Effect of photoperiod on phytochromes and endogenous hormones of alfalfa with different fall-dormancies

doi:10.11686/cyxb20140121

Abstract

Abstract: To explore the effects of photoperiod on phytochromes and endogenous hormones of different fall-dormancy alfalfas (Medicago sativa),and to reveal the regulatory mechanisms,the expressions of PHYA (phytochrome A) and PHYB (phytochrome B) were observed by the FQ-PCR SYBR-Green I method after 35 days in three standard varieties: the fall-dormant variety Norseman(FD₁),the semi-dormant variety Dupuils (FD₅) and the non-dormant variety CUF101 (FD₉),under different photoperiods (7,10,13 and 16 h/d). Their endogenous hormone contents (GA₃,ZR,IAA and ABA) were measured by Enzyme-linked immunosorbent assay (ELISA). The mRNA expressions of PHYA and PHYB in the three alfalfa varieties increased gradually with a shortening photoperiod,especially in the fall-dormant variety Norseman. The ratios of GA₃/ABA,ZR/ABA and IAA/ABA decreased with increased photoperiod and those of the Norseman variety were the lowest: it had the slowest growth,the highest ABA level and the lowest GA₃ level. It is possible that PHYA and PHYB directly or indirectly influenced the synthesis of GA₃,ZR,IAA,ABA and further regulated fall dormancy.

CLC Number:

S816

FAN Wen-na,SUN Xiao-ge,NI Jun-xia,DU Hong-qi,SHI Ying-hua,YAN Xue-bing,WANG Cheng-zhang. Effect of photoperiod on phytochromes and endogenous hormones of alfalfa with different fall-dormancies[J]. Acta Prataculturae Sinica, 2014, 23(1): 177-184.

References

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