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Acta Prataculturae Sinica ›› 2021, Vol. 30 ›› Issue (9): 97-104.DOI: 10.11686/cyxb2020338

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Effects of reduced glutathione on the growth and photosynthesis of perennial ryegrass under lead stress

Li-qing ZHAO1,2(), Xiang-yong PENG3, Jun-xiang LIU1, Jin-mei MAO4, Zhen-yuan SUN1()   

  1. 1.Key Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China
    2.The Open University of China,Beijing 100039,China
    3.College of Life Science Qufu Normal University,Qufu 273165,China
    4.Economic Forest Research Institute,Xinjiang Academy of Forestry,Urumqi 830063,China
  • Received:2020-07-15 Revised:2020-11-10 Online:2021-08-30 Published:2021-08-30
  • Contact: Zhen-yuan SUN

Abstract:

The aim of this study was to explore the mitigative effects of reduced glutathione (GSH) on the growth and photosynthesis of perennial ryegrass (Lolium perenne) under lead stress. Twelve-week old seedlings of the perennial ryegrass cultivar ‘cuttle’ were used as the experimental material. In the control (CK), leaves were sprayed with 50 mL distilled water, and roots were supplied with 1/2-strength modified Hoagland’s nutrient solution. In the other three experimental treatments, the roots were supplied with 1/2-strength modified Hoagland’s nutrient solution containing 0.75 mmol·L-1 Pb(NO32, and the leaves were sprayed with 50 mL distilled water (Pb treatment); with 25 mL distilled water followed by 25 mL 10 mmol·L-1 GSH (Pb+GSH treatment); or with 25 mL distilled water followed by 25 mL 1 mmol·L-1 L-buthionine-sulfoximine (BSO) (Pb+BSO treatment). The results showed that shoot length, root length, tiller number, biomass, chlorophyll content, photosynthetic parameters, and the kinetic parameters of chlorophyll fluorescence were significantly higher in the Pb+GSH treatment than in Pb treatment. In contrast, the values for root length, tiller number, biomass, carotenoid content, and photosynthetic parameters were lower in the Pb+BSO treatment than in Pb treatment. Together, our results show that spraying ryegrass leaves with 10 mmol·L-1 GSH can effectively alleviate the inhibitory effects of Pb stress and improve the Pb tolerance of perennial ryegrass, whereas spraying with BSO at 1 mmol·L-1 exacerbates the inhibitory effects of Pb stress.

Key words: perennial ryegrass, glutathione, growth, photosynthesis, lead