Response of leaf osmolyte content to low temperature in autumn, and its relationship with chilling injury in centipede grass

doi:10.11686/cyxb2018218

Abstract

Abstract: Cold stress is a major problem for centipede grass (Eremochloa ophiuroides) and leaf osmolyte level is generally understood to be closely related to cold tolerance, but there are few studies specifically investigating response to cold stress in centipede grass. In this study, the relationship between cold tolerance of eight different genotypes and their leaf osmolyte content was studied in yield plot experiments conducted in Jurong Jiangsu during the winter chilling period from November to December of 2014. Our results indicated that the leaf color (greenness) was consistently decreased, while the leaf electrolyte leakage (EL) and malonaldehyde (MDA) contents were consistently increased during exposure to autumn chilling. Among the 8 genotypes, the EL and MDA content of genotypes E02, E07, E09 and E10 were significantly lower than those of the other four genotypes. However, the contents of soluble sugar, proline, spermidine and spermine of these genotypes were significantly higher than those of other genotypes. A correlation analysis of the data demonstrated a positive link between cold tolerance of centipede grass and particular osmolyte contents, in particular: soluble sugar, proline, spermidine, and spermine. Therefore, those four osmolytes in leaves play important roles in enhancing cold tolerance of centipede grass.

Key words: centipede grass, cold tolerance, soluble sugar, proline, polyamines

LIU Nan-qing, LIN Shao-yan, SHEN Yi-xin. Response of leaf osmolyte content to low temperature in autumn, and its relationship with chilling injury in centipede grass[J]. Acta Prataculturae Sinica, 2019, 28(3): 122-130.

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