Antioxidant properties of plants on different sites in the hilly-gullied Loess Plateau

doi:10.11686/cyxb20140501

Abstract

Abstract:

This study investigated antioxidant properties of main plant species on 5 different site classes (sunny or shady gully and inter-gully land or hilltops)) in the hilly-gullied Loess Plateau. From field sampling, the activities of the antioxidant enzymes-superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), the contents of two non-enzymatic antioxidants-reduced glutathione (GSH) and carotenoid (Car), and the malondialdehyde (MDA) levels of leaf tissues of nineteen species were determined. There were highly significant differences in MDA accumulation among species on each site (P<0.01). This indicated that the test species suffered varying degrees of membrane lipid peroxidation, with Buddleja alternifolia and Artemisia scoparia being highest and lowest respectively. There were significant differences in antioxidant enzyme activity or non-enzymatic antioxidant levels among sites for each test species, except for Bothriochloa ischcemum, Lespedeza davurica, Heteropappus altaicus and Sophora davidii, which suggests that these four species possessed upregulation of antioxidant enzymes and non-enzymatic antioxidant synthesis regardless of different site stresses, while Cleistogenes chinensis, Cleistogenes squarrosa, Stipa bungeana, Astragalus melilotoides, Astragalus scaberrimus, Glycyrrhiza uralensis, Potentilla tanacetifolia, A. scoparia, Artemisia vestita, Artemisia giraldii, Hippophae rhamnoides, Periploca sepium and B. alternifolia regulated their antioxidant enzymes or non-enzymatic antioxidant levels. From the botanical family perspective, antioxidant properties of the test species were distinct. CAT and SOD were respectively produced by five composite and five leguminous plant species to reduce damage from reactive oxygen species. Four graminaceous plants were able to attain high SOD, POD activities and Car contents to maintain a low level of membrane lipid peroxidation. Because of different carbon assimilation pathways, antioxidant properties of the test species were also distinct. In particular, C₄ plants had higher GSH and Car contents than C₃ plants. As assessed by a multivariate ‘subordinate function value’ method, the overall ranking for antioxidant capacity of the nineteen species was as follows: C. chinensis>C. squarrosa>A. melilotoides>S. davidii>A. scaberrimus>B. ischcemum>S. bungeana>L. davurica>P. tanacetifolia>H. altaicus>C. lancifolia>H. rhamnoides>P. sepium>G. uralensis>A. scoparia>D. indicum>A. vestita>A. giraldii>B. alternifolia.

CLC Number:

Q948.11

HU Shu,JIAO Ju-ying,DU Hua-dong,MIAO Fang. Antioxidant properties of plants on different sites in the hilly-gullied Loess Plateau[J]. Acta Prataculturae Sinica, 2014, 23(5): 1-12.

References

Reference:

[1] Apel K, Hirt H. Reactive oxygen species: metabolism, oxidative stress, and signal transduction[J]. Annual Review of Plant Biology, 2004, 55: 373-399.

[2] Papadakis A K, Roubelakis-Angelakis K A. Polyamines inhibit NADPH oxidase-mediated superoxide generation and putrescine prevents programmed cell death induced by polyamine oxidase-generated hydrogen peroxide[J]. Planta, 2005, 220(6): 826-837. 

[3] Zhang J P. Plant Physiology[M]. Beijing: Higher Education Press, 2006.

[4] Li C Z, Zuo L P, Li Y, et al. Physiological responses in leaves of Reaumuria soongorica from different altitudes under osmotic stress[J]. Acta Prataculturae Sinica, 2013, 22(1): 176-182.

[5] Shan C J, Han R L, Liang Z S. Antioxidant properties of four native grasses in Loess Plateau under drought stress[J]. Acta Ecologica Sinica, 2012, 32(4): 1174-1184.

[6] Pei B, Zhang G C, Zhang S Y, et al. Effects of soil drought stress on photosynthetic characteristics and antioxidant enzyme activities in Hippophae rhamnoides Linn.seedings[J]. Acta Ecologica Sinica, 2013, 33(5): 1386-1396.

[7] Gechev T, Willekens H, Van Montagu M, et al. Different responses of tobacco antioxidant enzymes to light and chilling stress[J]. Journal of Plant Physiology, 2003, 160(5): 509-515.

[8] Carvalho L C. Solanum lycopersicon Mill. and Nicotiana benthamiana L. under high light show distinct responses to anti-oxidative stress[J]. Journal of Plant Physiology, 2008, 165: 1300-1312.

