Abstract: Following leaf application of signaling compounds such as SA, H₂O₂, and 6-BA, the effect of three pretreatments on the chilling tolerance in manila grass was studied. SA, H₂O₂, and 6-BA pretreatments improved the chilling tolerance of manila grass by controlling and preventing the accumulation of reactive oxygen species and the occurrence of oxidative damage. POD, CAT, and APX activities in pretreatments were significantly higher than in controls, and SOD activities were increased by chilling itself. GR may regulate the oxidant-reduction status as feedback, indicating that these antioxidative enzymes were closely related to the improvement of cold resistance in manila grass. SA induced more SOD, CAT, and GR activities than other compounds, suggesting SA may be the most effective of the four compounds for improving the chilling tolerance of manila grass.