Research on genetic and phenotypic differences of Pennisetum purpureum mutants generated by irradiation

doi:10.11686/cyxb2018006

Abstract

Abstract: In order to provide a theoretical basis for mutation-induced breeding of Pennisetum purpureum, stems of P. purpureum were irradiated with ⁶⁰Co-γ rays at differing doses of 10, 20 and 30 Gy. Then, we screened the resulting mutants and defined their phenotypic variation, and identified the genetic differences at a molecular level, using SSR. The irradiated plants showed a tendency to dwarfism. The number of tillers, stems and the length of leaves were the traits most sensitive to radiation and most easily mutated. Among all the mutants, two designated F30-39 and F30-41 were significantly shortened plants, and were determined by cluster analysis to belong to a group which had the greatest genetic difference from the control material. The genetic similarity coefficient between controls and “30 Gy” mutants ranged from 0.3793 to 0.9655, with the average being 0.8276, while the number of mutation sites ranged between 4 and 66, with an average of 19.3. Both indices were greater than those of “10 and 20 Gy” mutants. Considering the survival rate and genetic mutation frequency of irradiated plants, 30 Gy was selected as the most suitable radiation dose level for mutagenesis.

Key words: Pennisetum purpureum, radiation induction of mutation, genetic variation, phenotypic variation, SSR molecular markers

WU Bing-chao, ZHANG Huan, TONG Lei, DU Zhao-chang, HU Jia-ling, CHEN Yi, ZHANG Xin-quan, LIU Wei, HUANG Lin-kai. Research on genetic and phenotypic differences of Pennisetum purpureum mutants generated by irradiation[J]. Acta Prataculturae Sinica, 2018, 27(11): 77-86.

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