[9] Li H E, He J J, Qin Y, et al. The characteristics of storms and floods in the Yanhe River Basin and the flood forecasting scheme[J]. Hydrology, 2005, 25(5): 37-41.

[10] Chen Y M, Liang Y M, Cheng J M. The zonal character of vegetation construction on Loess Plateau[J]. Acta Phytoecologica Sinica, 2002, 26(3): 339-345.

[11] Wang L, Shao M A, Hou Q C. Preliminary research on measured indexes of dried soil layer[J]. Journal of Soil and Water Conservation, 2000, 14(4): 87-90.

[12] National Soil Survey Office. Second National Soil Survey Interim Technical Specification[M]. Beijing: Agricultural Press, 1979.

[13] Cai Y L, Song Y C. Adaptive ecology of Lian as in Tiantong Evergreen Broad-Leaved Forest, Zhejiang , ChinaI. leaf an atomical characters[J]. Acta Phytoecologica Sinica, 2001, 25(1): 90-98.

[14] Asada K. Production and scavenging of reactive oxygen species in chloroplasts and their function[J]. Plant Physiology, 2006, 141: 391-396.

[15] Wang H Z, Zhang J, Wu J Z, et al. Effect of different levels of nitrogen on physiological characteristics of flag leaves and grain yield of wheat[J]. Acta Prataculturae Sinica, 2013, 22(4): 69-75.

[16] Wu M J, Yu P. Physiological role of plant peroxidase[J]. Journal of Biology, 1994, (6): 14-16.

[17] Wu Z H, Zeng F H, Ma S J, et al. A review of advances in active oxygen metabolism in plants under water stress[J]. Subtropical Plant Science, 2004, 33(2): 77-80.

[18] Jimenez C. Differential reactivity of carotene isomers from Dunaliella bardawl toward oxygen radicals[J]. Plant Physiology, 1993, 101: 385.

[19] Sun Q, Hu J J. Plant Physiology Research Techniques[M]. Shaanxi: Northwest A & F University Press, 2006.

[20] Riffith O W. Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine[J]. Analytical Biochemistry, 1980, 106(1): 207-212.

[21] Zhang X Z. Determination of plant chlorophyll content - a mixture law of acetone and ethanol[J]. Liaoning Agricultural Science, 1986, (3): 26-28.

[22] Li Z, Peng F, Su X Y. Physiological responses of white clover by different leaf types associated with anti-oxidative enzyme protection and osmotic adjustment under drought stress[J]. Acta Prataculturae Sinica, 2013, 22(2): 257-263.

[23] Zhang Z L, Qu W J, Li X F. Plant Physiology Experimental Guidance[M]. Beijing: Higher Education Press, 2009.

[24] Liu X Y. Discussion soybean drought assessment method[J]. Journal of China Oil Crop, 1986, (4): 23-26.

[25] Liu F, Yang J, Zhang P J, et al. Relationships between geographical distribution of Artemisia giraldii and cli- mate[J]. Journal of Arid Land Resources and Environment, 2012, 26(6): 56-59.

[26] Tang X M, Wang W Q, Ma C Y. Physiological drought responses of Glycyrrhiza uralensis leaves under prolonged water stress[J]. Journal of Agricultural University of Hebei, 2008, 31(2): 16-20.

[27] Liu S W. Qinghai Flora (Volume 2)[M]. Qinghai: Qinghai People's Publishing House, 1999.

[28] Wu Z T, Zhang G Y. Effects of Abscisic Acid, Cytokinin and Malonaldehyde on Superoxide Dismutase activity[J]. Plant Physiology Communications, 1990, (4): 30-32.

[29] Li T, Sun J K, Tiao J Y, et al. Photosynthesis characteristics and Antioxidant Enzyme activity in Periploca sepium seedlings under drought stress[J]. Acta Botanica Boreali-Occidentalia Sinica, 2010, 30(12): 2466-2471.

[30] Zhu Z M, Yang C. Changes of four common plant populations growth and their anti-oxidative enzymatic system in desertification process[J]. Chinese Journal of Applied Ecology, 2004, 15(12): 2261-2266.

[31] Zeng Z H, Zhang Z Y. The oxidative damage of Mitochondrial DNA by free radicals and aging[J]. Progress in Biochemistry and Biophysics, 1995, (5): 429-432.

