ISSR analysis of genetic diversity of germplasm resources of Bothriochloa ischaemum in Shanxi

doi:10.11686/cyxb20140126

Abstract

Abstract: The genetic diversities of 33 germplasm resources of Bothriochloa ischaemum in Shanxi Province were analyzed using inter-simple sequence repeat (ISSR) techniques. The genetic diversity among germplasm resources was relatively stable. Ninety-nine bands,of which 52 (52.23%) were polymorphic,were amplified using 10 pairs of ISSR primers. Analyzes by PopGen32 software showed the number of alleles (Na) was 1.5253±0.5019,the effective number of alleles (Ne) was 1.2941±0.3665,Nei’s gene diversity (H) was 0.1723±0.1983,and Shannon’s information index (I) was 0.2590±0.2841; According to the results of NTSYSpc 1.20c,the genetic similarity coefficient (GS) among 33 germplasm materials ranged from 0.8188 to 0.9801,the genetic distance (GD) ranged from 0.0200 to 0.1988. The population genetic diversity of 33 germplasm materials was low: the possible reason could be that the reproductive strategy of B. ischaemum is apomixic under natural conditions.

CLC Number:

S816

LI Yu-ying,DONG Kuan-hu. ISSR analysis of genetic diversity of germplasm resources of Bothriochloa ischaemum in Shanxi[J]. Acta Prataculturae Sinica, 2014, 23(1): 217-222.

