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Acta Prataculturae Sinica ›› 2021, Vol. 30 ›› Issue (5): 75-83.DOI: 10.11686/cyxb2020505

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Effect of water stress on proline accumulation and metabolic pathways in Deschampsia caespitosa

Qiao-yu LUO1,2(), Yan-long WANG1, Zhi CHEN2, Yong-gui MA2, Qi-mei REN2, Yu-shou MA1()   

  1. 1.College of Agriculture and Animal Husbandry,Qinghai University,Xining 810008,China
    2.Qinghai Provincial Key Laboratory of Medicinal Plant and Animal Resources of Qinghai-Tibet Plateau,School of Life Sciences,Qinghai Normal University,Xining 810008,China
  • Received:2020-11-16 Revised:2021-01-07 Online:2021-05-20 Published:2021-04-16
  • Contact: Yu-shou MA

Abstract:

This research studied the accumulation of proline (Pro) and changes in the substrate, intermediate products and key enzymes in the Pro metabolic pathway in shoot and root tissues of Deschampsia caespitosa under drought and waterlogging stresses. It was found that: 1) Both drought and waterlogging stresses significantly increased the proline content of D. caespitosaP<0.05), and there was little difference between the proline content of the shoot and root under the same water treatment. 2) Under drought and waterlogging stresses, the glutamate content in the shoots and roots of D. caespitosa were significantly (P<0.05) decreased, but the glutamate concentration in the roots was greater than that in the shoots under the same water treatment. Water stress caused a significant decrease in ornithine content in the shoots (P<0.05), but not in roots. At the same time, the activities of Δ1-pyrroline-5-carboxylic acid synthetase, ornithine aminotransferase (δ-OAT) and Δ1-pyrroline-5-carboxylic acid reductase in the shoot and root tissues of D. caespitosa were significantly increased under drought and waterlogging stresses (P<0.05), and the activity of δ-OAT in shoots was stronger than in roots. In addition, the activities of Δ1-pyrroline-5-carboxylic acid dehydrogenase and proline dehydrogenase were significantly reduced (P<0.05). The results showed that the active accumulation of proline was a primary response of D. caespitosa in alleviation of drought and waterlogging stress. The glutamate and ornithine pathways operated synergetically in the shoots, but the glutamic acid pathway was the main pathway in the roots.

Key words: Deschampsia caespitosa, drought stress, waterlogging stress, proline, metabolic pathways