Evaluation of physiological responses and tolerance to low-temperature stress of ten Elymus nutans varieties

doi:10.11686/cyxb2018357

Abstract

Abstract: This study investigated the cold-resistance capacity and adaptability of Elymus nutans germplasm collections from alpine grasslands. Germplasm collections of E. nutans were made from 7 localities in Gansu (Luqu, Zhuoni, Xiahe, Zhouqu, Hezuo, Lintan and Maqu), 2 localities in Sichuan (Aba and Ruoergai) and 1 locality in Qinghai Provinces (Gangcha). Plant seedlings (28 days old) from each collection were kept at 4 °C for 8, 24, and 72 h and physiological effects of the low temperature exposure were evaluated and the data analyzed using subordinate function analysis. It was found that under low temperature exposure, the relative membrane permeability of the germplasm collections was increased. The largest increases were found in Ruoergai and Aba for 8 h cold-stress (318% and 143%, respectively, compared to Control). Chlorophyll content was decreased by cold exposure, compared to Control, while free proline and soluble sugar were increased, with values of 458 μg·g^-1 FW and 4.80% FW in Aba and Zhuoni exceeding those of control by up to 95% and 207%, respectively. Enzyme activity of superoxide dismutase (SOD) and peroxidase (POD) was enhanced with short exposure, but weakened with longer exposure to cold stress. The strongest SOD enzymatic activity was found in Maqu and Luqu subjected to 24 h cold-stress (respectively, 147% and 148% higher than in Control plants). Meanwhile, the strongest POD enzymatic activity was found in Gangcha (220% higher than in Control plants). The evaluation of membership functions of the 10 regional germplasm collections on physiological index showed that those collected from Luqu, Zhuoni and Aba have stronger cold tolerance, the Ruoergai collection has moderate cold tolerance, and Lintan, Gangcha, Xiahe, Zhouqu, Hezuo and Maqu collections have weak cold tolerance.

Key words: Elymus nutans, cold-resistant capability, physiological index, subordinate function analysis

LIANG Kun-lun, JIA Cun-zhi, SUN Jin-hao, WANG Ming-yan, FU Hua, MAO Zhu-xin. Evaluation of physiological responses and tolerance to low-temperature stress of ten Elymus nutans varieties[J]. Acta Prataculturae Sinica, 2019, 28(3): 111-121.

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