Effects of resveratrol supplementation in the perinatal diet on production performance， blood indexes， and transcript abundance of genes encoding inflammatory factors in goats

doi:10.11686/cyxb2023190

Abstract

Abstract:

The aim of this experiment was to study the effects of resveratrol in the perinatal diet of goats in terms of growth performance， blood indexes， and the transcript levels of genes encoding inflammatory factors. Eighteen multiparous Subai female goats at 129 days of gestation （21 days prior to expected parturition） were divided into a control group （basal diet） and a Res group （supplemented with resveratrol at 400 mg·kg^-1 dry matter intake）. Blood was collected 15 days before （d_-15） and 0 （d₀） and 15 （d₁₅） days after parturition， and the productive performance， blood indexes， and transcript levels of genes encoding inflammatory factors were determined. The pre-test period was 1 week and the formal test period was 4 weeks. It was found that the Res treatment did not significantly affect ewe feed intake， lamb birth weight， body size， or daily weight gain. The feed intake significantly changed between before and after parturition （P=0.023）， and ewes in both groups had the lowest feed intake on the day of parturition. In terms of biochemical indexes， the Res treatment significantly affected d₀ non-etherized fatty acid levels， and the time×Res interaction was significant （P<0.05）. The Res treatment tended to affect total cholesterol （TC）（P=0.093）， but did not significantly affect triglyceride levels. In both groups， the triglyceride levels differed significantly between before and after delivery （P=0.002）. The Res treatment did not significantly affect high density lipoprotein levels， but there was a trend towards an effect of the Res×time interaction （P=0.055）. Neither low density lipoprotein nor glucose levels showed significant changes （P>0.05）. In terms of antioxidant indexes， the Res treatment significantly increased superoxide dismutase activity on d₁₅ （P<0.05）， and there was a tendency for Res treatment to affect the total antioxidant capacity （P=0.078）. However， the Res treatment did not significantly affect glutathione peroxidase activity or the malondialdehyde content （P>0.05）. In terms of immunity indexes， the Res treatment significantly affected the immunoglobulin A level on d₀ （P<0.05） and the immunoglobulin M levels on d₀ and d₁₅ （P<0.05）， but there was no significant change in the immunoglobulin G level during the test period （P>0.05）. The Res treatment led to significantly decreased transcript levels of TNFα and IFNγ on d₁₅ （P<0.05）， but there were no significant changes in the transcript levels of Nrf2， HO-1， IL-1， and IL-6 （P>0.05）. Our results show that addition of resveratrol to the diet of perinatal ewes affects free fatty acid levels in the blood， enhances their antioxidant performance， and reduces their inflammatory response to a certain extent.

Key words: perinatal period, goats, resveratrol, production performance, inflammatory factor

Jie ZHAO, Heng-guang CHEN, Xiao-meng PEI, Hao YU, Yin-ying XU, Da-gan MAO. Effects of resveratrol supplementation in the perinatal diet on production performance， blood indexes， and transcript abundance of genes encoding inflammatory factors in goats[J]. Acta Prataculturae Sinica, 2024, 33(4): 210-220.

Figures/Tables 9

References 52

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原料组成 Diet ingredients	产前日粮 Prepartum diet （%）	产后日粮 Postpartum diet （%）	营养水平 Nutrition level	产前日粮 Prepartum diet	产后日粮 Postpartum diet
玉米Corn grain	27.78	30.50	消化能DE （MJ·kg ^-1）	14.17	14.66
麸皮Wheat bran	16.67	5.00	粗蛋白CP （%）	14.89	17.69
豆粕Soybean meal	5.56	10.00	酸性洗涤纤维ADF （%）	24.22	21.93
预混料Premix	2.78	2.50	中性洗涤纤维NDF （%）	31.03	36.25
食盐NaCl	0.56	0.25	粗脂肪EE （%）	3.79	3.88
蒙脱石Montmorillonite	0.11	0.50	粗灰分Ash （%）	4.84	5.26
鱼肝油Cod liver oil	0.28	0.12	钙Ca （%）	0.50	0.65
磷酸氢钙CaHPO₄	0.43	0.50	磷P （%）	0.38	0.39
小苏打NaHCO₃	1.11	0.50
维生素E Vitamin E	0.28	0.13
青贮玉米Silage corn	35.55	40.00
花生秧Peanut seedling	8.89	10.00
合计Total	100.00	100.00

