Acta Prataculturae Sinica ›› 2024, Vol. 33 ›› Issue (9): 169-184.DOI: 10.11686/cyxb2023381
Ming-ming GU(), Xing-hui JIANG, Zhi-yi MA, Shui-ling QIU, Hao-yu LIU, Ming-rui ZHANG, Jia-ning LU, Yu-jun QIU, Ben-zhi WANG, Qian-fu GAN()
Received:
2023-10-11
Revised:
2023-11-27
Online:
2024-09-20
Published:
2024-06-20
Contact:
Qian-fu GAN
Ming-ming GU, Xing-hui JIANG, Zhi-yi MA, Shui-ling QIU, Hao-yu LIU, Ming-rui ZHANG, Jia-ning LU, Yu-jun QIU, Ben-zhi WANG, Qian-fu GAN. Degradation characteristics of sweet potato and taro in the rumen of Mindong goats and changes in microbial community attached to the surface[J]. Acta Prataculturae Sinica, 2024, 33(9): 169-184.
项目Items | 含量Content (%) | 营养水平Nutrient levels2) | 含量Content |
---|---|---|---|
羊草Leymus chinensis | 55.00 | 干物质Dry matter (DM, %) | 88.69 |
玉米Corn | 31.00 | 代谢能Metabolic energy (ME, MJ·kg-1) | 11.67 |
豆粕Soybean meal | 12.20 | 粗蛋白质Crude protein (CP, %) | 10.94 |
食盐Salt | 0.50 | 中性洗涤纤维Neutral detergent fiber (NDF, %) | 48.66 |
石粉Limestone | 0.10 | 酸性洗涤纤维Acid detergent fiber (ADF, %) | 24.83 |
碳酸氢钙Ca(HCO3)2 | 0.20 | 粗脂肪Ether extract (EE, %) | 2.36 |
预混料Premix1) | 1.00 | 钙Calcium (Ca, %) | 0.62 |
合计Total | 100.00 | 磷Phosphorus (P, %) | 0.35 |
Table 1 Dietary composition and nutrient composition (dry matter basis)
项目Items | 含量Content (%) | 营养水平Nutrient levels2) | 含量Content |
---|---|---|---|
羊草Leymus chinensis | 55.00 | 干物质Dry matter (DM, %) | 88.69 |
玉米Corn | 31.00 | 代谢能Metabolic energy (ME, MJ·kg-1) | 11.67 |
豆粕Soybean meal | 12.20 | 粗蛋白质Crude protein (CP, %) | 10.94 |
食盐Salt | 0.50 | 中性洗涤纤维Neutral detergent fiber (NDF, %) | 48.66 |
石粉Limestone | 0.10 | 酸性洗涤纤维Acid detergent fiber (ADF, %) | 24.83 |
碳酸氢钙Ca(HCO3)2 | 0.20 | 粗脂肪Ether extract (EE, %) | 2.36 |
预混料Premix1) | 1.00 | 钙Calcium (Ca, %) | 0.62 |
合计Total | 100.00 | 磷Phosphorus (P, %) | 0.35 |
组别 Groups | 干物质 Dry matter | 粗蛋白 Crude protein | 淀粉 Starch |
---|---|---|---|
甘薯SP | 92.28±0.21a | 12.41±0.44a | 40.58±0.17b |
芋头TR | 94.39±0.39a | 10.09±0.21a | 62.47±0.22a |
Table 2 Nutrient content of sweet potato and taro (DM,%)
组别 Groups | 干物质 Dry matter | 粗蛋白 Crude protein | 淀粉 Starch |
---|---|---|---|
