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Acta Prataculturae Sinica ›› 2012, Vol. 21 ›› Issue (5): 128-133.

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Comparison of SRAP and SSR markers for hybrid identification of switchgrass (Panicum virgatum)

ZHANG Jing1, HUANG Lin-kai1, ZHAO Bing-yu2, ZHANG Xin-quan1, YAN Hai-dong1, JIANG Xiao-yang1   

  1. 1.Department of Grassland Science, Sichuan Agricultural University, Ya’an 625014, China;
    2.Virginia Polytechnic Institute and State University, Blacksburg VA24061, America
  • Received:2011-10-24 Online:2012-05-25 Published:2012-10-20

Abstract: To provide a scientific basis for hybrid identification of switchgrass (Panicum virgatum) and cross-pollinating plants, we compared the identification efficiency and accuracy of SRAP and SSR markers. Switchgrass hybrid individuals (165) and their parental lines of switchgrass were tested by both methods. Compared to SRAP markers, the codominant SSR technique was more efficient. One pair of SSR primers (primer 23) detected all hybrids effectively and the percentage of polymorphic bands was 100%. SRAP required six primers to identify hybrids. The percentage of polymorphic bands of SRAP was 40% lower than that of SSR.

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