Effects of rhizosphere soil extraction from alfalfa-corn and alfalfa-wheat fields on alfalfa seed germination and seedling physiology and growth

doi:10.11686/cyxb2017401

Abstract

Abstract: To provide references for the study of alfalfa-corn and alfalfa-wheat rotation systems, the allelopathic effects of rhizosphere soil extraction from alfalfa-corn and alfalfa-wheat fields on the seed germination, seedling physiology and growth indexes of Medicago sativa cv. Gannong No.3 were investigated. Soil extraction from a five-year alfalfa field served as control (CK). Four rotation treatments were investigated: five-year alfalfa-corn (AC), five-year alfalfa-wheat (AW), five-year alfalfa-corn-corn (ACC), and five-year alfalfa-wheat-wheat (AWW). Three soil extraction concentrations (10, 50 and 160 g·L^-1) were applied and seed germination and seedling growth rates were determined. The results showed that: 1) Seed germination percentages for all the rotation treatments under the three concentrations increased significantly compared with CK, with allelopathic indexes exceeding zero. Seed germination percentages under treatment AWW with soil extraction concentrations of 10, 50 and 160 g·L^-1were the highest at 29.22%, 68.09% and 54.24% more than CK respectively (P<0.05). There were also significant differences between AWW and the other rotation treatments. The germ and radical lengths of each treatment except for ACC increased as soil extraction concentrations went up and were significantly higher than that of CK (P<0.05). Under the same concentration, the germ length of treatment AWW significantly increased compared with AC, ACC, AW, and the radical length was significantly higher than that of AC and AW (P<0.05). However, the radical length of AWW was not significantly larger than that of ACC (P>0.05). 2) The soluble sugar content and superoxide dismutase activity of alfalfa seedlings in each rotation treatment were significantly enhanced with increasing soil extraction concentrations (P<0.05). The malondialdehyde content and catalase activity in treatments AC and ACC were significantly promoted, while in treatment AW and AWW the catalase activity weakened significantly (P<0.05) and the malondialdehyde content decreased at first and then increased.

Key words: rotation, soil extracts, seed germination, physiology characteristic

YIN Guo-li, WU Fang, TAO Rong, SHI Shang-li, CAI Zhuo-shan. Effects of rhizosphere soil extraction from alfalfa-corn and alfalfa-wheat fields on alfalfa seed germination and seedling physiology and growth[J]. Acta Prataculturae Sinica, 2018, 27(5): 153-161.

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