References

Reference:
[1]Dong K H. The research of aboveground biomass ,the niche of population and pasture cultivation with Old world bluestem rangeland in Shanxi[D]. Benjing: China Agricultural University, 2004.
[2]Celarier R P. The Cyto geography of the Bothriochloa ischaemumcomplex. II. chromosome behavior[J]. American Journal of Botany, 1957, 44(9): 729-738.
[3]Brown W V, Emery W H P. Apomixis in the Gramineae, Tribe Andropogoneae: Themeda triandraand Bothriochloa ischaemum[J]. Botanical Gazette, 1957, 118(4): 246-253.
[4]Xiao F Z, Wang J L. The preliminary report about research of Ischaemum’s no apomixis phenomenon[J]. Chinese Bulletin of Botany, 1994, (S1): 73.
[5]Ma G H, Huang X L. Cytological and embryological studies on apospory in Bothriochloa ischaemumL.[J]. Acta Biologica Hungarica, 2007, 58(4): 421-429.
[6]Wet J M J D, Borgaonkar D S. Aneuploidy and apomixis in Bothriochloa and Dichanthium[J]. Botanical Gazette, 1963, 124(6):437-440.
[7]Wang H Y, Wang Z W, Li L H, et al. Reproductive tendency of clonal plants in various habitats[J]. Chinese Journal of Ecology, 2oo5, 24(6): 670-676.
[8]Gabbard B L, Fowler N L. Wide ecological amplitude of a diversity-reducing invasive grass[J]. Biological Invasions, 2007, 9(2):149-160.
[9]Dong K H, Mi J. Quantitative characters of vegetative multiplication of bothriochloa ischaemum populations[J]. Acta Agrestia Sinica, 2006, 14(3): 210-213.
[10]Zhang C S, Liu G B, Xue S, et al. Photosynthetic characteristics of Bothriochloa ischaemum under drought stress and elevated CO2 concentration[J]．Chinese Journal of Applied Ecology, 2012, 21(11): 3009-3015.
[11]Yuan Z C. Distribution pattern of species abundance in Bothriochloa ischaemun community and spatial point pattern analysis on the dominant species in the Loess Gully Region. China[D]. Yangling: Northwest A&F University, 2012.
[12]Xu W Z, Xu B C, Duan D P, et al. Study on the photosynthetic characteristics of bothriochloa ischaemum under different water and nutrient conditions Ⅲ.chlorophyll fluorescence kinetic parameters[J]. Acta Agrestia Sinica, 2011, 19(1): 31-37.
[13]Li Y Y, Dong K H, Wang R M, et al. Establishment and optimization of ISSR-PCR reaction system for bothriochloa ischaemum[J]. Chinese Journal of Grassland, 2012, 34($): 15-20.
[14]Matakis S, Overath R D, Kutil B, et al. Isolation and characterization of microsatellite markers for Bothriochloa ischaemum(Poaceae)[J]. American Journal of Botany, 2011, 98(7): 192-194.
[15]Zietkiewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat (SSR) anchored polymerase chain reaction amplification[J]. Genomics, 1994, 20(2): 176-183.
[16]Zhou Y Q, Yang Q X, Zhang G N. Application and Markers of Genetic[M]. Beijing: Chemical Industry Press, 2008: 3-12.
[17]Tian Q S, Han B, Yang J, et al. Genetic diversity analysis of 96 brome materials based on ISSR markers[J]. Chinese Journal of Grassland, 2010, 32(1): 18-25.
[18]Liu H, Mu P, Zhao G Q. A study on genetic diversity of Avena germplasm resources detected by ISSR[J]. Acta Prataculturae Sinica, 2012, 21(4): 116-124.
[19]Li H Y, Li Z Y, Shi W G, et al. The genetic diversity of three ecological Medicago ruthenica germplasms revealed by ISSR and SSR[J]. Prataculturae Sinica, 2012, 21(5): 107-113.
[20]Wei Z W. Estimates of genetic diversity on alfalfa by molecular markers base on cultivars characters[D]. Lanzhou: Gansu agricultural university, 2003.
[21]Liu C H, Zhou Z X, Zhou Y, et al. RAPD analysis of Eulaliopsis binata in different populations[J]. Acta Botanica Boreali-occidentalia Sinica, 2006, 26(5): 915-920.
[22]Guo M, Ma Y J, Li Y. Analysis of genetic diversity in Salix oritrepha from different altitudes in Gansu Qilian Mountains using SSR markers[J]. Acta Prataculturae Sinica, 2012, 21(5): 114-121.
[23]Zeng L, Yuan Q Y, Wang F, et al. Genetic diversity analysis of Agropyron germplasm resources by ISSR[J]. Acta Prataculturae Sinica, 2013, 22(1): 260-267.
[24]Sun Q, Tong H W, Wu B, et al. Genetic diversity of glycyrrhiza uralensis fisch.detected by morphological and ISSR molecular markers[J]. Journal of Plant Genetic Resources, 2007, 8(1): 56-63.
[25]Yu L, Jing Z B, Cheng J M. Genetics diversity of Stipa bungeana Trin. populations in Shanxi analyzed by ISSR markes[J]. Acta Agrestia Sinica, 2012, 20(3): 512-517.
[26]Hamrick J L, Loveless M D. Associations between the breeding system and the genetic structure of tropical tree populations[J]. American Journal of Botany, 1987, 74(5): 642.
[27]Guo Z J. Study on developmental and reproductive characteristics of main rhizomatous grass in grassland vegetation on Mongolia plateau[D]. Huhehaote: Inner Mongolia Agricultural University.
[28]Xu Q F, Dong K H, Shi S R, et al. The flora characteristics of graszing botriochloa ischaemum shrub grassland[J]. Acta Agrestia Sinica, 2004, 12(2): 136-139.
[29]Yang Y J, Xie S Q, Meng Z G, et al. Pollination ecology of coptis teeta wall.an endangered medicinal plant[J]. Acta Botanica Boreali-occidentalia Sinica, 2012, 32(7): 1372-1376.
[30]Yao J L, Hong L, Zhang Y D, et al. genetic diversity and classification of ecotypes of eulaliopsis binata via morphological traits and AFLP markers[J]. Scientia Agricultura Sinica, 2004, 37(11): 1699-1704.

