Welcome to Acta Prataculturae Sinica ! Today is Share:

Acta Prataculturae Sinica ›› 2022, Vol. 31 ›› Issue (3): 136-143.DOI: 10.11686/cyxb2020581

Previous Articles    

Effects of combined application of microbial fertilizer and chemical fertilizer on the growth of Lolium perenne

Dong-rong HAN1,2(), Tuo YAO1,2(), Hai-yun LI1,2, Shu-chao HUANG3, Yan-shan YANG1,2, Ya-min GAO1,2, Chang-ning LI1,2, Yin-cui ZHANG1,2   

  1. 1.Pratacultural College of Grassland Science,Gansu Agricultural University,Lanzhou 730070,China
    2.Key Laboratory of Grassland Ecosystem,Gansu Agricultural University,Ministry of Education,Lanzhou 730070,China
    3.College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China
  • Received:2020-12-24 Revised:2021-01-27 Online:2022-03-20 Published:2022-01-15
  • Contact: Tuo YAO


This experiment investigated the effect on perennial ryegrass (Lolium perenne) herbage yield in a pot experiment, of substituting 20% or 40% of a standard chemical fertilizer (CF) dressing with microbial fertilizer (MF). The CF applied to the control treatment was 300 kg·ha-1 of a proprietary fertilizer formulation with percentage nutrient analysis N∶P2O5∶K2O=14∶16∶15. MF was prepared by addition of bacterial cultures to a substrate of charcoal and crushed corn stalk and incubation for 7-10 days. The experimental treatments comprised a 20% or 40% reduction in CF (i.e. 240 or 180 kg·ha-1, respectively) and replacement with MF at 60, 90, 120, 150 or 180 kg·ha-1 making a total of ten CF-MF fertilizer combinations. Effects on agronomic traits including root morphology, nutritional quality and antioxidant enzyme activities of perennial ryegrass were measured. It was found that for the combination 80% CF with 90 kg·ha-1 MF, plant height, branch number and numbers of perennial ryegrass root tips, forks and link segments were increased(P<0.05) by 12.35%, 60.40% and 85.10%, respectively, compared with the control plants. For the treatment combination of 80% CF with 120 kg·ha-1 MF, ryegrass stem thickness, aboveground dry weight, underground dry weight, total root length, total root surface area, total root volume, root tip number, crude protein content and crude fat content were increased (P<0.05) by 29.41%, 16.84%, 45.54%, 21.15%, 14.59%, 166.67%, 47.35%, 5.71% and 37.98%, respectively, compared to the control plants. For the same treatment combination, shoot ascorbate peroxidase, super oxide dismutase, and peroxidase activities were decreased by 33.12%, 10.66% and 19.51% compared to control plants, while malondialdehyde concentration was 14.54% lower than in the control plants (P<0.05). MF applied at rates of 150 or 180 kg·ha-1 appeared to cause plant stress. The results indicate the optimal fertilizer combination to be a 20% reduction in chemical fertilizer and 90-120 kg·ha-1 microbial fertilizer.

Key words: perennial ryegrass, microbial fertilizer, agronomic traits, antioxidant enzyme activity, nutritional quality