Abstract: Insecticidal activity of extracts from leaves of Cichorium intybus, were assessed by cold-soaked extraction and by microwave-assisted extraction. The insecticidal activities of the C. intybus leaf extracts against the larvae of Mythimna separata were tested by a feeding, weighing and leaf disk method. The body weights of larvae fed by the extracts with organic solvents were significantly lower than those of the controls in the first seven days, and the development period of larvae treated with ethanol or ethyl acetate extracts were 4.3 days longer than that of the controls. The corrected mortality of M. separata treated by the ethyl acetate extracts was the highest (57.78%), and the corresponding pupating rate was the lowest (46.67%). Moreover, the pupal weight in the different treatments was greater than that of the controls. In anti-feeding experiments, the anti-feeding rates of ethanol extracts and ethyl acetate extracts against M. separata were more than 80%.