Selection of different explants from hops and establishment of a regeneration system

doi:10.11686/cyxb2019160

Abstract

Abstract: The aim of this research was to study the effects of different explants and hormone combinations on callus induction, differentiation and regeneration of hops, and finally to establish a stable and efficient hop plant regeneration system. Five different hop genotypes with semi-lignified shoots were selected, and the optimal explant donors were screened by cutting rooting and germination. The root tip, axillary bud and leaves of the hop genotypes were used as explants to study the effects of different exogenous hormone combinations on callus induction, adventitious bud differentiation and rooting. It was found that a genotype ‘PJ274’ had a rooting rate of up to 85% under the test growth conditions, and the germination rate of PJ274 material in hydroponic liquid of the ‘Japanese garden’ formula was as high as 40%, making PJ274 the most suitable among the tested genotypes for producing hop explants. Comparing the root tip, axillary bud and leaf as tissues for explant-induced callus, we found that axillary bud to be optimal with an induction rate of 51.11 %. The optimum culture conditions for callus induction using hop axillary buds were: 6-Benzylaminopurine (6-BA) 0.1 mg·L^-1+IAA (indole-3-acetic acid) 1.0 mg·L^-1+2,4-dichlorophenoxyacetic acid (2,4-D) 2.0 mg·L^-1, and with this formulation callus induction rate reached 86.67%. The differentiation rate of axillary bud callus was achieved using 6-BA 1.0 mg·L^-1+1-naphthaleneacetic acid) 0.2 mg·L^-1+2,4-D 2.0 mg·L^-1, with up to 66.67% success. The optimal hormoned regime for promotion of callus root differentiation of hops was 6-BA 0.1 mg·L^-1+indole-3-butytric acid 1.0 mg·L^-1, with a maximum rooting rate of 66.67% achieved. The test tube seedlings were transplanted after refining, and the survival rate was 80%. With these protocols, a high-output regeneration system for hops, using axillary buds as explants, was successfully established, so laying a foundation for the preservation of hop germplasm resources, tissue culture and rapid propagation, and genetically engineered breeding.

Key words: Humulus lupulus, explant, callus, regeneration system, hormone

YANG Ke, CHEN Yi-you, WANG Jun-cheng, YAO Li-rong, MENG Ya-xiong, MA Xiao-le, LI Bao-chun, SI Er-jing, WANG Hua-jun. Selection of different explants from hops and establishment of a regeneration system[J]. Acta Prataculturae Sinica, 2019, 28(8): 209-217.

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