Abstract: A vacuolar-typed Na⁺/H⁺ antiporter gene was isolated from the halophyte L. gmelinii by RT-PCR and RACE. The LgNHX1 cDNA was 2 397 bp including a 5′untranslated region of 512 bp and a 3′-nontranslated region of 229 bp with poly A, and an open reading frame of 1 656 bp, encoding a predicted polypeptide of 550 amino acids with a predicted molecular mass of 61 kD. It was shown by multiple sequence alignment analysis that the LgNHX1 were 82.62%, 82.01%, 80.64% and 75.86% identitical in amino acids to the vacuolar-typed Na⁺/H⁺ antiporter genes from Atriplex gmelini, Tetragonia tetragonioides, Salicornia europaea and Arabidopsis thaliana, respectively. TMpred prediction showed 11-12 transmembrane domains of LgNHX1. Phylogenetic analysis indicates that LgNHX1 is more related to the vacuolar-typed Na⁺/H⁺ antiporter, compared with the plasma membrane-type Na⁺/H⁺ antiporter, to which it is less related.