Welcome to Acta Prataculturae Sinica ! Today is Share:

Acta Prataculturae Sinica ›› 2014, Vol. 23 ›› Issue (5): 231-240.DOI: 10.11686/cyxb20140527

• Orginal Article • Previous Articles     Next Articles

The inoculant potential of plant growth promoting rhizobacteria strains to improve the yield and quality of Trifolium pratense cv. Minshan

RONG Liang-yan1,2,3,YAO Tuo1,2,MA Wen-bin1,2,LI De-ming3,LI Ru-ren4,ZHANG Jie3,LU Sa3   

  1. 1.Pratacultural College, Gansu Agricultural University, Lanzhou 730070, China;
    2.Key Laboratory of Grassland Ecosystem, Ministry of Education, Lanzhou 730070, China;
    3.Station of Grassland Technology Extension, Lanzhou 730046, China;
    4.Department of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2014-04-15 Online:2014-10-20 Published:2014-10-20

Abstract:

Interest in plant growth promoting rhizobacteria (PGPR) has increased due to their potential contribution to sustainable agricultural systems by reducing environmental impact, the consumption of non-renewable resources and input costs. This paper investigates the inoculant effect of PGPR on the hay yield and nutrient quality of Trifolium pratense cv. Minshan. Six PGPR and one rhizobia strains were identified by screening for phosphate solubilization, 3-indoleacetic acid (IAA) secretion and antagonism to plant pathogens. A PGPR inoculant was produced by mixing these strains and pot tests have been conducted to investigate its effects on the growth and quality of T. pratense cv. Minshan when combined with different amounts of chemical fertilizer. The results showed that the nitrogenase activity of the rhizobia strain was 488.2 C2H4 nmol/(mL·h). The phosphate solubilization and IAA secreting ability of the five PGPR strains was distributed across the ranges 50.34-229.5 mg/L and 0.86-12.01 μg/mL respectively. The PGPR compound bio-fertilizer demonstrated strong antagonism to the pathogens Fusarium oxysporium f. sp. cucumerinum, Rhizoctonia solani and Fusarium oxysporum f. niveum. The composite microbial inoculant complies with the quality standard for microbiological fertilizer in China (NY227-94). Applying the inoculant in combination with 75% of the control’s chemical fertilizers increased total hay yield by 4.76%. Nutrient quality also improved. The amount of crude protein, crude ash, calcium and phosphorus increased while the amount of neutral detergent fiber and acid detergent fiber declined. Applying the inoculant in combination with 50% of the control’s chemical fertilizers did not increase total hay yield, quality, or the isoflavone content of clover. The experiment has thus shown how the PGPR compound bio-fertilizer can be combined with chemical fertilizers to increase not only the yield and quality of T. pratense cv. Minshan but also the sustainability of agricultural systems.

CLC Number: