The inoculant potential of plant growth promoting rhizobacteria strains to improve the yield and quality of Trifolium pratense cv. Minshan

doi:10.11686/cyxb20140527

Abstract

Abstract:

Interest in plant growth promoting rhizobacteria (PGPR) has increased due to their potential contribution to sustainable agricultural systems by reducing environmental impact, the consumption of non-renewable resources and input costs. This paper investigates the inoculant effect of PGPR on the hay yield and nutrient quality of Trifolium pratense cv. Minshan. Six PGPR and one rhizobia strains were identified by screening for phosphate solubilization, 3-indoleacetic acid (IAA) secretion and antagonism to plant pathogens. A PGPR inoculant was produced by mixing these strains and pot tests have been conducted to investigate its effects on the growth and quality of T. pratense cv. Minshan when combined with different amounts of chemical fertilizer. The results showed that the nitrogenase activity of the rhizobia strain was 488.2 C₂H₄ nmol/(mL·h). The phosphate solubilization and IAA secreting ability of the five PGPR strains was distributed across the ranges 50.34-229.5 mg/L and 0.86-12.01 μg/mL respectively. The PGPR compound bio-fertilizer demonstrated strong antagonism to the pathogens Fusarium oxysporium f. sp. cucumerinum, Rhizoctonia solani and Fusarium oxysporum f. niveum. The composite microbial inoculant complies with the quality standard for microbiological fertilizer in China (NY227-94). Applying the inoculant in combination with 75% of the control’s chemical fertilizers increased total hay yield by 4.76%. Nutrient quality also improved. The amount of crude protein, crude ash, calcium and phosphorus increased while the amount of neutral detergent fiber and acid detergent fiber declined. Applying the inoculant in combination with 50% of the control’s chemical fertilizers did not increase total hay yield, quality, or the isoflavone content of clover. The experiment has thus shown how the PGPR compound bio-fertilizer can be combined with chemical fertilizers to increase not only the yield and quality of T. pratense cv. Minshan but also the sustainability of agricultural systems.

CLC Number:

S541⁺.2

RONG Liang-yan,YAO Tuo,MA Wen-bin,LI De-ming,LI Ru-ren,ZHANG Jie,LU Sa. The inoculant potential of plant growth promoting rhizobacteria strains to improve the yield and quality of Trifolium pratense cv. Minshan[J]. Acta Prataculturae Sinica, 2014, 23(5): 231-240.

References

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