Transformation of the GsCRCK gene into Medicago sativa cv. Nongjing No.1 and salt tolerance analysis in transgenic plants

Abstract

Abstract: The stress-responsive kinase gene of wild soybean (GsCRCK) was selected because of the gene expression profiles under salinity, drought and cold stresses, previously established in our laboratory. Over expression of GsCRCK in transgenic Arabidopsis resulted in enhanced plant tolerance to high salinity and ABA. In this study, one plant expression vector regulated by a 35S promoter (named as pBEOCRCK) were constructed to transfer the target gene into Medicago sativa cv. Nongjing No.1 separately by the Agrobacterium-mediated method. Many resistant seedlings of GsCRCK have been obtained. The results of PCR and RT-PCR showed that the GsCRCK gene was normally expressed in transgenic plants. After GsCRCK transgenic M. sativa cv. Nongjing No.1 were stressed with 0, 300 and 400 mmol/L NaCl, we compared transgenic M. sativa to non-transgenic M. sativa by assessing specific physiological indicators. The relative membrane permeability, the content of chlorophyll, the content of MDA, and the activity of SOD in plants which were stressed with 300 mmol/L NaCl were measured together with the death rate from a 400 mmol/L NaCl stress. The transgenic M. sativa had a greater tolerance of salinity stress compared with non-transgenic plants.

CLC Number:

LIU Ying, CAI Hua, LIU Jing, BAI Xi, JI Wei, ZHU Yan-ming. Transformation of the GsCRCK gene into Medicago sativa cv. Nongjing No.1 and salt tolerance analysis in transgenic plants[J]. Acta Prataculturae Sinica, 2013, 22(2): 150-157.

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