参考文献：
[1]董宽虎. 山西白羊草草地生产性能、种群生态位及草地培育的研究[D]. 北京: 中国农业大学, 2004.
[2]Celarier R P. The Cyto-geography of the Bothriochloa ischaemum complex. II. chromosome behavior[J]. American Journal of Botany, 1957, 44(9): 729-738.
[3]Brown W V, Emery W H P. Apomixis in the Gramineae, Tribe Andropogoneae: Themeda triandraand Bothriochloa ischaemum[J]. Botanical Gazette, 1957, 118(4): 246-253.
[4]肖辅珍，王景林. 白羊草无融合生殖现象的研究初报[J]. 植物学通报, 1994, (S1): 73.
[5]Ma G H, Huang X L. Cytological and embryological studies on apospory in Bothriochloa ischaemum L.[J]. Acta Biologica Hungarica, 2007, 58(4): 421-429.
[6]Wet J M J D, Borgaonkar D S. Aneuploidy and apomixis in Bothriochloaand Dichanthium[J]. Botanical Gazette, 1963, 124(6): 437-440.
[7]王洪义, 王正文, 李凌浩, 等. 不同生境中克隆植物的繁殖倾向[J]. 生态学杂志, 2005, 24(6): 670-676.
[8]Gabbard B L, Fowler N L. Wide ecological amplitude of a diversity-reducing invasive grass[J]. Biological Invasions, 2007, 9(2):149-160.
[9]董宽虎, 米佳. 白羊草种群繁殖的数量特征[J]. 草地学报, 2006, 14(3): 210-213.
[10]张昌胜, 刘国彬, 薛萐, 等. 干旱胁迫和CO2浓度升高条件下白羊草的光合特征[J]. 应用生态学报, 2012, 21(11): 3009-3015.
[11]袁子成. 黄土丘陵沟壑区白羊草群落种多度格局及优势种空间点格局分析[D]. 杨凌: 西北农林科技大学, 2012.
[12]徐伟洲, 徐炳成, 段东平, 等. 不同水肥条件下白羊草光合生理生态特征研究Ⅲ.叶绿素荧光参数[J]. 草地学报, 2011, 19(1): 31-37.
[13]李钰莹, 董宽虎, 王若梦, 等. 白羊草ISSR-PCR反应体系的建立与优化[J]. 中国草地学报, 2012, 34(4): 15-20.
[14]Matakis S, Overath R D, Kutil B,et al. Isolation and characterization of microsatellite markers for Bothriochloa ischaemum (Poaceae)[J]. American Journal of Botany, 2011, 98(7): 192-194.
[15]Zietkiewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification[J]. Genomics, 1994, 20(2): 176-183.
[16]周延清, 杨清香, 张改娜. 生物遗传标记与应用[M]. 北京: 化学工业出版社, 2008: 3-12.
[17]田青松, 韩冰, 杨劼, 等. 96份雀麦属材料遗传多样性的ISSR分析[J]. 中国草地学报, 2010, 32(1): 18-25.
[18]刘欢, 慕平, 赵桂琴. 燕麦种质资源遗传多样性ISSR研究[J]. 草业学报, 2012, 21(4): 116-124.
[19]李鸿雁, 李志勇, 师文贵, 等. 3种生态型野生扁蓿豆种质资源ISSR与SSR遗传多样性分析[J]. 草业学报, 2012, 21(5): 107-113.
[20]魏臻武. 苜蓿遗传多样性分子标记及其种质资源评价[D]. 兰州: 甘肃农业大学, 2003.
[21]刘传虎, 周兆喜, 周云, 等. 不同居群龙须草RAPD分析及其分类研究[J]. 西北植物学报, 2006, 26(5): 915-920.
[22]郭敏, 马彦军, 李毅. 祁连山不同海拔梯度山生柳遗传多样性的SSR分析[J]. 草业学报, 2012, 21(5): 114-121.
[23]曾亮, 袁庆华, 王方, 等. 冰草属植物种质资源遗传多样性的ISSR分析[J]. 草业学报, 2013, 22(1): 260-267.
[24]孙群, 佟汉文, 吴波, 等. 不同种源乌拉尔甘草形态和ISSR遗传多样性研究[J]. 植物遗传资源学报, 2007, 8(1): 56-63.
[25]俞靓, 井赵斌, 程积民. 陕西省本氏针茅自然种群遗传多样性的ISSR分析[J]. 草地学报, 2012, 20(3): 512-517.
[26]Hamrick J L, Loveless M D. Associations between the breeding system and the genetic structure of tropical tree populations[J]. American Journal of Botany, 1987, 74(5): 642.
[27]国振杰. 蒙古高原草原区主要根茎禾草发育及繁殖特性的研究[D]. 呼和浩特: 内蒙古农业大学, 2007.
[28]许庆方, 董宽虎, 史书瑞, 等. 放牧利用白羊草灌丛草地的植被特征[J]. 草地学报, 2004, 12(2): 136-139.
[29]杨艳娟, 谢世清, 孟珍贵, 等. 濒危药用植物云南黄连传粉生态学研究[J]. 西北植物学报, 2012, 32(7): 1372-1376.
[30]姚家玲, 洪柳, 张友德, 等. 龙须草生态型的划分及其遗传差异的AFLP分析[J]. 中国农业科学, 2004, 37(11): 1699-1704.