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Acta Prataculturae Sinica ›› 2014, Vol. 23 ›› Issue (5): 1-12.DOI: 10.11686/cyxb20140501

• Orginal Article •     Next Articles

Antioxidant properties of plants on different sites in the hilly-gullied Loess Plateau

HU Shu1,JIAO Ju-ying2,DU Hua-dong2,MIAO Fang3   

  1. 1.College of Natural Resources and Environment, Northwest A&F University, Yangling 712100,China;
    2.Institute of Soil and Water Conservation, CAS&MWR, Yangling 712100, China;
    3.College of Life Sciences, Northwest A&F University, Yangling 712100, China
  • Received:2013-10-11 Online:2014-10-20 Published:2014-10-20

Abstract:

This study investigated antioxidant properties of main plant species on 5 different site classes (sunny or shady gully and inter-gully land or hilltops)) in the hilly-gullied Loess Plateau. From field sampling, the activities of the antioxidant enzymes-superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), the contents of two non-enzymatic antioxidants-reduced glutathione (GSH) and carotenoid (Car), and the malondialdehyde (MDA) levels of leaf tissues of nineteen species were determined. There were highly significant differences in MDA accumulation among species on each site (P<0.01). This indicated that the test species suffered varying degrees of membrane lipid peroxidation, with Buddleja alternifolia and Artemisia scoparia being highest and lowest respectively. There were significant differences in antioxidant enzyme activity or non-enzymatic antioxidant levels among sites for each test species, except for Bothriochloa ischcemum, Lespedeza davurica, Heteropappus altaicus and Sophora davidii, which suggests that these four species possessed upregulation of antioxidant enzymes and non-enzymatic antioxidant synthesis regardless of different site stresses, while Cleistogenes chinensis, Cleistogenes squarrosa, Stipa bungeana, Astragalus melilotoides, Astragalus scaberrimus, Glycyrrhiza uralensis, Potentilla tanacetifolia, A. scoparia, Artemisia vestita, Artemisia giraldii, Hippophae rhamnoides, Periploca sepium and B. alternifolia regulated their antioxidant enzymes or non-enzymatic antioxidant levels. From the botanical family perspective, antioxidant properties of the test species were distinct. CAT and SOD were respectively produced by five composite and five leguminous plant species to reduce damage from reactive oxygen species. Four graminaceous plants were able to attain high SOD, POD activities and Car contents to maintain a low level of membrane lipid peroxidation. Because of different carbon assimilation pathways, antioxidant properties of the test species were also distinct. In particular, C4 plants had higher GSH and Car contents than C3 plants. As assessed by a multivariate ‘subordinate function value’ method, the overall ranking for antioxidant capacity of the nineteen species was as follows: C. chinensis>C. squarrosa>A. melilotoides>S. davidii>A. scaberrimus>B. ischcemum>S. bungeana>L. davurica>P. tanacetifolia>H. altaicus>C. lancifolia>H. rhamnoides>P. sepium>G. uralensis>A. scoparia>D. indicum>A. vestita>A. giraldii>B. alternifolia.

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