甘薯SP | 92.28±0.21a | 12.41±0.44a | 40.58±0.17b |
芋头TR | 94.39±0.39a | 10.09±0.21a | 62.47±0.22a |
项目 Items | 干物质瘤胃降解率 Rumen degradation rate of dry matter (%) | 粗蛋白瘤胃降解率 Rumen degradation rate of crude protein (%) | 淀粉瘤胃降解率 Rumen degradation rate of strach (%) | |||
---|---|---|---|---|---|---|
甘薯SP | 芋头TR | 甘薯SP | 芋头TR | 甘薯SP | 芋头TR | |
瘤胃滞留时间Rumen retention time (h) | ||||||
2 | 37.66±2.83Ae | 33.97±3.84Ae | 13.36±4.48Ae | 10.56±0.98Ae | 24.05±2.63Ae | 16.22±1.38Be |
4 | 54.79±2.28Ad | 46.52±6.45Ad | 23.84±3.45Ad | 25.91±3.19Ad | 38.91±4.10Ad | 24.83±3.33Bd |
8 | 59.35±1.02Ad | 54.87±6.67Ac | 31.42±1.75Ac | 28.09±2.39Acd | 51.84±3.39Ac | 39.39±2.05Bc |
12 | 69.16±0.63Ac | 68.77±2.11Ab | 35.82±2.53Ac | 31.89±1.84Ac | 55.22±3.20Ac | 42.40±1.30Bc |
24 | 84.54±7.46Ab | 82.45±2.69Aa | 45.52±1.48Ab | 44.44±0.96Ab | 70.27±1.81Ab | 58.10±1.14Bb |
36 | 88.79±1.06Aab | 84.87±1.60Ba | 49.07±1.00Aab | 47.55±0.87Aab | 73.47±1.43Aab | 69.85±1.32Ba |
48 | 91.43±1.08Aa | 88.31±1.32Ba | 52.22±1.63Aa | 50.43±1.10Aa | 77.41±1.89Aa | 72.56±2.77Ba |
时间Time | <0.05 | <0.05 | <0.05 | |||
样品Sample | <0.05 | <0.05 | <0.05 | |||
时间×样品 Time×sample | 0.69 | 0.35 | <0.05 | |||
瘤胃降解参数Rumen degradation parameters | ||||||
a (%) | 31.72±2.78A | 23.90±2.99B | 8.21±1.96A | 8.54±2.68A | 15.34±2.64A | 10.50±1.70B |
b (%) | 60.39±2.61B | 64.54±2.81A | 43.19±1.84A | 42.09±2.55A | 60.58±2.49B | 65.64±1.87A |
c (%·h-1) | 0.08±0.01A | 0.09±0.01A | 0.09±0.01A | 0.08±0.02A | 0.10±0.01A | 0.06±0.01A |
ED (%) | 66.48±4.28A | 62.88±6.41A | 34.28±4.22A | 32.62±6.75A | 53.44±5.62A | 43.60±5.39B |
Table 3 Rumen degradation properties of sweet potato and taro
项目 Items | 干物质瘤胃降解率 Rumen degradation rate of dry matter (%) | 粗蛋白瘤胃降解率 Rumen degradation rate of crude protein (%) | 淀粉瘤胃降解率 Rumen degradation rate of strach (%) | |||
---|---|---|---|---|---|---|
甘薯SP | 芋头TR | 甘薯SP | 芋头TR | 甘薯SP | 芋头TR | |
瘤胃滞留时间Rumen retention time (h) | ||||||
2 | 37.66±2.83Ae | 33.97±3.84Ae | 13.36±4.48Ae | 10.56±0.98Ae | 24.05±2.63Ae | 16.22±1.38Be |
4 | 54.79±2.28Ad | 46.52±6.45Ad | 23.84±3.45Ad | 25.91±3.19Ad | 38.91±4.10Ad | 24.83±3.33Bd |
