Constructing expression vector of genes phaB and phaC drived by specific promoter LTP12 for cotton fiber

Abstract

Abstract: The poly-3-hydroxybutyrate(PHB) possesses anti-wrinkle and stay-warm characteristics. It has been hypothesized that the anti-wrinkle and stay-warm ability of cotton fiber would be improved if fiber contains PHB. The genetic engineered cotton plant containing the PHB synthesis would theoretically increase the incorporation of PHB into cotton fiber. As a result, the waterproof ability, anti-wrinkle and stay-warm characteristics of cotton fiber should be improved. In this study, the cotton fiber specific promoter LTP12 and two PHB synthesis genes (phaB and phaC) were integrated into a pBI121. Therefore, a new plant expression vector pBLTPPBPC was constructed.

CLC Number:

Q943.2

MENG Ya-xiong, ZHANG Jin-wen, MA Xiao-le, ZHANG Jin-lin, ZHONG Jun, WANG Hua-jun. Constructing expression vector of genes phaB and phaC drived by specific promoter LTP12 for cotton fiber[J]. Acta Prataculturae Sinica, 2010, 19(3): 170-176.

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