Analysis of cloning and sequence characteristics of cDNA encoding the GBSS I gene from tubers of Solanum tuberosum

Abstract

Abstract: The cDNA clone encoding GBSSⅠwas obtained by extracting total RNA from tubers of Solanum tuberosum CV. Gannongshu NO.2 using an RNA isolation system, RT-PCR (reverse transcription-polymerase chain reaction), then measuring and analyzing the DNA sequence. The full-length of cDNA was 1 824 bp, which contained 607 amino acids and a termination codon: the homology was 99.78% compared with the original sequence (Accession Number X58453), but the homologies were very low compared with other families. The gene has the registration number EU403426 in GenBank. The protein function and structure of the GBSSⅠgene cDNA sequence were predicted and analyzed using related software of bioinformatics. The protein had three completely conserved domains as in other GBSS proteins of 15 plants and it had a number of important functional sites. It also had a tertiary structure similar to starch synthase of Agrobacterium, which suggests that the protein functioned in starch synthesis.

CLC Number:

SHEN Bao-yun, LIU Yu-hui, ZHANG Jun-lian, WANG Di. Analysis of cloning and sequence characteristics of cDNA encoding the GBSS I gene from tubers of Solanum tuberosum[J]. Acta Prataculturae Sinica, 2010, 19(5): 1-8.

References

[1] Visser R G F, Jacobsen E. Towards modifying plants for altered starch content and composition[J]. Trends Biotechnol, 1993, 11: 63-68.
[2] Li Z, Rahman S, Kosar-Hashemi B, et al. Cloning and characterization of a gene encoding wheat starch synthase I[J]. Theoretical and Applied Genetics, 1999, 98: 1208-1216.
[3] 于天峰. 马铃薯淀粉的糊化特性、用途及品质改良[J]. 中国马铃薯, 2005, 19(4): 223-225.
[4] 秦波涛, 李和平, 王晓曦. 薯类加工[M]. 北京:中国轻工业出版社, 2001.
[5] 王中荣, 刘雄. 高直链淀粉性质及应用研究[J]. 粮食与油脂, 2005, 11: 10-13.
[6] 王立梅, 齐斌. 直链淀粉含量的影响因素及其应用研究进展[J]. 食品科学, 2007, 28(10): 604-608.
[7] 于天峰, 夏平. 马铃薯淀粉特性及其利用研究[J]. 中国农学通报, 2005, 21(1): 55-58.
[8] 陈芳, 赵景文, 胡小松. 我国马铃薯加工业的现状、问题及发展对策[J].中国农业科技导报, 2002, 4(2): 66-68.
[9] Sambrook J, RuSSell D W. 分子克隆实验指南(第三版)[M]. 黄培堂, 译. 北京: 科学技术出版社, 2002.
[10] Feike R, vander Leij F R, Richard G F, et al. Sequence of the structural gene for granule_bound starch synthase of potato (Solanum tuberosum L.) and evidence for a single point deletion in the amf allele[J]. Molecular and General Genetics, 1991, 228: 240-248.
[11] 彭佶松, 吴晓俊, 刘涤, 等. 黄芪毛状根GBSSI基因cDNA克隆及其结构分析[J]. 植物学报, 2000, 42(9): 940-945.
[12] Dry I, Smith A, Edwards A, et al. Characterization of cDNAs encoding two isoforms of granule-bound starch synthase which show differential expression in developing storage organs of pea and potato[J]. Plant Journal, 1992, 2(2): 193-202.
[13] Fulton D C, Edwards A, Pilling E. Role of granule-bound starch synthase in determination of amylopectin structure and starch granule morpholpgy in potato[J]. Biochemical Journal, 2002, 277(13): 10834-10841.
[14] 戴朝曦, 孙顺娣, 于品华, 等. 用生物技术培育马铃薯加工型品种的研究[A]. 陈伊里: 面向21世纪的中国马铃薯产业[M]. 哈尔滨: 哈尔滨工程大学出版社, 2000: 103-107.
[15] 李加瑞, 赵伟, 李全梓, 等. Waxy基因的RNA沉默使转基因小麦种子中直链淀粉含量下降[J]. 遗传学报, 2005, 32(8): 846-854.
[16] 史振声, 王志斌, 李凤海, 等. 国内外高直链淀粉玉米的研究与利用[J]. 辽宁农业科学, 2002, 1(6): 30-33.
[17] 黄晓杰, 张春红, 赵前程, 等. 高直链玉米淀粉-PVA共混塑料薄膜的制作工艺的研究[J]. 食品工业科技, 2006, 3:160-161.
[18] 谷宏, 马涛, 赵增煜. 高直链玉米淀粉可食性膜的研制[J]. 包装工程, 2007, 28(5): 15-17.
[19] Christine H H, Walter M, Hermann H. Detection of the starch modifying gbss-antisense construct in transgenic potatoes[J]. Zeitschrift für Lebensmittel-Untersuchung und-Forschung A, 1998, 206: 83-87.
[20] 张改娜, 贾敬芬. 豌豆清蛋白1(PA1)基因的克隆及对苜蓿的转化[J]. 草业学报, 2009, 18(3): 117-125.
[21] 吴建明, 李杨瑞, 王爱勤, 等. 甘蔗GA20氧化酶基因片段克隆及序列分析[J]. 热带作物学报, 2009, 30(6): 817-821.
[22] 尚以顺, 杨春燕, 钟理. 贵州野生匍匐剪股颖种质资源遗传多样性的ISSR分析[J]. 草业学报, 2009, 18(3): 67-73.
[23] 吴旺泽, 彭晓莉, 王晓明, 等. 马铃薯水通道蛋白基因cDNA克隆、序列分析及表达[J]. 农业生物技术学报, 2007, 15(4): 677-683.
[24] 郑轶琦, 刘建秀. 草坪草分子遗传图谱的构建与应用研究进展[J]. 草业学报, 2009, 18(1): 155-162.