8 | 59.35±1.02Ad | 54.87±6.67Ac | 31.42±1.75Ac | 28.09±2.39Acd | 51.84±3.39Ac | 39.39±2.05Bc |
12 | 69.16±0.63Ac | 68.77±2.11Ab | 35.82±2.53Ac | 31.89±1.84Ac | 55.22±3.20Ac | 42.40±1.30Bc |
24 | 84.54±7.46Ab | 82.45±2.69Aa | 45.52±1.48Ab | 44.44±0.96Ab | 70.27±1.81Ab | 58.10±1.14Bb |
36 | 88.79±1.06Aab | 84.87±1.60Ba | 49.07±1.00Aab | 47.55±0.87Aab | 73.47±1.43Aab | 69.85±1.32Ba |
48 | 91.43±1.08Aa | 88.31±1.32Ba | 52.22±1.63Aa | 50.43±1.10Aa | 77.41±1.89Aa | 72.56±2.77Ba |
时间Time | <0.05 | <0.05 | <0.05 | |||
样品Sample | <0.05 | <0.05 | <0.05 | |||
时间×样品 Time×sample | 0.69 | 0.35 | <0.05 | |||
瘤胃降解参数Rumen degradation parameters | ||||||
a (%) | 31.72±2.78A | 23.90±2.99B | 8.21±1.96A | 8.54±2.68A | 15.34±2.64A | 10.50±1.70B |
b (%) | 60.39±2.61B | 64.54±2.81A | 43.19±1.84A | 42.09±2.55A | 60.58±2.49B | 65.64±1.87A |
c (%·h-1) | 0.08±0.01A | 0.09±0.01A | 0.09±0.01A | 0.08±0.02A | 0.10±0.01A | 0.06±0.01A |
ED (%) | 66.48±4.28A | 62.88±6.41A | 34.28±4.22A | 32.62±6.75A | 53.44±5.62A | 43.60±5.39B |
指数 Index | 组别 Groups | 瘤胃滞留时间Rumen retention time (h) | ||||
---|---|---|---|---|---|---|
2 | 4 | 8 | 24 | 48 | ||
Chao1指数Chao1 index | 甘薯SP | 356.94±8.69 | 335.38±69.87 | 369.21±65.11 | 372.93±47.01 | 397.67±61.40 |
芋头TR | 347.52±140.01 | 336.43±77.29 | 391.02±64.27 | 418.41±53.97 | 393.52±80.38 | |
ACE指数ACE index | 甘薯SP | 351.87±8.02 | 335.86±68.88 | 369.74±65.84 | 370.46±46.49 | 395.04±62.42 |
芋头TR | 345.70±69.38 | 333.29±39.05 | 390.62±33.39 | 421.65±21.66 | 390.22±26.45 | |
Shannon指数Shannon index | 甘薯SP | 5.67±0.32 | 5.71±0.32 | 5.70±0.62 | 5.38±0.81 | 5.30±1.04 |
芋头TR | 5.77±0.43 | 5.60±0.37 | 6.05±0.32 | 6.30±0.17 | 5.34±0.17 | |
Simpson指数Simpson index | 甘薯SP | 0.94±0.02 | 0.94±0.01 | 0.92±0.03 | 0.89±0.09 | 0.87±0.11 |
芋头TR | 0.94±0.02 | 0.94±0.02 | 0.95±0.01 | 0.96±0.01 | 0.87±0.05 |
Table 4 Dynamic change of alpha diversity index of rumen bacteria attached to the surface of sweet potato and taro
指数 Index | 组别 Groups | 瘤胃滞留时间Rumen retention time (h) | ||||
---|---|---|---|---|---|---|
2 | 4 | 8 | 24 | 48 | ||
Chao1指数Chao1 index | 甘薯SP | 356.94±8.69 | 335.38±69.87 | 369.21±65.11 | 372.93±47.01 | 397.67±61.40 |
