筛选地区 Screening areas | 根际部位 Parts of rhizosphere | D/d值 D/d values | 溶磷量Phosphate-solubilizing ability (μg·mL-1) |
---|---|---|---|---|---|---|---|---|---|
LMR3 | LX | RP | 2.18±0.20abc | 21.26±0.16defg | HMN9 | HLBE | NRS | 2.47±0.18ab | 24.53±1.55cde |
XMN2 | XLHT | NRS | 1.63±0.39c | 21.61±2.71defg | HMN11 | HLBE | NRS | 1.82±0.47abc | 3.87±0.71j |
XMN4 | XLHT | NRS | 1.53±0.19c | 4.98±1.07j | HMN13 | HLBE | NRS | 1.53±0.24c | 15.63±2.21hi |
XMS1 | XLHT | RS | 1.96±0.04abc | 26.70±0.60bcd | HMN15 | HLBE | NRS | 1.85±0.10abc | 4.19±0.78j |
XMS3 | XLHT | RS | 2.08±0.17abc | 26.70±0.82bcd | HMN16 | HLBE | NRS | 2.60±0.12a | 19.54±4.39efgh |
XMS8 | XLHT | RS | 2.11±0.36abc | 5.49±1.10j | HMN17 | HLBE | NRS | 2.20±0.35abc | 16.48±3.06ghi |
XMS9 | XLHT | RS | 2.08±0.17abc | 1.88±0.28j | HMS1 | HLBE | RS | 2.02±0.38abc | 3.49±1.07j |
XMS11 | XLHT | RS | 2.00±0.10abc | 22.06±0.22defg | HMS6 | HLBE | RS | 1.93±0.19abc | 2.98±0.96j |
XMS12 | XLHT | RS | 1.68±0.08bc | 23.78±0.35cdef | HMS7 | HLBE | RS | 2.12±0.06abc | 25.13±0.34cde |
XMS15 | XLHT | RS | 2.22±0.20abc | 31.19±2.05ab | HMS19 | HLBE | RS | 1.66±0.30bc | 12.34±2.31i |
XMS16 | XLHT | RS | 1.67±0.10bc | 22.48±2.97def | HMR1 | HLBE | RP | 1.61±0.20c | 29.29±0.18abc |
XMH1 | XLHT | HP | 2.03±0.14abc | 1.99±0.16j | HMR2 | HLBE | RP | 1.75±0.14bc | 6.58±2.70j |
HMN2 | HLBE | NRS | 1.72±0.15bc | 18.44±0.56fgh | HMH3 | HLBE | HP | 1.86±0.10abc | 21.69±0.35defg |
HMN3 | HLBE | NRS | 1.61±0.20c | 32.48±4.34a | HMH11 | HLBE | HP | 2.05±0.29abc | 1.99±0.14j |
HMN5 | HLBE | NRS | 1.60±0.21c | 5.91±0.22j | HMH14 | HLBE | HP | 1.67±0.04bc | 1.85±0.14j |
HMN6 | HLBE | NRS | 1.88±0.44abc | 4.26±1.55j | HMH17 | HLBE | HP | 1.93±0.10abc | 1.56±0.18j |
HMN7 | HLBE | NRS | 1.70±0.25bc | 1.92±0.31j | HMH21 | HLBE | HP | 1.55±0.23c | 22.23±3.35def |
HMN8 | HLBE | NRS | 1.93±0.15abc | 3.28±0.97j | HMH22 | HLBE | HP | 1.98±0.26abc | 3.13±0.74j |
菌株 编号 Strains | 筛选地区 Screening areas | 根际部位 Parts of rhizosphere | ACC脱氨酶活性 ACC deaminase activity | 菌株 编号 Strains | 筛选地区 Screening areas | 根际部位 Parts of rhizosphere | ACC脱氨酶活性 ACC deaminase activity | 菌株 编号 Strains | 筛选地区 Screening areas | 根际部位 Parts of rhizosphere | ACC脱氨酶活性 ACC deaminase activity |
---|---|---|---|---|---|---|---|---|---|---|---|
LAN8 | LX | NRS | 14.10 | LAH34 | LX | HP | 13.50 | HAS2 | HLBE | RS | 16.17 |
LAR1 | LX | RP | 11.87 | XAN9 | XLHT | NRS | 21.76 | HAS10 | HLBE | RS | 0.94 |
LAH7 | LX | HP | 1.97 | XAN11 | XLHT | NRS | 3.57 | HAS13 | HLBE | RS | 26.28 |
LAH9 | LX | HP | 24.42 | XAS2 | XLHT | RS | 4.12 | HAS14 | HLBE | RS | 7.02 |
LAH14 | LX | HP | 14.08 | XAS3 | XLHT | RS | 11.05 | HAS15 | HLBE | RS | 0.42 |
