Difference in soil microbial diversity in artificial grasslands of the Northwest Plateau of Sichuan Province

doi:10.11686/cyxb2018489

Abstract

Abstract: In order to determine the influence of sown species in artificial grassland on soil microbial diversity in the Northwest Plateau of Sichuan province, an experiment was set up comparing stands of Medicago sativa (Ms), Avena sativa (As), and natural grassland (NG). The microbial community composition and structure of soil samples from the three vegetation types were analyzed by using Illumina Hiseq high-throughput sequencing technology. The results showed: 1) There was no significant difference among the three vegetation types with respect to the abundance and diversity of bacterial communities isolated from soil samples. The fungal community was nearly the same for As and NG, while Ms had higher fungal diversity than the other two vegetation types. 2) The dominant phyla of bacteria for the three samples were Proteobacteria, Acidobacteria, Actinobacteria and Verrucomicrobia. Acidobacteria were less abundant in the As soil than in the NG soil (P<0.05), whereas Actinobacteria had higher relative abundance in the As soil than in the NG soil (P<0.05). At the class level, Spartobacteria, α-proteobacteria, β-proteobacteria, Thermoleophilia, Acidobacteria and Actinobacteria were the predominant bacterial communities, and their relative abundance differed significantly among the three vegetation types (P<0.05). 3) The dominant fungal phyla were Ascomycota, Zygomycota, and Basidiomycota. The dominant taxonomic classes were Zygomycotes, Sordariomycetes, Agaricomycetes, Leotiomycetes, and Dothideomycetes, and the relative abundance of these dominant classes also differed significantly among the three vegetation types. 4) Beta-diversity analyses showed that the structure of soil fungal communities were significantly different among the three vegetation types, and soil microbial diversity coefficients for NG were greater than those for Ms and As. A cluster analysis showed that restoration by artificial grassland increased the relative abundance of beneficial microbes, but the relative abundance of soil plant pathogenic fungi was also significantly increased.

Key words: Northwest Sichuan alpine pastoral regions, artificial grassland, high-throughput sequencing, microbial diversity

WU Wen-xian, ZHANG Lei, HUANG Xiao-qin, YANG Xiao-xiang, XUE Long-hai, LIU Yong. Difference in soil microbial diversity in artificial grasslands of the Northwest Plateau of Sichuan Province[J]. Acta Prataculturae Sinica, 2019, 28(3): 29-41.

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