Transformation of Cichorium intybus with the AtNHX1 gene and salinity tolerance of the transformants

Abstract

Abstract: The AtNHX1 gene was transferred into Cichorium intybus using Agrobacterium and 42 regenerated plants resistant to Kanamycin (Kan) were obtained. Incorporation and expression of the AtNHX1 gene in the transformants were confirmed by PCR, Southern blot, and RT-PCR. Under the same salt stress conditions, the transgenic calli with NaCl resistance, that were induced from transgenic plants, had considerably higher relative growth rates than the wild-type calli. Under the stress of different concentrations of NaCl, the K⁺/Na⁺ ratio in the transgenic plant cells was always higher than that in the wild-type, whereas the relative conductivity was the opposite. The transformation of the AtNHX1 gene not only enhanced the salt tolerance of transgenic C. intybus, but also reduced cell membrane damage induced by salinity.

CLC Number:

ZHAO Yu-wei, WANG Ying-juan, BU Huai-yu, HAO Jian-guo, JIA Jing-fen. Transformation of Cichorium intybus with the AtNHX1
gene and salinity tolerance of the transformants[J]. Acta Prataculturae Sinica, 2009, 18(3): 103-109.

References

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Transformation of Cichorium intybus with the AtNHX1
gene and salinity tolerance of the transformants

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