原料组成 Diet ingredients	产前日粮 Prepartum diet （%）	产后日粮 Postpartum diet （%）	营养水平 Nutrition level	产前日粮 Prepartum diet	产后日粮 Postpartum diet
玉米Corn grain	27.78	30.50	消化能DE （MJ·kg ^-1）	14.17	14.66
麸皮Wheat bran	16.67	5.00	粗蛋白CP （%）	14.89	17.69
豆粕Soybean meal	5.56	10.00	酸性洗涤纤维ADF （%）	24.22	21.93
预混料Premix	2.78	2.50	中性洗涤纤维NDF （%）	31.03	36.25
食盐NaCl	0.56	0.25	粗脂肪EE （%）	3.79	3.88
蒙脱石Montmorillonite	0.11	0.50	粗灰分Ash （%）	4.84	5.26
鱼肝油Cod liver oil	0.28	0.12	钙Ca （%）	0.50	0.65
磷酸氢钙CaHPO₄	0.43	0.50	磷P （%）	0.38	0.39
小苏打NaHCO₃	1.11	0.50
维生素E Vitamin E	0.28	0.13
青贮玉米Silage corn	35.55	40.00
花生秧Peanut seedling	8.89	10.00
合计Total	100.00	100.00

基因 Gene	序列号 Genebank number	引物序列 Sequence of nucleotide （5′-3′）	产物长度 Product length （bp）
IL-1β	XM_013967700.2	F： AATGAGCCGAGAAGTGGTGTT； R： TACCCAAGGCCACAGGAATCT	80
TNFα	NM_001286442.1	F： CTGGTTCAGACACTCAGGTCA； R： GAGGGCATTGGCATACGAG	120
HO-1	NM_001285567.1	F： AGCAAGGCACAAGACTCGG； R： ACATTGCGTAAAGGCCCACA	142
IFNγ	NM_001285682.1	F： GCAGATCCAGCGCAAAGCCAT； R： TTGATGCTCTCCGGCCTCGAA	119
IL-6	NM_001285640.1	F： CAGCAAGGAGACACTGGCAGAA； R： AGCAAATCGCCTGATTGAACCC	95
Nrf2	NM_001314327.1	F： TGACAATGAGGTTTCTTCG； R： GTGGCTACCTGAACGAACA	129
GAPDH	XM_005680968.3	F： CCGTTCGACAGATAGCCGTAA； R： AGGATCTCGCTCCTGGAAGAT	296

基因 Gene	序列号 Genebank number	引物序列 Sequence of nucleotide （5′-3′）	产物长度 Product length （bp）
IL-1β	XM_013967700.2	F： AATGAGCCGAGAAGTGGTGTT； R： TACCCAAGGCCACAGGAATCT	80
TNFα	NM_001286442.1	F： CTGGTTCAGACACTCAGGTCA； R： GAGGGCATTGGCATACGAG	120
HO-1	NM_001285567.1	F： AGCAAGGCACAAGACTCGG； R： ACATTGCGTAAAGGCCCACA	142
IFNγ	NM_001285682.1	F： GCAGATCCAGCGCAAAGCCAT； R： TTGATGCTCTCCGGCCTCGAA	119
IL-6	NM_001285640.1	F： CAGCAAGGAGACACTGGCAGAA； R： AGCAAATCGCCTGATTGAACCC	95
Nrf2	NM_001314327.1	F： TGACAATGAGGTTTCTTCG； R： GTGGCTACCTGAACGAACA	129
GAPDH	XM_005680968.3	F： CCGTTCGACAGATAGCCGTAA； R： AGGATCTCGCTCCTGGAAGAT	296

采样时间 Sampling time	对照组 Con （kg）	白藜芦醇组 Res （kg）	P值P value
采样时间 Sampling time	对照组 Con （kg）	白藜芦醇组 Res （kg）	组别Group （G）	时间Time （T）	G×T
d_-15	1.46±0.11	1.48±0.14	0.207	0.023	0.877
d₀	1.39±0.09	1.40±0.09
d₁₅	1.50±0.11	1.54±0.08