LAH15 | LX | HP | 28.40 | XAS4 | XLHT | RS | 5.13 | HAR4 | HLBE | RP | 27.01 |
LAH17 | LX | HP | 8.55 | XAS5 | XLHT | RS | 3.25 | HAR5 | HLBE | RP | 13.05 |
LAH18 | LX | HP | 12.38 | XAS6 | XLHT | RS | 12.03 | HAR7 | HLBE | RP | 64.31 |
LAH19 | LX | HP | 8.39 | XAS9 | XLHT | RS | 33.12 | HAR9 | HLBE | RP | 21.46 |
LAH20 | LX | HP | 7.19 | XAS11 | XLHT | RS | 1.35 | HAR11 | HLBE | RP | 6.44 |
LAH21 | LX | HP | 3.74 | XAS12 | XLHT | RS | 22.11 | HAR12 | HLBE | RP | 12.70 |
LAH22 | LX | HP | 0.04 | XAS13 | XLHT | RS | 9.10 | HAR13 | HLBE | RP | 10.62 |
LAH23 | LX | HP | 12.54 | XAS15 | XLHT | RS | 4.06 | HAR15 | HLBE | RP | 11.64 |
LAH25 | LX | HP | 28.92 | XAS18 | XLHT | RS | 4.92 | HAR20 | HLBE | RP | 21.09 |
LAH27 | LX | HP | 1.98 | XAS19 | XLHT | RS | 14.61 | HAR21 | HLBE | RP | 12.80 |
LAH28 | LX | HP | 8.18 | XAS20 | XLHT | RS | 11.29 | HAR22 | HLBE | RP | 15.43 |
LAH29 | LX | HP | 36.43 | XAR1 | XLHT | RP | 7.93 | HAR23 | HLBE | RP | 20.83 |
LAH31 | LX | HP | 14.14 | XAR2 | XLHT | RP | 0.30 | HAR24 | HLBE | RP | 8.44 |
LAH32 | LX | HP | 2.92 | XAH11 | XLHT | HP | 9.68 | HAR28 | HLBE | RP | 12.09 |
LAH33 | LX | HP | 13.95 | XAH12 | XLHT | HP | 0.47 | HAR29 | HLBE | RP | 8.43 |
表3 PGPR菌株ACC脱氨酶活性
Table 3 ACC deaminase activity of PGPR isolates (μmol α-KA·mg-1 Pr·h-1)
菌株 编号 Strains | 筛选地区 Screening areas | 根际部位 Parts of rhizosphere | ACC脱氨酶活性 ACC deaminase activity | 菌株 编号 Strains | 筛选地区 Screening areas | 根际部位 Parts of rhizosphere | ACC脱氨酶活性 ACC deaminase activity | 菌株 编号 Strains | 筛选地区 Screening areas | 根际部位 Parts of rhizosphere | ACC脱氨酶活性 ACC deaminase activity |
---|---|---|---|---|---|---|---|---|---|---|---|
LAN8 | LX | NRS | 14.10 | LAH34 | LX | HP | 13.50 | HAS2 | HLBE | RS | 16.17 |
LAR1 | LX | RP | 11.87 | XAN9 | XLHT | NRS | 21.76 | HAS10 | HLBE | RS | 0.94 |
LAH7 | LX | HP | 1.97 | XAN11 | XLHT | NRS | 3.57 | HAS13 | HLBE | RS | 26.28 |
LAH9 | LX | HP | 24.42 | XAS2 | XLHT | RS | 4.12 | HAS14 | HLBE | RS | 7.02 |
LAH14 | LX | HP | 14.08 | XAS3 | XLHT | RS | 11.05 | HAS15 | HLBE | RS | 0.42 |
LAH15 | LX | HP | 28.40 | XAS4 | XLHT | RS | 5.13 | HAR4 | HLBE | RP | 27.01 |
LAH17 | LX | HP | 8.55 | XAS5 | XLHT | RS | 3.25 | HAR5 | HLBE | RP | 13.05 |
LAH18 | LX | HP | 12.38 | XAS6 | XLHT | RS | 12.03 | HAR7 | HLBE | RP | 64.31 |
LAH19 | LX | HP | 8.39 | XAS9 | XLHT | RS | 33.12 | HAR9 | HLBE | RP | 21.46 |
LAH20 | LX | HP | 7.19 | XAS11 | XLHT | RS | 1.35 | HAR11 | HLBE | RP | 6.44 |
LAH21 | LX | HP | 3.74 | XAS12 | XLHT | RS | 22.11 | HAR12 | HLBE | RP | 12.70 |
LAH22 | LX | HP | 0.04 | XAS13 | XLHT | RS | 9.10 | HAR13 | HLBE | RP | 10.62 |
LAH23 | LX | HP | 12.54 | XAS15 | XLHT | RS | 4.06 | HAR15 | HLBE | RP | 11.64 |
LAH25 | LX | HP | 28.92 | XAS18 | XLHT | RS | 4.92 | HAR20 | HLBE | RP | 21.09 |