[32] Han G, Li S X, Xu P, et al. Analyisis of drought resistance on anatomical structure of leave of six species of shrubs[J]. Journal of Northwest Forestry University, 2006, 21(4): 43-46, 68.

[33] Zhang Y Y, He K N, Dong M, et al. Influences of water-stress on daily changes of LWP and water use efficiency of Shepherdia argentea and Hippophae rhamnoides[J]. Soil and Water Conservation in China, 2011, (6): 22-25.

[34] Bu C F, Liu G B, Dai Q H. Study on the effect of Sophora Viciifolia on the physical characteristics of soil[J]. Research of Soil and Water Conservation, 2003, 10(2): 25-27.

[35] Yang M H, Yao W Q, Cao M M, et al. Study on the activities of antioxidative enzymatic system of the common plant populations in desertification process on the southeast edge of Mu Us Sandy Land[J]. Journal of Northwest A & F University(Natural Science Edition), 2012, 40(7): 203-208.

[36] Hang F P, Dong L N, Luo W L, et al. Effects of stipa bungeana on soil water contents and nutrients of sloping lands in Loess Plateau of China[J]. Acta Agrestia Sinica, 2008, 16(4): 403-407.

[37] Guo Y, Han R L, Liang Z S. Effect of soil drought on growth and water use efficiency characteristics of four native gramineous grasses in Loess Plateau[J]. Acta Prataculturae Sinica, 2010, 19(2): 21-30.

[38] Chen S P, Bai Y F, Han X G. Variation of water-use efficiency of Leymus chinensis and Cleistogenes squarrosa in different plant communities in Xilin River Basin, Nei Mongol[J]. Acta Botanica Sinica, 2002, 12: 1484-1490.

[39] Wang Y. Study on water-consumption characteristics and drought-resistance mechanism of four Artermisia species in Loess Plateau[D]. Shaanxi: Northwest Agriculture and Forestry University, 2010.

参考文献：

[1] Apel K, Hirt H. Reactive oxygen species: metabolism, oxidative stress, and signal transduction[J]. Annual Review of Plant Biology, 2004, 55: 373-399.

[2] Papadakis A K, Roubelakis-Angelakis K A. Polyamines inhibit NADPH oxidase-mediated superoxide generation and putrescine prevents programmed cell death induced by polyamine oxidase-generated hydrogen peroxide[J]. Planta, 2005, 220(6): 826-837. 

[3] 张继澍. 植物生理学[M]. 北京: 高等教育出版社, 2006.

[4] 李朝周, 左丽萍, 李毅, 等. 两个海拔分布下红砂叶片对渗透胁迫的生理响应[J]. 草业学报, 2013, 22(1): 176-182.

[5] 单长卷, 韩蕊莲, 梁宗锁. 干旱胁迫下黄土高原4种乡土禾草抗氧化特性[J]. 生态学报, 2012, 32(4): 1174-1184.

[6] 裴斌, 张光灿, 张淑勇, 等. 土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响[J]. 生态学报, 2013, 33(5): 1386-1396.

[7] Gechev T, Willekens H, Van Montagu M, et al. Different responses of tobacco antioxidant enzymes to light and chilling stress[J]. Journal of Plant Physiology, 2003, 160(5): 509-515.

[8] Carvalho L C. Solanum lycopersicon Mill. and Nicotiana benthamiana L. under high light show distinct responses to anti-oxidative stress[J]. Journal of Plant Physiology, 2008, 165: 1300-1312.

[9] 李怀恩, 何娟娟, 秦毅, 等. 延河流域雨洪特性及洪水预报[J]. 水文, 2005, 25(5): 37-41.

[10] 陈云明, 梁一民, 程积民. 黄土高原林草植被建设的地带性特征[J]. 植物生态学报, 2002, 26(3): 339-345.

[11] 王力, 邵明安, 侯庆春. 土壤干层量化指标初探[J]. 水土保持学报, 2000, 14(4): 87-90.

[12] 全国土壤普查办公室. 全国第二次土壤普查暂行技术规程[M]. 北京:农业出版社, 1979.

[13] 蔡永立, 宋永昌. 浙江天童常绿阔叶林藤本植物的适应生态学I. 叶片解剖特征的比较[J]. 植物生态学报, 2001, 25(1): 90-98.

[14] Asada K. Production and scavenging of reactive oxygen species in chloroplasts and their function[J]. Plant Physiology, 2006, 141: 391-396.

