Isolation, identification and analysis of rDNA-ITS gene sequence of Fusarium culmorum from maize ear rot samples

Abstract

Abstract: To identify pathogens of maize ear rot in Gansu province, samples were collected from 4 ecological zones in September, 2009 and the pathogens were isolated from cankered maize ear onto a standard medium. Fusarium colonies obtained were identified according to the Leisle taxonomic system after subculture and single-spore isolation. Of 271 isolates, 105 were identified as F. culmorum, contributing about 38.7% of the Fusarium isolates. The populations differed in different ecological areas, for example, F. culmorum was the dominant pathogen in Longdong and Longnan ecological areas, where its isolation frequencies were 52.0% and 55.4% respectively. The pathogenicity of F. culmorum was tested according to Koch’s Postulate using mixed isolates on maize varieties Shendan 16 and Jinsui 96832. F. culmorum was verified as pathogenic to maize ears. Three F. culmorum isolates were taken at random for sequences analysis of the rDNA-ITS gene. The PCR products of the three isolates were collected, purified, and sequenced. The sequences were aligned in GenBank. There was a very close relationship of isolates 3-1-1 with K1004 and K216, 6-4-1 and 21-2-1 with CBS122.73 downloaded from GenBank. Their maximum similarity was 99% with F. culmorum. Microsoft DNAStar was used to draw the phylogenetic tree of isolates 3-1-1, 6-4-1 and 21-2-1. This tree showed that 3-1-1 was in the same cluster as the K1004 while K216 isolates, 6-4-1 and 21-2-1 were in the same cluster as the CBS122.73 isolate, results that were consistent with morphological identification.

CLC Number:

S816
S513.01

GUO Cheng, WEI Hong-yu, GUO Man-ku, HE Su-qin, GUO Jian-guo, WANG Xiao-ming. Isolation, identification and analysis of rDNA-ITS gene sequence of Fusarium culmorum from maize ear rot samples[J]. Acta Prataculturae Sinica, 2013, 22(2): 171-177.

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