Exogenous nitric oxide elevated alkali tolerance of Avena nuda seedlings

doi:10.11686/cyxb2014352

Abstract

Abstract: The effects of sodium nitroprusside (SNP) as a nitric oxide (NO) donor on growth, leaf reactive oxygen metabolism and osmotic accumulation in naked oat (Avena nuda) seedlings under NaHCO₃ stress were investigated by a sand nutrient cultivation method. SNP exposure at concentration ranges of 1-200 μmol/L, especially at 25 μmol/L, significantly alleviated NaHCO₃-related growth inhibition of seedlings by decreasing the levels of O2·-, H₂O₂, malondialdehyde and organic acid, and increased the activities of superoxide dismutase, catalase, peroxidase, ascorbate peroxidase and plasma membrane H⁺-ATPase in leaves under 75 mmol/L NaHCO₃ stress. Similarly, the concentrations of reduced glutathione, soluble sugar, soluble protein, free amino acid and proline as well as K⁺/Na⁺ ratio were also increased significantly in seedling leaves under the same stress, but ascorbic acid concentration was not affected significantly. The results indicate that NO may alleviate the damage and growth inhibition caused by alkali stress in naked oat seedlings via activation of antioxidant system activity, acceleration of osmotic accumulation and maintenance of Na⁺ and K⁺ balance, thus improving alkali resistance of the naked oat plants.

LIU Jian-Xin, WANG Jin-Cheng, WANG Rui-Juan, JIA Hai-Yan. Exogenous nitric oxide elevated alkali tolerance of Avena nuda seedlings[J]. Acta Prataculturae Sinica, 2015, 24(8): 110-117.

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