Abstract: Based on the evaluation of aluminum tolerance among 48 centipedegrass accessions, the difference of growth index and reactive oxygen system of Al-tolerant and Al-sensitive centipedegrass accessions after growing in aluminum soil for 28 days was assessed. Significant differences were found among five growth indexes, but not plant height. There was a considerable negative effect of aluminum stress on root length, number of roots, root volume, numbers of tillers and stolon length of 10 centipedegrass, with an average reduction of 16.4%, 23.9%, 37.3%, 26.7%, 18.3% respectively for these growth indexes in Al-tolerant accessions and 34.4%, 53.8%, 51.5 %, 52.6%, 47.9% in Al-sensitive accessions. The dynamic changes of growth in the 10 accessions indicated that tolerant accessions could maintain a higher growth rate compared with sensitive accessions when growing in the aluminum soil. The rate of superoxide anion radical formation and the MDA content of centipedegrass increased under aluminum stress, and the increased range of sensitive accessions was higher than that of the tolerant accessions. The aluminum stress resulted in an increase of SOD and POD activity, which was greater in the tolerant accessions than that in the sensitive accessions, suggesting that centipedegrass could alleviate the effects of aluminum toxicity by increasing SOD and POD activity. The production of SOD and POD among the Al-tolerant accessions was quicker than that of the sensitive accessions when suffering aluminum stress.