A study on the diversity of soil cultured fungi in the alpine grassland of Eastern Qilian Mountains

Abstract

Abstract: In order to assess the diversity of soil cultured fungi in alpine grassland of Estern Qilian Mountains, 4 types alpine grasslands (Polygonum grassland, grass grassland, swamp grassland, Kobresia grassland) were selected to investigate their soil cultured fungi to understand their diversity by dilution plate technique and ITS rDNA sequence analysis, the soil cultured fungi were evaluted by economic efficiency analysis methods. 76 strains fungi were isolated by PDA, PSA, Maize Powder Media and Rose Bengal Medium. 30 typical strains were analyzed by ITS rDNA sequence analysis.The resulted showed, 30 strains belong to 22 genera, 26 species, most of them belong to imperfect fungi and zygomycetes. Species abundance (S), Shannon-Wiener index (H), Simpson dominance index (D) and Pielou evenness index (J) range were 15-18, 2.47-2.81, 0.89-0.93, 0.91-0.97, respectively. Penicillium spp. were dominant fungi in Polygonum grassland, swamp grassland and Kobresia grassland, Fusarium spp. were dominant fungi in grass grassland, Doratomyces spp. were dominant fungi in swamp grassland, Mortierella, Doratomyces, Leptosphaeria, Mucor, Trichoderma, Geomyces and Fusarium were common genera in four types grassland. In addition, more than 10% fungi strains could not clarify the taxonomic status temporarily, which might be new species; The results indicate that the diversity of soil fungi were very abundant in the alpine grassland of Eastern Qilian Mountains, and there exist close corelation between fungal diversity and grassland types, finally, it is concluded that there have abundance soil fungi in the alpine grassland of Eastern Qilian Mountains, which were deserved for further study and having huge exploitation value.

CLC Number:

ZHANG Jun-zhong, CHEN Xiu-rong, YANG Cheng-de, XUE Li. A study on the diversity of soil cultured fungi in the alpine grassland of Eastern Qilian Mountains[J]. Acta Prataculturae Sinica, 2010, 19(2): 124-132.

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