Identification of SRAP molecular markers linked to salt tolerance in Zoysia grasses

Abstract

Abstract: In this paper, bulked segregant analysis was used to identify SRAP molecular markers linked to salt tolerance in Zoysia grasses using two salt-tolerant, extreme types of DNA pools. From 400 pairs of SRAP primer combinations, 111 were found with polymorphic bands. These were further screened using two extreme salt-tolerant samples of Zoysia japonica and 22 pairs of polymorphism primer combinations were selected. Finally, the primer combinations with specific bands were verified using salt-tolerant individuals and 7 SRAP molecular markers closely linked to salt tolerance were obtained. Based on the size of the amplified bands they were named: Me9-Em4₂₆₀, Me9-Em18₇₂₀, Me13-Em18₅₀₀, Me5-Em20₁₈₀, Me14-Em7₂₂₀, Me6-Em17₅₅₀, Me2-Em18₂₆₀. This research will accelerate zoysia grass marker-assisted selection breeding and lay the foundation for salt-tolerant gene cloning.

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CHEN Xuan, GUO Hai-lin, XUE Dan-dan, ZHENG Yi-qi, LIU Jian-xiu. Identification of SRAP molecular markers linked to salt tolerance in Zoysia grasses[J]. Acta Prataculturae Sinica, 2009, 18(2): 66-75.

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Identification of SRAP molecular markers linked to salt tolerance in Zoysia grasses

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[1]	GUO Yu-pin,MI Fu-gui,YAN Li-jun,REN Yong-xia,LV Shi-jie,FU Bing-zhe. Physiological response to drought stresses and drought resistances evaluation of different Kentucky bluegrass varieties [J]. Acta Prataculturae Sinica, 2014, 23(4): 220-228.
[2]	YANG Hai-xia,LIU Run-jin,GUO Shao-xia. Effects of arbuscular mycorrhizal fungus Glomus mosseae on the growth characteristics of Festuca arundinacea under salt stress conditions [J]. Acta Prataculturae Sinica, 2014, 23(4): 195-203.
[3]	HAN Chao,LIU Yang,DONG Hui,CHANG Zhi-hui. Influences of biosolids on drought resistance of tall fescue [J]. Acta Prataculturae Sinica, 2014, 23(3): 127-135.
[4]	ZHANG Huai-shan, ZHAO Gui-qi, LI Meng-fei, XIA Zeng-run, WANG Chun-mei. Physiological responses of Pennisetum longissimum var. intermedium seedlings to PEG, low temperature and salt stress treatments [J]. Acta Prataculturae Sinica, 2014, 23(2): 180-188.
[5]	PENG Yan, LI Zhou. Effects of drought preconditioning on physiological responses to heat stress in two Kentucky bluegrasses [J]. Acta Prataculturae Sinica, 2013, 22(5): 229-238.
[6]	YU Le, LIU Yong-hai, ZHOU Li-ping, LIANG Guo-qiu. A study on the changes of ascorbic acid and related physiological indexes in different cultivars of Zoysia under drought stress [J]. Acta Prataculturae Sinica, 2013, 22(4): 106-115.
[7]	Drought tolerance analysis of Miscanthus sinensis ‘Gracillimu’ seedlingsCHEN Min, HOU Xin-cun, FAN Xi-feng, WU Ju-ying, PAN Yuan-zhi. Drought tolerance analysis of Miscanthus sinensis ‘Gracillimu’ seedlings [J]. Acta Prataculturae Sinica, 2013, 22(3): 184-.
[8]	JIANG Qiao-feng, CHEN Jing-bo, ZONG Jun-qin, LI Shan, CHU Xiao-qing, GUO Hai-lin, LIU Jian-xiu . Effect of phosphorus on Na⁺ and K⁺ concentrations and the growth of Zoysia matrella under salt stress [J]. Acta Prataculturae Sinica, 2013, 22(3): 162-.
[9]	LI Xiao-yu, LIN Ji-xiang, LI Xiu-jun, MU Chun-sheng. Growth adaptation and Na⁺ and K⁺ metabolism responses of Leymus chinensis seedlings under salt and alkali stresses [J]. Acta Prataculturae Sinica, 2013, 22(1): 201-209.
[10]	CHEN Jing-bo, LIU Jian-xiu. Salinity tolerance evaluation and mechanisms in bermudagrass (Cynodon spp.) [J]. Acta Prataculturae Sinica, 2012, 21(5): 302-310.
[11]	LI Shan, CHEN Jing-bo, GUO Hai-lin, ZONG Jun-qin, ZHANG Fang, CHU Xiao-qing, JIANG Qiao-feng, DING Wan-wen, LIU Jian-xiu. Salinity tolerance evaluation of Zoysia turfgrass germplasm [J]. Acta Prataculturae Sinica, 2012, 21(4): 43-51.
[12]	YUAN Xue-jun, WANG Zhi-yong, ZHENG Yi-qi, LIU Jian-xiu, SHE Jian-ming. Acquisition and identification of cold-resistant somatic mutants of centipedegrass [J]. Acta Prataculturae Sinica, 2011, 20(6): 237-244.
[13]	WANG Dan, XUAN Ji-ping, GUO Hai-lin, LIU Jian-xiu. Seasonal changes of freezing tolerance and its relationship to the contents of carbohydrates, proline, and soluble protein of Zoysia [J]. Acta Prataculturae Sinica, 2011, 20(4): 98-107.
[14]	DONG Qiu-li, XIA Fang-shan, DONG Kuan-hu. Effects of NaCl stress on proline metabolism of Achnatherum splendens seedling [J]. Acta Prataculturae Sinica, 2010, 19(5): 71-76.
[15]	WANG Yan, LI Jian-long, JIANG Tao, DENG Lei. Effect of pre-treatments with SA、H₂O₂ and 6-BA on chilling tolerance in Zoysia matrella [J]. Acta Prataculturae Sinica, 2010, 19(2): 76-81.