Identification of cellulose decomposing fungi strain F1 and decomposition activity to two kinds of lawn grass litter

Abstract

Abstract: A fungal isolate (strain F₁) was isolated from east part of grassland soil in Qilian alpine. Taxonomic status and decomposition activity to two kinds of lawn grass litter were studied in this study. Combinated morphology with rDNA-ITS molecular biology methods, the fungus was Microdochium bolleyi. The dynamics of lignocellulolytic enzymes secreted by M. bolleyi and cell wall material(CWM) decomposition of perennial ryegrass (Lolium perenne) and white clover (Trifolium repens) litter were investigated for 42 d at 25℃ on a rotary shaker at 150 r/min in flask liquid fermentation method. 1) Insoluble cell wall material(ICWM) of two kinds of lawn grass litter significantly reduced after inoculation strain F₁ (P<0.05); 2) When two kinds of lawn grass litter acts as substrate respectively, the laccase secreted by strain F₁ appears first, followed by the cellulase enzyme and xylanase, catechol oxidase and guaiacol oxidase occurs at the latest; 3) Perennial ryegrass compared with white clover, the insoluble cell wall material (ICWM) and cell wall material (CWM) degradation rate of perennial ryegrass litter was higher (P<0.05); 4) The FPAase, CMCase, xylanase, pycocatechol oxidase and guaiacol oxidase activity except for the laccase secreted by M. bolleyi in perennial ryegrass litter were higher than those in white clover litter (P<0.05).

CLC Number:

LU Guang-xin, CHEN Xiu-rong, YANG Cheng-de, XUE Li, LIU Wen, BIAN Jing. Identification of cellulose decomposing fungi strain F₁ and decomposition activity to two kinds of lawn grass litter[J]. Acta Prataculturae Sinica, 2011, 20(6): 170-179.

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Identification of cellulose decomposing fungi strain F₁ and decomposition activity to two kinds of lawn grass litter

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