Seasonal changes of freezing tolerance and its relationship to the contents of carbohydrates, proline, and soluble protein of Zoysia

Abstract

Abstract: To clarify the changes of carbohydrates, proline, soluble protein contents and their relationships to freezing tolerance of Zoysia japonica ‘Lanyin No.3’, the relationship of carbohydrate (total soluble sugars, sucrose, glucose, starch), proline and soluble protein concentrations with LT₅₀(the lethal temperature killing 50% of the plants) of rhizomes were studied at monthy intervals from October, 2008 to May, 2009 under natural cold conditions. The contents of carbohydrates, proline, soluble protein and LT₅₀ showed a clear seasonal trend in rhizomes of Z. japonica ‘Lanyin No.3’ and they increased or decreased sharply as temperatures dropped in overwintering and decreased or increased gradually along with the rise in temperature in spring. Correlation analysis revealed that the freezing tolerance of rhizomes of Z. japonica ‘Lanyin No.3’ positively correlated with the contents of total soluble sugars, glucose and the ratio of the soluble sugars to starch but had no positive correlation with the contents of sucrose, starch, and proline.

CLC Number:

WANG Dan, XUAN Ji-ping, GUO Hai-lin, LIU Jian-xiu. Seasonal changes of freezing tolerance and its relationship to the contents of carbohydrates, proline, and soluble protein of Zoysia[J]. Acta Prataculturae Sinica, 2011, 20(4): 98-107.