芋头TR | 347.52±140.01 | 336.43±77.29 | 391.02±64.27 | 418.41±53.97 | 393.52±80.38 | |
ACE指数ACE index | 甘薯SP | 351.87±8.02 | 335.86±68.88 | 369.74±65.84 | 370.46±46.49 | 395.04±62.42 |
芋头TR | 345.70±69.38 | 333.29±39.05 | 390.62±33.39 | 421.65±21.66 | 390.22±26.45 | |
Shannon指数Shannon index | 甘薯SP | 5.67±0.32 | 5.71±0.32 | 5.70±0.62 | 5.38±0.81 | 5.30±1.04 |
芋头TR | 5.77±0.43 | 5.60±0.37 | 6.05±0.32 | 6.30±0.17 | 5.34±0.17 | |
Simpson指数Simpson index | 甘薯SP | 0.94±0.02 | 0.94±0.01 | 0.92±0.03 | 0.89±0.09 | 0.87±0.11 |
芋头TR | 0.94±0.02 | 0.94±0.02 | 0.95±0.01 | 0.96±0.01 | 0.87±0.05 |
项目 Items | 组别 Groups | 瘤胃滞留时间Rumen retention time (h) | ||||
---|---|---|---|---|---|---|
2 | 4 | 8 | 24 | 48 | ||
拟杆菌门 Bacteroidota | 甘薯SP | 52.44±12.75ab | 53.62±6.33ab | 43.94±7.61b | 51.48±11.76ab | 62.63±13.12a |
芋头TR | 55.07±16.13 | 57.60±15.25 | 59.46±13.68 | 46.76±8.43 | 59.01±11.03 | |
变形菌门 Proteobacteria | 甘薯SP | 27.76±13.40 | 26.39±13.07 | 24.61±15.85 | 23.14±10.57 | 11.88±3.12 |
芋头TR | 27.45±24.17 | 27.22±21.22 | 21.31±18.30 | 26.25±12.19 | 12.74±8.67 | |
厚壁菌门 Firmicutes | 甘薯SP | 15.32±3.68 | 17.37±7.20 | 28.09±11.16 | 20.98±7.76 | 20.78±9.30 |
芋头TR | 16.20±8.03 | 13.07±6.37 | 14.62±4.87 | 23.09±4.72 | 22.70±5.77 | |
螺旋体门 Spirochaetes | 甘薯SP | 1.31±0.80 | 1.22±0.59 | 2.28±2.18 | 2.56±1.12 | 2.07±0.63 |
芋头TR | 0.35±0.17 | 0.81±0.67 | 2.07±1.24 | 2.56±1.21 | 3.76±2.08 | |
纤维杆菌门 Fibrobacterota | 甘薯SP | 2.89±2.76a | 1.15±1.05ab | 0.62±0.28ab | 1.02±0.76ab | 0.39±0.27b |
芋头TR | 0.61±0.06 | 0.91±0.45 | 0.89±0.49 | 0.39±0.20 | 0.74±0.56 | |
放线菌门 Actinobacteria | 甘薯SP | 0.90±0.51b | 0.11±0.08b | 0.28±0.16b | 0.60±0.33b | 2.09±1.95a |
芋头TR | 0.08±0.07b | 0.13±0.10b | 0.17±0.14b | 0.66±0.41a | 0.92±0.57a | |
脱硫杆菌门 Desulfobacterota | 甘薯SP | 0.11±0.01 | 0.10±0.05 | 0.14±0.12 | 0.13±0.11 | 0.13±0.02 |
芋头TR | 0.15±0.02 | 0.17±0.11 | 0.16±0.08 | 0.18±0.05 | 0.10±0.01 | |
迷踪菌门 Elusimicrobia | 甘薯SP | 0.42±0.03 | 0.00±0.01 | 0.03±0.01 | 0.04±0.03 | 0.01±0.00 |
芋头TR | 0.05±0.01 | 0.04±0.01 | 0.04±0.01 | 0.04±0.01 | 0.02±0.00 | |
其他菌门 Others | 甘薯SP | 0.02±0.01 | 0.01±0.00 | 0.00±0.00 | 0.03±0.01 | 0.00±0.00 |
芋头TR | 0.00±0.00 | 0.01±0.00 | 0.02±0.01 | 0.01±0.00 | 0.00±0.00 | |
