Research on exogenous NO and reverse regulation to the dynamic changes of antioxidant enzymes and isoenzymes in alfalfa seed germination under PEG stress

doi:10.11686/cyxb2017478

Abstract

Abstract: To study the effects of exogenous NO and reverse regulation antioxidant enzyme activities and isoenzymes of alfalfa under PEG stress, alfalfa seeds were used as experimental materials, they were pretreated with nitric oxide donor sodium nitroprusside (SNP) or nitric oxide scavenger c-PTIO, the methods of spectrophotometry and isoenzyme electrophoresis were used to analyze the activities of antioxidant enzymes. The results showed that: under the PEG stress, the external application of 0.1 mmol·L^-1 SNP, the activities of POD were 32.72% lower than the PEG treatment on the 6th day; the activities of SOD and CAT were 10.48% and 23.60% higher than the PEG treatment on the 4th day respectively, so they could alleviate the oxidation damage on alfalfa seeds which caused by PEG stress. In addition, the exogenous of c-PTIO under drought stress inhibited the activity of antioxidant system during alfalfa germination stage. According to the number and intensity of isozymes, with prolonging the PEG stress time, the bands of POD isoenzymes activity increased, and only one strip on the second day, while 9 strips on the 4th day; the expression of isoenzymes of SOD and CAT were not significantly, but the strength of the enzyme band changed to some extent, the expression of S3 enzyme band was gradually weakened with prolonged the treatment time; CAT isoenzyme band had been kept at 2 bands all through, showed no significantly changes in strength. Therefore, exogenous NO rapidly responded to antioxidant enzymes during the germination of alfalfa under PEG stress and played an important protective role in the maintenance of oxygen free radical metabolism.

Key words: nitric oxide, PEG stress, Medicago sativa, antioxidant enzymes, isozyme electrophoresis

XIN Xia-qing, WEI Xiao-hong, HAN Ting, YUE Kai, ZHAO Ying. Research on exogenous NO and reverse regulation to the dynamic changes of antioxidant enzymes and isoenzymes in alfalfa seed germination under PEG stress[J]. Acta Prataculturae Sinica, 2018, 27(10): 105-112.

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