Abstract: Salt stress affects plant growth and development and reduces productivity of crops. This experiment was carried out to determine the MDA, plasma membrane ATP enzyme (PM-ATPase) and 5′-neucleotidase (5′-AMPase) of Saussurea runcinata seedling by NaCl and Na₂CO₃ stress (The concentration of Na⁺ is 0, 60, 120, 180, 240, 300 and 360 mmol/L) in order to assess the effects of its membrane systemthe under salinity and salt tolereance about it. It has been shown that the MDA increased by salinity stress. The content of MDA in roots and leaves of S. runcinata reached the maximum under Na₂CO₃ stress in the Na⁺ concentration of 360 mmol/L, respectively, 2.15 times and 1.94 times than the control. The maximum content of MDA in leaves of S. runcinata 1.22 times than the control. The activity of PM-ATPase and 5′-AMPase in the roots is higher than those in the leaves under the same concentration of Na⁺. The activity of PM-ATPase and 5′-AMPase were significantly difference by two different salinity stress. The maximum activity of PM-ATPase in the roots of S. runcinata up to 56.29 μg pi/(mg protein·h),but the activity of 5′-AMPase up to 53.91 μg pi/(mg protein·h). In a word, the damage of the roots of the S. runcinata is smaller than the leaves under the salinity stress, the S. runcinata more resistant to NaCl stress.