疣微菌门 Verrucomicrobiota | 甘薯SP | 0.01±0.00 | 0.01±0.00 | 0.01±0.00 | 0.01±0.00 | 0.00±0.00 |
芋头TR | 0.02±0.00 | 0.04±0.01 | 0.04±0.01 | 0.03±0.02 | 0.00±0.00 |
Table 5 Dynamic changes of relative abundance of main bacteria phyla attached to the surface of sweet potato and taro (%)
项目 Items | 组别 Groups | 瘤胃滞留时间Rumen retention time (h) | ||||
---|---|---|---|---|---|---|
2 | 4 | 8 | 24 | 48 | ||
拟杆菌门 Bacteroidota | 甘薯SP | 52.44±12.75ab | 53.62±6.33ab | 43.94±7.61b | 51.48±11.76ab | 62.63±13.12a |
芋头TR | 55.07±16.13 | 57.60±15.25 | 59.46±13.68 | 46.76±8.43 | 59.01±11.03 | |
变形菌门 Proteobacteria | 甘薯SP | 27.76±13.40 | 26.39±13.07 | 24.61±15.85 | 23.14±10.57 | 11.88±3.12 |
芋头TR | 27.45±24.17 | 27.22±21.22 | 21.31±18.30 | 26.25±12.19 | 12.74±8.67 | |
厚壁菌门 Firmicutes | 甘薯SP | 15.32±3.68 | 17.37±7.20 | 28.09±11.16 | 20.98±7.76 | 20.78±9.30 |
芋头TR | 16.20±8.03 | 13.07±6.37 | 14.62±4.87 | 23.09±4.72 | 22.70±5.77 | |
螺旋体门 Spirochaetes | 甘薯SP | 1.31±0.80 | 1.22±0.59 | 2.28±2.18 | 2.56±1.12 | 2.07±0.63 |
芋头TR | 0.35±0.17 | 0.81±0.67 | 2.07±1.24 | 2.56±1.21 | 3.76±2.08 | |
纤维杆菌门 Fibrobacterota | 甘薯SP | 2.89±2.76a | 1.15±1.05ab | 0.62±0.28ab | 1.02±0.76ab | 0.39±0.27b |
芋头TR | 0.61±0.06 | 0.91±0.45 | 0.89±0.49 | 0.39±0.20 | 0.74±0.56 | |
放线菌门 Actinobacteria | 甘薯SP | 0.90±0.51b | 0.11±0.08b | 0.28±0.16b | 0.60±0.33b | 2.09±1.95a |
芋头TR | 0.08±0.07b | 0.13±0.10b | 0.17±0.14b | 0.66±0.41a | 0.92±0.57a | |
脱硫杆菌门 Desulfobacterota | 甘薯SP | 0.11±0.01 | 0.10±0.05 | 0.14±0.12 | 0.13±0.11 | 0.13±0.02 |
芋头TR | 0.15±0.02 | 0.17±0.11 | 0.16±0.08 | 0.18±0.05 | 0.10±0.01 | |
迷踪菌门 Elusimicrobia | 甘薯SP | 0.42±0.03 | 0.00±0.01 | 0.03±0.01 | 0.04±0.03 | 0.01±0.00 |
芋头TR | 0.05±0.01 | 0.04±0.01 | 0.04±0.01 | 0.04±0.01 | 0.02±0.00 | |
其他菌门 Others | 甘薯SP | 0.02±0.01 | 0.01±0.00 | 0.00±0.00 | 0.03±0.01 | 0.00±0.00 |
芋头TR | 0.00±0.00 | 0.01±0.00 | 0.02±0.01 | 0.01±0.00 | 0.00±0.00 | |
疣微菌门 Verrucomicrobiota | 甘薯SP | 0.01±0.00 | 0.01±0.00 | 0.01±0.00 | 0.01±0.00 | 0.00±0.00 |
芋头TR | 0.02±0.00 | 0.04±0.01 | 0.04±0.01 | 0.03±0.02 | 0.00±0.00 |
项目 Items | 组别 Groups | 瘤胃滞留时间Rumen retention time (h) | ||||
---|---|---|---|---|---|---|
2 | 4 | 8 | 24 | 48 | ||