[15] 王贺正, 张均, 吴金芝, 等. 不同氮素水平对小麦旗叶生理特性和产量的影响[J]. 草业学报, 2013, 22(4): 69-75.

[16] 吴明江, 于萍. 植物过氧化物酶的生理作用[J]. 生物学杂志, 1994, (6): 14-16.

[17] 吴志华, 曾富华, 马生健, 等. 水分胁迫下植物活性氧代谢研究进展(综述Ⅰ)[J]. 亚热带植物科学, 2004, 33(2): 77-80.

[18] Jimenez C. Differential reactivity of -carotene isomers from Dunaliella bardawl toward oxygen radicals[J]. Plant Physiology, 1993, 101: 385.

[19] 孙群, 胡景江. 植物生理学研究技术[M]. 陕西: 西北农林科技大学出版社, 2006.

[20] Riffith O W. Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine[J]. Analytical Biochemistry, 1980, 106(1): 207-212.

[21] 张宪政. 植物叶绿素含量测定——丙酮乙醇混合液法[J]. 辽宁农业科学, 1986, (3): 26-28.

[22] 李州, 彭燕, 苏星源. 不同叶型白三叶抗氧化保护及渗透调节生理对干旱胁迫的响应[J]. 草业学报, 2013, 22(2): 257-263.

[23] 张志良, 瞿伟菁, 李小方. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2009.

[24] 刘学义. 大豆抗旱性评定方法探讨[J]. 中国油料作物学报, 1986, (4): 23-26.

[25] 刘芳, 杨劼, 张璞进, 等. 茭蒿（Artemisia giraldii）的地理分布及与气候的关系[J]. 干旱区资源与环境, 2012, 26(6): 56-59.

[26] 唐晓敏, 王文全, 马春英. 长期水分胁迫下甘草叶片的抗旱生理响应[J]. 河北农业大学学报, 2008, 31(2): 16-20.

[27] 刘尚武. 青海植物志(第2卷)[M]. 青海：青海人民出版社, 1999.

[28] 伍泽堂, 张刚元. 脱落酸、细胞分裂素和丙二醛对超氧化物歧化酶活性的影响[J]. 植物生理学通讯, 1990, (4): 30-32.

[29] 李田, 孙景宽, 田家怡, 等. 干旱胁迫对杠柳光合特性及抗氧化酶活性的影响[J]. 西北植物学报, 2010, 30(12): 2466-2471.

[30] 朱志梅, 杨持. 沙漠化过程中四个共有种的生长和抗氧化系统酶类变化[J]. 应用生态学报, 2004, 15(12): 2261-2266.

[31] 曾昭惠, 张宗玉. 自由基对线粒体DNA的氧化损伤与衰老[J]. 生物化学与生物物理进展, 1995, (5): 429-432.

[32] 韩刚, 李少雄, 徐鹏, 等. 6种灌木叶片解剖结构的抗旱性分析[J]. 西北林学院学报, 2006, 21(4): 43-46, 68.

[33] 张益源, 贺康宁, 董梅, 等. 水分胁迫对银水牛果和沙棘叶水势日过程及水分利用效率的影响[J]. 中国水土保持, 2011, (6): 22-25.

[34] 卜崇峰, 刘国彬, 戴全厚. 纸坊沟流域狼牙刺对土壤物理性状的影响[J]. 水土保持研究, 2003, 10(2): 25-27.

[35] 杨梅焕, 姚顽强, 曹明明, 等. 毛乌素沙地东南缘沙漠化共有种植物叶片抗氧化酶活性研究[J]. 西北农林科技大学学报(自然科学版), 2012, 40(7): 203-208.

[36] 韩凤朋, 董丽娜, 罗文林, 等. 黄土高原侵蚀区长芒草对坡地土壤水分养分的影响[J]. 草地学报, 2008, 16(4): 403-407.

[37] 郭颖, 韩蕊莲, 梁宗锁. 土壤干旱对黄土高原4个乡土禾草生长及水分利用特性的影响[J]. 草业学报, 2010, 19(2): 21-30.

[38] 陈世苹, 白永飞, 韩兴国. 内蒙古锡林河流域不同植物群落中羊草和糙隐子草水分利用效率的变异(英文)[J]. Acta Botanica Sinica, 2002, 12: 1484-1490.

[39] 王勇. 黄土高原菊科蒿属四种植物的耗水规律及抗旱特性研究[D]. 陕西：西北农林科技大学, 2010.