Abstract: To determine the safety of azoxystrobin in forages, residue degradation and final residue levels of azoxystrobin in forages and soils at three experimental sites (Beijing, Nanjing of Jiangsu province and Zhengzhou of Henan province) were determined by HPLC-MS. Residues of azoxystrobin in forage and soil were extracted by ethyl acetate, cleaned in a NH₂-column and detected by HPLC-MS. External standards were employed for quantitative analysis. The method was validated at three levels of fortification (0.01, 0.10 and 1.00 mg/kg) in forages and soils. The recoveries of azoxystrobin in forages were 86.5%-97.4%,with RSD of 6.1%-10.3%, and the recoveries in soils were 82.4%-107.2%, with RSD of 2.3%-5.7%. The limit of detection of azoxystrobin in forages and soils was 0.01 mg/kg. The half-lives of residues of azoxystrobin in forages at the three sites were 2.9-3.4 days,and the half-lives in soils were 3.2-3.7 days. Twenty one days after treatment, azoxystrobin residues in forages were safe according to the recommended dosage and times based on the CAC’s of azoxystrobin in forages (15 mg/kg).