Effect of long-term feeding of high concentrate diet on plasma biochemical parameters and expression of genes involved in glucose metabolism in lactating goats

doi:10.11686/cyxb20140535

Abstract

Abstract:

In order to investigate the effect of feeding high concentrate diets for a long-term on plasma biochemical parameters and expression of genes involved in glucose metabolism in different tissues, 8 healthy multiparous goats in mid-lactation were rumen-cannulated in and were randomly allocated to two groups, one receiving a diet with low concentrate (LC, concentrate∶roughage, %=40∶60) as the LC group (n=4), and another receiving high concentrate diet (HC, concentrate∶roughage, %=60∶40) as the HC group (n=4). The experiment lasted for 8 weeks. At the end of experiment, tissues including liver, muscle and fat were sampled and immediately immersed in liquid nitrogen and then stored at -70℃ for later gene expression analysis. After feeding the high-concentrate diet for 2 weeks, the mean ruminal pH value of HC group had declined to less than 5.8, with low pH persisting 3 hours after feeding. However, after being fed the high concentrate diet for 8 weeks, the HC goats showed an increase in ruminal pH (mean pH>6.0), although this value was still significantly lower than that of LC goats (P<0.05). Among the biochemical parameters detected in plasma, the level of glutamic oxalacetic transaminase (AST) showed a tendency to increase (P=0.09), and the level of glucose (P<0.05) and non-esterified fatty acid (NEFA, P=0.071) was increased in HC goats compare to control. The gene expression of Glut4, pyruvate carboxylase (PC) and mitochondrial type phosphoenolpyruvate carboxylase (PCK2) in liver, fat and muscle, respectively, showed a tendency to increase (P=0.06, P=0.066 and P=0.069, respectively). These results suggest that prolonged feeding of a high concentrate diet increased plasma NEFA, glucose and AST, and up-regulated gene expression involved in glucose metabolism.

CLC Number:

S827.4⁺2

DONG Hai-bo,WANG Shao-qing,JIA Yuan-yuan,DUANMU Yong-qian,TAO Shi-yu,NI Ying-dong,ZHANG Yuan-shu,ZHUANG Su,SHEN Xiang-zhen,ZHAO Ru-qian. Effect of long-term feeding of high concentrate diet on plasma biochemical parameters and expression of genes involved in glucose metabolism in lactating goats[J]. Acta Prataculturae Sinica, 2014, 23(5): 295-302.

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