Cloning and expression analysis of aldehyde dehydrogenase gene LcALDH from Leymus chinensis

Abstract

Abstract: In this research, cDNA(GenBank No. EF492045) of aldehyde dehydrogenase gene LcALDH was isolated from Leymus chinensis by RACE technology.The nucleotide sequence of LcALDH cDNA is 1 712 bp containing an open reading frame of 1 503 bp encoding 500 amino acids, with a 5′untranslated region of 66 bp and a 3′untranslated region of 144 bp. The amino acid sequence contains absolutely conservative glutamic acid and cysteine residues active sites of aldehyde dehydrogenase family. The analysis of LcALDH gene expression under different stress condition showed that LcALDH expression was positively regulated by low-temperature, drought, high salt and ABA. The research results will lay the foundation for both the further exploration of the stress resistant mechanism of L. chinensis from molecular level, and the utilization of plant gene.

CLC Number:

LI Xin-ling, WU Shu-ju, WANG Quan-wei. Cloning and expression analysis of aldehyde dehydrogenase gene LcALDH from Leymus chinensis[J]. Acta Prataculturae Sinica, 2011, 20(4): 187-193.

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