References

[1] 王艳. 结缕草研究进展[J]. 中国草地, 2003, 25(2): 45-53.
[2] 刘建秀, 贺善安. 华东地区结缕草属植物形态类型及坪用价值[J]. 草地学报, 1997, (1): 42-47.
[3] 王艳, 李建龙, 邓蕾, 等. 4种结缕草在南京地区的田间表现及耐寒性差异[J]. 中国草地学报, 2009, 31(6): 34-38.
[4] 郭海林, 高雅丹, 薛丹丹, 等. 结缕草属植物抗寒性的遗传分析[J]. 草业学报, 2009, 18(3): 53-58.
[5] 王艳, 李建龙, 姜涛, 等. SA、H₂O₂和6-BA预处理对沟叶结缕草耐寒性的影响[J]. 草业学报, 2010, 19(2): 76-81.
[6] Levitt J. Responses of Plants to Environmental Stress[M]. New York: Academic Press, 1980.
[7] 潘瑞炽, 董愚得. 植物生理学[M]. 北京: 高等教育出版社, 1995.
[8] 王孝宣, 李树德, 东惠茹, 等. 番茄品种耐寒性与可溶性糖含量的关系[J]. 园艺学报, 1998, 25(1): 56-60.
[9] Akiyama F, Sasaki H, Saito Y. Seasonal changes of contents of soluble amino acids and oligosaccharides in turfgrasses (Zoysia)[J]. National Grassland Research Institute, 1994, 49: 25-33.
[10] Fry J D, Lang N S, Clifton R G P, et al. Freezing tolerance and carbohydrate content of low-temperature acclimated and nonacclimated centipedegrass[J]. Crop Science, 1993, 33: 1051-1055.
[11] Ball S, Qian Y L, Stushnoff C. Soluble carbohydrates in two buffalograss cultivars with contrasting freeze tolerance[J]. Journal of the American Society for Horticultural Science, 2002, 123: 45-49.
[12] Dionne J, Castonguay Y, Nadeau P, et al. Freezing tolerance and carbohydrate changes during cold acclimation of green-type annual bluegrass(Poa annal L.)ecotypes[J]. Crop Science, 2001, 41: 443-451.
[13] Maier F P, Lang N S, Fry J D. Freezing tolerance of three St. Augustinegrass cultivars as affected by stolon carbohydrate and water content[J]. Journal of the American Society for Horticultural Science, 1994, 119: 473-476.
[14] Dunn J H, Nelson C J. Chemical changes occurring in three bermudagrass turf cultivars in relation to cold hardiness[J]. Agronomy Journal, 1974, 66: 28-31.
[15] Patton A J, Cunningham S M, Volenec J J, et al. Differences in freeze tolerance of zoysiagrasses: II. Carbohydrate and proline accumulation[J]. Crop Science, 2007, 47: 2170-2181.
[16] Bush E, Wilson P, Shepard D, et al. Freezing tolerance and nonstructural carbohydrate composition of carpetgrass (Axonopus affinis Chase)[J]. HortScience, 2000, 35: 187-189.
[17] Rogers R A, Dunn J H, Nelson C J. Photosynthesis and cold hardening in zoysia and bermudagrass[J]. Crop Science, 1977, 17: 723-732.
[18] 杜永吉, 于磊, 孙吉雄, 等. 结缕草3个品种抗寒性的综合评价[J]. 草业学报, 2008, 17(3): 6-16.
[19] 刘志雄, 周高峰, 廖启荣, 等. 低温胁迫对几个狗牙根品种生理生化特征的影响[J]. 安徽农学通讯, 2007, 13(4): 23-25.
[20] 袁学军, 刘建秀, 张婷婷, 等. 硝酸钾对假俭草抗寒性和草绿期的影响[J]. 草业学报, 2007, 16(4): 363-370.
[21] Cai Q, Wang S, Cui Z, et al. Changes in freezing tolerance and its relationship with the contents of carbohydrates and proline in overwintering centipedegrass(Eremochloa ophiurpides (Munro) Hack.)[J]. Plant Production Science, 2004, 7(4): 421-426.
[22] 池春玉, 丁国华, 连永权, 等. 低温胁迫对三种冷季型草坪草脯氨酸含量及膜透性的影响[J]. 中国农学通报, 2007, 23(1): 101-103.
[23] Patton A J, Cunningham S M, Volenec J J, et al. Differences in freeze tolerance of zoysiagrasses: I. Role of protein[J]. Crop Science, 2007, 47: 2162-2169.
[24] Gatschet M J, Taliaferro C M, Andenson J A, et al. Cold acclimation and alterations in protein synthesis in bermudagrass crowns[J]. HortScience, 1994, 119(3): 477-480.
[25] Gatschet M J, Taliaferro C M, Andenson J A, et al. A cold-regulated protein from bermudngrass crowns is a chitinase[J]. Crop Science, 1996, 36: 712-718.
[26] Palmertree H D. The influence of mineral nutrition on the tolerance, nutrient content, and soluble protein fraction centipedegrass(Eremochloa ophiuroides (Munro) Hack.)[J]. Dissertation Abstracts International, 1973, 33(7): 2894-2895.
[27] 赵鸣. 兰引3号结缕草坪用性状及适应性研究[J]. 草原与草坪, 2000, (3): 34-36.
[28] 朱根海, 朱培仁. 小麦抗冻性的季节变化及温度对脱锻炼的效应[J].南京农学院学报, 1984, 7(2): 9-16.
[29] 中国科学院上海植物生理研究所, 上海市植物生理学会. 现代植物生理学实验指南[M]. 北京: 科学出版社, 2004: 127-128.
[30] 郭冬生, 彭小兰. 蒽酮比色法和酶水解法两种淀粉测定方法的比较研究[J]. 湖南文理学院学报, 2007, 19(3): 34-36.
[31] 张立军, 樊金娟. 植物生理学实验教程[M]. 北京: 中国农业大学出版社, 2007: 94-96.
[32] 李合生. 现代植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000: 184-185.
[33] 莫惠栋. Logistic方程及其应用[J]. 江苏农学院学报, 1983, 4(2): 53-57.
[34] 王丹, 宣继萍, 郭海林, 等. 暖季型草坪草不同营养器官耐寒力的动态变化[J]. 草业科学, 2010, 27(3): 26-30.
[35] Beard J B. Direct low temperature injury of nineteen turfgrass[J]. Quart. Bull. Michigan State University Agricultural Experiment Station, 1966, 48(3): 377-383.
[36] Rogers R A, Dunn J H, Nelson C J. Cold hardening and carbohydrate composition of Meyer zoysia[J]. Agronomy Journal, 1975, 67: 836-838.
[37] Shahba M A, Qian Y L, Hughes H G, et al. Cold hardiness of saltgrass accessions[J]. Crop Science, 2003, 43: 2148-2153.
[38] 魏臻武, 范占炼, 王槐三. 不同类型草坪草的抗寒锻炼[J]. 草业科学, 1997, (3): 60-65.
[39] White R H, Schmidt R E. Fall performance and post-dormancy growth of ‘Midiron’ bermudagrass in response to nitrogen, iron and benzyladenine[J]. Journal of the American Society for Horticultural Science, 1990, 115: 57-61.
[40] Shahba M A, Qian Y L, Hughes H G, et al. Relationships of soluble carbohydrates and freeze tolerance in saltgrass[J]. Crop Science, 2003, 43: 2148-2153.
[41] Fry J D, Lang N S, Clifton R G P. Freezing resistance and carbohydrate composition of ‘Flortam’ St. Augustinegrass[J]. HortScience, 1991, 26: 1537-1539.
[43] 李合生. 现代植物生理学[M]. 北京: 高等教育出版社, 2004: 4.
[44] Munshaw G C, Ervin E H, Shang C, et al. Influence of late-season iron, nitrogen, and seaweed extract on fall color retention and cold tolerance of four bermudagrass cultivars[J]. Crop Science, 2006, 46: 273-283.
[45] Paquin R. Effet des basses températures sur la résistance au gel de la Luzerne (Medicago media Pers.) et son contenuen proline libre[J]. Physiology Vegetable, 1977, 15: 657-665.
[46] Doffling K, Doffling H, Lesselich G, et al. Heritable improvement of frost tolerance in winter wheat by in vitro-selection of hydroxyl proline-resistant proline over production mutants[J]. Euphytica, 1997, 93: 1-10.
[47] 黄锦文, 陈冬梅, 郑红艳, 等. 暖季型结缕草对低温响应的生理生态特性[J]. 中国草地学报, 2009, 31(1): 64-69.
[48] Davis D L, Gilbert W B. Winter hardiness and changes in soluble protein fraction of bermdagrass[J]. Crop Science, 1970, 10: 7-9.
[49] Trunova J I. Winter wheat frost hardiness and protein synthesis at chilling temperature[A]. In: Li P H. Plant Cold Hardiness[M]. New York: Alan R. Liss, 1987: 43-47.
[50] 柴团耀, 张玉秀. 菜豆富含脯氨酸、蛋白质基因在生物和非生物胁迫下的表达[J]. 植物学报, 1999, 41(1): 111-113.