SSR analysis of the linkage drag of endosperm mutation gene ae

Abstract

Abstract: Molecular assisted selection was used to analyse linkage drag of different recurrent parents on two flanks of two populations and for restoring the ratio of two populations by SSR markers for ae. These are evenly distributed on chromosome 5. Linkage drags at the two flanks were controlled in 13.2 and 17.2 cM of CHBC₁F₂ populations by SSR markers and the linkage drag at one flank was controlled in 13.9 cM of DHBC₁F₂ populations. Genetic background resemblance of individuals of the CHBC₁F₂ population to the recurrent parent’s was 85.7%. Genetic background resemblance of individuals of the DHBC₁F₂ population to the recurrent parent’s was 87.5%. It is possible to identify recombination individuals with the targeted gene through a high density (10-20 cM per marker) map. This reduced unnecessary fragmentation of chromosomes and eliminated linkage drag of the targeted gene in backcross breeding. The hitchhiking effect may have been reduced.

CLC Number:

QIAO Yan, WANG Han-ning, ZHANG Cheng, YANG Fang. SSR analysis of the linkage drag of endosperm mutation gene ae[J]. Acta Prataculturae Sinica, 2011, 20(1): 140-147.

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