Effects of gibberellic acid on forage quality of rice (Oryza sativa) straw

doi:10.11686/cyxb2016014

Abstract

Abstract: In this study, two different rice (Oryza sativa) varieties, Liangyoupeijiu (late maturing indica) and Nanjing 44 (early maturing japonica), were used to investigate the mechanisms of gibberellic acid (GA) regulation of the forage value of rice straw during late growth. GA was applied 15 days after anthesis at a rate of 45 g/ha. The endogenous plant hormones [such as GA, 3-indole acetic acid (IAA), and abscisic acid (ABA)], superoxide dismutase (SOD) and peroxidase (POD) activities, and malondialdehyde (MDA) content in the first top leaf were assessed on the 3rd d and 14th d after GA application. Forage quality and fermentation quality of rice straw were also assessed after rice harvest. The results indicated that the forage value Liangyoupeijiu and Nanjing 44 were significantly improved by GA application as a result of reduced senescence. However, there were significant cultivar differences; the green leaf area per tiller of Liangyoupeijiu was significantly higher than that of Nanjing 44 (P<0.01); nonstructural carbohydrates (NSC) and crude protein (CP) content in the straw of Liangyoupeijiu were significantly higher than those of Nanjing 44 (P<0.05). The MDA content of GA treated Liangyoupeijiu was significantly lower than that of the control (P<0.05), while the SOD and POD activities of GA treated plants were significantly higher than those of the control (P<0.05). The GA content and the GA∶ABA ratio of GA treated plants of Liangyoupeiju were significantly higher than those of the control (P<0.05) and Nanjing 44. It was concluded that application of GA during late growth could significantly improve the forage quality of rice straw. The effects of GA were greater in Liangyoupeijiu than in Nanjing 44.

DONG Chen-Fei, GU Hong-Ru, DING Cheng-Long, XU Neng-Xiang, ZHANG Wen-Jie. Effects of gibberellic acid on forage quality of rice (Oryza sativa) straw[J]. Acta Prataculturae Sinica, 2016, 25(11): 94-102.