LAH27 | LX | HP | 1.98 | XAS19 | XLHT | RS | 14.61 | HAR21 | HLBE | RP | 12.80 |
LAH28 | LX | HP | 8.18 | XAS20 | XLHT | RS | 11.29 | HAR22 | HLBE | RP | 15.43 |
LAH29 | LX | HP | 36.43 | XAR1 | XLHT | RP | 7.93 | HAR23 | HLBE | RP | 20.83 |
LAH31 | LX | HP | 14.14 | XAR2 | XLHT | RP | 0.30 | HAR24 | HLBE | RP | 8.44 |
LAH32 | LX | HP | 2.92 | XAH11 | XLHT | HP | 9.68 | HAR28 | HLBE | RP | 12.09 |
LAH33 | LX | HP | 13.95 | XAH12 | XLHT | HP | 0.47 | HAR29 | HLBE | RP | 8.43 |
菌株编号 Strains | IAA分泌量 IAA production | 菌株编号 Strains | IAA分泌量 IAA production | 菌株编号 Strains | IAA分泌量 IAA production |
---|---|---|---|---|---|
LNR24 | 12.94±0.86fg | XMS15 | 8.18±0.19hijk | HPR3 | 5.55±0.21ijkl |
LMR3 | 48.97±3.56a | XMS16 | 7.74±1.03ijkl | HPR6 | 4.86±0.38jkl |
XPN3 | 4.11±0.07kl | XMH1 | 39.02±2.91b | HPH6 | 4.99±0.26jkl |
XPS1 | 14.68±2.06ef | HPN14 | 4.18±0.38kl | HPH11 | 5.61±0.41ijkl |
XPR1 | 7.45±1.33ijkl | HPN15 | 4.71±0.44jkl | HMN2 | 3.27±0.18l |
XPR2 | 42.05±3.13b | HPN17 | 19.88±0.12d | HMN3 | 6.12±0.34ijkl |
XPH3 | 10.04±2.16ghi | HPN18 | 4.36±0.41kl | HMN9 | 5.60±0.51ijkl |
XPH4 | 17.25±1.25de | HPS10 | 19.02±2.13d | HMN13 | 5.55±0.21ijkl |
XMN2 | 7.55±0.63ijkl | HPS11 | 12.27±1.91fgh | HMN16 | 5.42±0.51ijkl |
XMS1 | 8.76±1.02ghijk | HPS14 | 5.38±0.10ijkl | HMN17 | 5.57±0.27ijkl |
XMS3 | 5.43±0.30ijkl | HPS15 | 4.48±0.64jkl | HMS7 | 6.69±0.38ijkl |
XMS11 | 31.79±2.46c | HPR1 | 7.30±1.75ijkl | HMS19 | 17.12±2.00de |
XMS12 | 9.18±1.48ghij | HPR2 | 6.01±0.08ijkl | HMR2 | 6.08±0.06ijkl |
表4 优良PGPR菌株分泌IAA能力
Table 4 IAA production by PGPR isolates (mean±SE, n=3, μg·mL-1)
菌株编号 Strains | IAA分泌量 IAA production | 菌株编号 Strains | IAA分泌量 IAA production | 菌株编号 Strains | IAA分泌量 IAA production |
---|---|---|---|---|---|
LNR24 | 12.94±0.86fg | XMS15 | 8.18±0.19hijk | HPR3 | 5.55±0.21ijkl |
LMR3 | 48.97±3.56a | XMS16 | 7.74±1.03ijkl | HPR6 | 4.86±0.38jkl |
XPN3 | 4.11±0.07kl | XMH1 | 39.02±2.91b | HPH6 | 4.99±0.26jkl |
XPS1 | 14.68±2.06ef | HPN14 | 4.18±0.38kl | HPH11 | 5.61±0.41ijkl |
XPR1 | 7.45±1.33ijkl | HPN15 | 4.71±0.44jkl | HMN2 | 3.27±0.18l |
XPR2 | 42.05±3.13b | HPN17 | 19.88±0.12d | HMN3 | 6.12±0.34ijkl |
XPH3 | 10.04±2.16ghi | HPN18 | 4.36±0.41kl | HMN9 | 5.60±0.51ijkl |
XPH4 | 17.25±1.25de | HPS10 | 19.02±2.13d | HMN13 | 5.55±0.21ijkl |
XMN2 | 7.55±0.63ijkl | HPS11 | 12.27±1.91fgh | HMN16 | 5.42±0.51ijkl |
XMS1 | 8.76±1.02ghijk | HPS14 | 5.38±0.10ijkl | HMN17 | 5.57±0.27ijkl |
XMS3 | 5.43±0.30ijkl | HPS15 | 4.48±0.64jkl | HMS7 | 6.69±0.38ijkl |
XMS11 | 31.79±2.46c | HPR1 | 7.30±1.75ijkl | HMS19 | 17.12±2.00de |
XMS12 | 9.18±1.48ghij | HPR2 | 6.01±0.08ijkl | HMR2 | 6.08±0.06ijkl |
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