Establishment of a high frequency regeneration system for the succulent xerophyte Zygophyllum xanthoxylum

Abstract

Abstract: The effect of compounding proportions of different hormones on explants (cotyledon, stem, eustipes, and hypocotyl) were studied in tissue cultures of the succulent xerophyte Zygophyllum xanthoxylum, and a stable, high-efficiency plant regeneration system was established. Cotyledons were the best type of explant for callus induction and eustipes were the best for adventitious bud induction. The best callus inducing medium for Z. xanthoxylum was MS containing 0.1 mg/L 6-BA and 1.0 mg/L NAA, in which the callus induction rate for cotyledons was 100% with an induction time of 20 d, shorter than the previously reported 30 d. The best medium to induce adventitious buds was MS containing 1.5 mg/L 6-BA and 0.5 mg/L NAA, in which shoot differentiation of eustipes reached 82.5% and the induction time wais 7-weeks, shorter than the earlier report of 10-weeks. The best medium for rooting was 1/2 MS containing 1.0 mg/L NAA and 0.5 mg/L IBA, in which the frequency of rooting reached 86.7%, 13.4% greater than the previous result of 73.3%. In this medium, adventitious roots began to appear in 13 d (earlier than the reported 20 d) and the transition from callus to regeneration plant was completed in (84±4) d.

CLC Number:

Q945.3

FENG Bo, DUAN Jiao-jiao, WU Guo-qiang, WANG Suo-min. Establishment of a high frequency regeneration system for the succulent xerophyte Zygophyllum xanthoxylum[J]. Acta Prataculturae Sinica, 2010, 19(6): 140-146.

References

[1] 中国科学院中国植物志编辑委员会. 中国植物志(第43卷,第一分册)[M]. 北京: 科学出版社, 1998: 140-142.
[2] 周向睿, 周志宇, 吴彩霞. 霸王繁殖特性的研究[J]. 草业科学, 2006, 23(6): 38-41.
[3] Wang S M, Wan C G, Wang Y R, et al. The characteristics of Na⁺, K⁺ and free proline distribution in several drought-resistant plants of the Alxa Desert, China[J]. Journal of Arid Environments, 2004, 56: 525-539.
[4] 张志勇, 胡相伟. 霸王的组织培养和植株再生[J]. 植物生理学通讯, 2007, 43(3): 495.
[5] 孙兰弟, 管利萍, 张颖聪, 等. 沙生濒危物种霸王的高频植株再生研究[J]. 中国沙漠, 2009, 29(2): 312-315.
[6] Sun L D, Hou S W, Wu D, et al. Rapid clonal propagation of Zygophyllum xanthoxylon(Bunge) Maxin., an endangered desert forage species[J]. In Vitro Cell Development of Biology-Plant, 2008, (44): 369-400.
[7] 王瑛华, 陈刚, 贾敬芬, 等. 霸王的原生质体培养及植株再生研究[J]. 草业学报, 2009, 18(3): 110-116.
[8] 颜吕敬. 植物组织培养手册[M]. 上海: 上海科学技术出版社, 1990.
[9] 梁哲, 姜三杰, 未丽, 等. 三叶草基因工程研究进展[J]. 草业学报, 2009, 18(2): 205-211.
[10] 李浚明. 植物组织培养教程(第2版)[M]. 北京: 中国农业大学出版社, 2002.
[11] 黄卓烈, 李明. 吲哚丁酸对桉树插条多酚氧化酶的影响及其与生根的关系[J]. 广西植物, 2003, 23(1): 77-82.
[12] 朱晓花, 孙吉雄, 梁慧敏, 等. 低温预处理与植物生长调节剂对结缕草愈伤组织诱导的影响[J]. 草业科学, 2009, 26(4): 121-126.
[13] 李代丽, 康向阳. 植物愈伤组织培养中内外源激素效应的研究现状与展望[J]. 生物技术通讯, 2007, 18(3): 546-548.
[14] 徐春波, 米福贵, 王勇, 等. 影响冰草成熟胚组织培养再生体系频率的因素[J]. 草业学报, 2009, 18(1): 80-85.
[15] 张洋, 陈志, 王慧春. 2,4-D和6-BA对唐古特大黄愈伤组织诱导的影响[J]. 河南科技学院学报(自然科学版), 2006, 34(2): 33-35.
[16] 杜雪玲, 张振霞. 2,4-D和6-BA对多年生黑麦草愈伤组织诱导影响的研究[J]. 草原与草坪, 2005, (3): 49-51.
[17] 贾秀萍, 王汉宁, 张金文, 等. 2,4-D和NAA对成熟玉米种子不同外植体胚性愈伤组织诱导效果的影响[J]. 甘肃农业大学学报, 2008, 43(6): 48-51.
[18] 赵一之, 宋宗元. 亚洲中部荒漠区的植物特有属[J]. 云南植物研究, 2003, 25(2): 113-121.
[19] 裘文达. 园艺植物组织培养[J]. 上海: 上海科学出版社, 1986.
[20] 谭文澄, 戴策刚. 观赏植物组织培养技术[M]. 北京: 中国林业出版社, 1991.
[21] Zenk M H, Shasi E L, Arens H, et al. Formation cultures of Catharanthus[J]. Plant Tissue Culture and Its Biotechnological Application, 1977, 3: 27-43.
[22] 韦弗. 农业中的植物生长物质[M]. 北京: 科学出版社, 1979.