References

[1] Li J, Shen Y X, Cai Y M. Improvement of fermentation quality of rice straw silage by application of a bacterial inoculant and glucose. Asian-Australian Journal of Animal Science, 2010, 23(7): 901-906.
[2] Ma Y Y, Li Y F, Cheng Y F, et al . Effects of different chemical treatments on fermentation characteristics of rice straw in vitro . Acta Prataculturae Sinica, 2014, 23(3): 350-355.
[3] Dong C F, Gu H R, Ding C L, et al . Effects of gibberellic acid application after anthesis on the feeding value of double-purpose rice ( Oryza sativa L.) straw at harvest. Field Crops Research, 2012, 131: 75-80.
[4] Dong C F, Gu H R, Xu N X, et al . Effects of gibberellic acid on the nonstructural carbohydrates content in rice ( Oryza sativa L) straw harvested at different times. Acta Prataculturae Sinica, 2015, 24(8): 53-64.
[5] Kende H, Zeevaart J A D. The five “classical” plant hormones. Plant Cell, 1997, 9: 1197-1210.
[6] Brenner M L, Cheikh N. The Role of Hormones in Photosynthate Partitioning and Seed Filling[M]//Davies P J. Plant Hormones, Physiology, Biochemistry and Molecular Biology. The Netherlands: Kluwer Academic Publishers, 1995: 649-670.
[7] Davies P J. Introduction[M]//Davies P J. Plant Hormones, Biosynthesis, Signal Transduction, Action. The Netherlands: Kluwer Academic Publishers, 2004: 1-35.
[8] Wen F, Zhang Z, Bai T, et al . Proteomics reveals the effects of gibberellic acid (GA 3 ) on salt-stressed rice ( Oryza sativa L.) shoots. Plant Science, 2010, 178: 170-175.
[9] Konishi H, Maeshima M, Komatsu S. Characterization of vacuolar membrane proteins changed in rice root treated with gibberellin. Journal of Proteome Research, 2005, 4: 1775-1780.
[10] Komatsu S, Konishi H. Proteome analysis of rice root proteins regulated by gibberellin. Genomics Proteomics Bioinformatics, 2005, 3: 132-142.
[11] Shen S, Sharma A, Komatsu S. Characterization of proteins responsive to gibberellin in the leaf-sheath of rice ( Oryza sativa L.) seedling using proteome analysis. Biological Pharmaceutical Bulletin, 2003, 26: 129-136.
[12] Komatsu S, Zang X, Tanaka N. Comparison of two proteomics techniques used to identify proteins regulated by gibberellin in rice. Journal of Proteome Research, 2006, 5: 270-276.
[13] Huang D, Wang S, Zhang B, et al . A gibberellin-mediated DELLA-NAC signaling cascade regulates cellulose synthesis in rice. The Plant Cell, 2015, 27: 1681-1696.
[14] Yoshida S. Laboratory Manual for Physiological Studies of Rice[M]. Philippines: Los Baios, IRRI, 1976: 43.
[15] Van Soest P J, Robertson J B, Lewis B A. Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 1991, 74: 3583-3597.
[16] Goto I, Minson D J. Prediction of the dry matter digestibility of tropical grasses using a pepsin-cellulase essay. Animal Feed Science and Technology, 1977, 2(3): 247-253.
[17] Barker S B, Summerson W H. The colorimetric determination of lactic acid in biological material. Journal of Biology Chemistry, 1941, 138: 535-554.
[18] Weather M W. Phenol-hypochlorite reaction for determinations of ammonia. Annual of Chemistry, 1967, 39: 971-974.
[19] Dong C F, Liu X B, Qu H, et al . Dynamical partition of photosynthates in tillers of rice ( Oryza sativa L.) during late growth period and its correlation with feeding value of rice straw at harvest. Field Crops Research, 2011, 123(3): 273-280.
[20] Guo Y P, Ren Y X, Liu G H, et al . Effects of calcium (CaCl 2 ), GA 3 and complex liquid on the physiological characteristics of alfalfa seedlings under drought stress. Acta Prataculturae Sinica, 2015, 24(7): 89-96.
[21] Chen X B, Liao A J, Luo Z M. Physiological properties of the roots and leaves of a high yield rice line at the late growth stage. Life Science Research, 1999, 3(3): 250-255.
[22] Liu K. Regulation of Abscisic Acid and Ethylene to Grain Filling in Rice and Wheat and Its Physiological Mechanism[D]. Yangzhou: Yangzhou University, 2008.
[23] Wang A G, Luo G H, Shao C B. Plant oxygen metabolism and cell damage induced by reactive oxygen species. Collected Papers of China Academy of Sciences, Southern China Institute of Botany, 1989, 5: 11-23.
[24] Chen S Y. Injury of membrane lipid peroxidation to plant cell. Plant Physiology Communications, 1991, 27(2): 84-90.
[25] Davies P J. Introduction[M]//Davies P J. Plant Hormones, Physiology, Biochemistry and Molecular Biology. The Netherlands: Kluwer Academic Publishers, 1995: 1-12.
[2] 马艳艳, 李袁飞, 成艳芬, 等. 不同化学处理对稻草体外发酵动态变化的影响. 草业学报, 2014, 23(3): 350-355.
[4] 董臣飞, 顾洪如, 许能祥, 等. 赤霉素对不同收获时间的稻草中非结构性碳水化合物含量的影响. 草业学报, 2015, 24(8): 53-64.
[20] 郭郁频, 任永霞, 刘贵河, 等. 外源钙和赤霉素对干旱胁迫下苜蓿幼苗生理特性的影响. 草业学报, 2015, 24(7): 89-96.
[21] 陈信波, 廖爱君, 罗泽民. 大穗型水稻生育后期叶片和根系生理的特性. 生命科学研究, 1999, 3(3): 250-255.
[22] 刘凯. 脱落酸和乙烯对水稻与小麦籽粒灌浆的调控作用及其机理[D]. 扬州: 扬州大学, 2008.
[23] 王爱国, 罗广华, 邵从本. 活性氧引起的植物氧代谢和细胞伤害. 中国科学院华南植物研究所集刊, 1989, 5: 11-23.
[24] 陈少峪. 膜脂过氧化对植物细胞的伤害. 植物生理学通讯, 1991, 27(2): 84-90.