Effects of exogenous mannose application on drought tolerance, sugars, and sugar alcohol accumulation in white clover

doi:10.11686/cyxb2019042

Abstract

Abstract: Effects of exogenous application of mannose on water status, osmotic adjustment, antioxidant capacity, photosynthetic response, sugars, and sugar alcohol accumulation in ‘Ladino’ white clover were studied under drought stress using physiological analysis and gas chromatography-mass spectrometer (GC-MS) technology. Results showed that drought stress significantly decreased relative water content, chlorophyll content, photochemical efficiency (F_v/F_m), and net photosynthesis rate resulting in membrane lipid peroxidation and decrease in cell membrane stability in leaves of white clover. However, white clover improved water use efficiency and decreased osmotic potential in order to acclimate to drought stress. The application of exogenous mannose (30 mmol·L^-1) significantly increased photosynthesis, total antioxidant capacity, and osmotic adjustment, thereby alleviating drought-induced oxidative damage and maintaining better water balance during drought stress. GC-MS analysis showed that drought stress induced differential changes of many sugars and sugar alcohols in leaves of white clover. Exogenous mannose application enhanced the accumulation of many sugars (mannose, glucose, fructose, galactose, xylose, sucrose, maltose, and trehalose) and sugar alcohols (myo-inositol, glycerol, and D-pinitol) in white clover under drought stress contributing to improved osmotic adjustment and energy supply for the maintenance of growth.

Key words: osmotic adjustment, water stress, antioxidant, photosynthesis, drought tolerance, metabolic regulation

LI Zhou, PENG Yan, YIN Shu-xia, HAN Lie-bao. Effects of exogenous mannose application on drought tolerance, sugars, and sugar alcohol accumulation in white clover[J]. Acta Prataculturae Sinica, 2019, 28(12): 85-93.

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