Establishment of an Agrobacterium tumefaciens-mediated genetic transformation system of Lilium speciosum

Abstract

Abstract: Several factors, affecting Agrobacterium tumefaciens-mediated genetic transformations of lily were studied based on the establishment of regeneration systems. The pre-culture time of 5 days was beneficial for improving transformation frequency. Co-culture for 3 days with 100 μmol/L AS (acetosyringone) resulted not only in higher rates of transformation but also controlled the over-growth of bacteria. The 10-min bacteria infection (ABS₆₀₀= 0.8, pH=5.7) with 150 μmol/L AS was found to be the optimal selection for transgenic plantlets. It was confirmed by using gus instantaneous expression test and hpt gene PCR test that the vector was integrated into the regeneration genome of lily.

CLC Number:

CHU Jun, XU Na, ZHANG Qiang, WANG Song-hua, ZHOU Zheng-yi, JIN Guang-ming. Establishment of an Agrobacterium tumefaciens-mediated genetic transformation system of Lilium speciosum[J]. Acta Prataculturae Sinica, 2011, 20(6): 164-169.

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