Effects of different phosphorus supply levels on morphology and physiology of Paspalum wettsteinii

doi:10.11686/cyxb2019471

Abstract

Abstract: This research explored the morphological and physiological changes of Paspalum wettsteinii under different phosphorus supply levels, to understand the adaptation strategies to low phosphorus stress, and to provide a theoretical foundation for understanding phosphorus nutrient management and physiological regulation in P. wettsteinii. The experiment was carried out using a sand culture method, and treatments comprised 5 levels of phosphorus: 2, 20, 200, 600 and 1000 μmol·L^-1, with 200 μmol·L^-1 regarded as the control. The changes in seedling growth, root morphology and protective enzyme activity were measured at 10, 20 and 30 days after the imposition of stress. It was found that under low phosphorus supply, the height and aboveground biomass were significantly decreased (P<0.05), with leaf area and leaf perimeter also showing a decreasing trend. Meanwhile, the root biomass, total root length, root surface area, root volume, number of root tips and root∶shoot ratio were all higher at the low phosphorus supply than in the control. Superoxide dismutase, peroxidase (POD), catalase (CAT) and acid phosphatase (ACP) activities were significantly enhanced (P<0.05), and leaf lamina and root conductivity were significantly improved (P<0.05) under low phosphorus supply levels. Principal component analysis showed that CAT activity, POD activity, root acid phosphatase activity, number of root tips and root membrane permeability were significantly affected by phosphorus stress, and better performance when the phosphorus supply level is 600 μmol·L^-1 than others. At the phosphorus supply level of 200 μmol·L^-1, the multi-trait performance of P. wettsteinii was ranked second, and was exceeded only at the high phosphorus level. Partial least squares regression analysis was used to analyze the relationship between each index parameter and plant biomass under different phosphorus supply levels of P. wettsteinii, and it was concluded that root surface area, root acid phosphatase activity (ACP) and leaf perimeter were crucial indexes affecting the biomass of P. wettsteinii. In summary, these results show that P. wettsteinii adapted to low-phosphorus stress through reducing above-ground biomass, increasing root weight and root surface area, and promoting protective enzyme activity.

Key words: Paspalum wellsteinii, phosphorus stress, root morphology, physiological property, biomass

SUN Xiao-fu, HUANG Li-juan, WANG Pu-chang, ZHAO Li-li, LIU Fang. Effects of different phosphorus supply levels on morphology and physiology of Paspalum wettsteinii[J]. Acta Prataculturae Sinica, 2020, 29(8): 58-69.

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