普雷沃氏菌属Prevotella | 甘薯SP | 39.82±10.97 | 41.56±6.61 | 36.48±8.89 | 35.30±10.92 | 50.76±14.63 |
芋头TR | 36.52±9.87 | 40.94±9.35 | 41.79±9.08 | 29.89±5.76 | 46.83±17.85 | |
琥珀酸弧菌属Succinivibrio | 甘薯SP | 17.46±11.89a | 10.53±6.51ab | 12.48±8.78ab | 6.39±3.51ab | 3.67±2.64b |
芋头TR | 16.81±14.03 | 20.21±15.87 | 13.47±13.13 | 14.92±9.45 | 3.92±2.96 | |
瘤胃杆菌属Ruminobacter | 甘薯SP | 9.83±7.77 | 13.54±9.52 | 10.92±6.32 | 17.46±7.54 | 9.39±5.39 |
芋头TR | 10.34±5.17 | 6.65±4.68 | 7.45±6.92 | 10.82±6.31 | 8.52±7.47 | |
月形单胞菌属Selenomonas | 甘薯SP | 5.02±3.92 | 6.27±4.99 | 5.48±4.61 | 1.44±0.74 | 0.33±0.20 |
芋头TR | 1.72±0.99 | 0.90±0.48 | 0.62±0.55 | 1.15±0.63 | 0.45±0.05 | |
其他Others | 甘薯SP | 3.15±2.32 | 4.26±2.50 | 3.36±2.04 | 4.70±2.55 | 5.18±4.57 |
芋头TR | 1.05±0.50c | 0.95±0.38c | 1.90±0.83bc | 5.27±2.53ab | 8.21±5.08a | |
瘤胃球菌属Ruminococcus | 甘薯SP | 1.64±1.20 | 2.23±1.77 | 2.82±2.02 | 1.85±0.55 | 1.29±0.51 |
芋头TR | 1.90±0.21 | 1.37±1.26 | 2.17±2.06 | 6.59±5.63 | 5.14±3.60 | |
理研菌科_RC9肠群Rikenellaceae_RC9_gut_group | 甘薯SP | 2.27±0.74 | 2.05±0.63 | 2.00±0.72 | 3.07±1.87 | 2.31±2.00 |
芋头TR | 5.66±4.41 | 4.57±3.75 | 4.79±3.97 | 3.35±1.80 | 1.97±0.62 | |
F082 | 甘薯SP | 1.79±1.26 | 1.72±1.57 | 2.20±1.87 | 2.88±2.75 | 1.15±0.47 |
芋头TR | 4.19±2.48 | 4.24±3.45 | 3.33±3.22 | 1.98±1.47 | 0.90±0.21 | |
Muri菌属Muribaculaceae | 甘薯SP | 1.55±0.49 | 1.60±0.65 | 1.51±0.87 | 1.35±0.56 | 1.61±0.68 |
芋头TR | 1.65±0.93 | 1.64±0.16 | 2.06±0.76 | 2.69±1.13 | 3.33±2.02 | |
密螺旋体属Treponema | 甘薯SP | 1.30±0.80 | 1.22±0.72 | 1.62±0.20 | 2.54±1.11 | 2.51±1.03 |
芋头TR | 0.34±0.16 | 0.79±0.66 | 2.06±0.72 | 2.54±1.20 | 3.76±2.49 | |
乳杆菌属Lactobacillus | 甘薯SP | 0.36±0.19 | 0.49±0.07 | 8.01±7.49 | 0.67±0.53 | 0.47±0.40 |
芋头TR | 0.21±0.01 | 0.22±0.16 | 0.19±0.06 | 0.10±0.01 | 0.21±0.11 | |
沙特尔沃思氏菌属Shuttleworthia | 甘薯SP | 0.32±0.05 | 0.50±0.06 | 0.59±0.06 | 5.35±3.95 | 3.18±2.40 |
芋头TR | 0.02±0.01b | 0.03±0.01b | 0.11±0.10b | 0.74±0.37a | 0.71±0.57a | |
假丁酸弧菌属Pseudobutyrivibrio | 甘薯SP | 0.79±0.59Aa | 0.50±0.20Bab | 0.40±0.25Bab | 0.27±0.11Aab | 0.08±0.05Ab |
芋头TR | 1.15±0.27Aa | 1.45±0.56Aa | 1.51±0.42Aa | 0.51±0.30Ab | 0.16±0.09Ab | |
丝状杆菌属Fibrobacter | 甘薯SP | 2.89±1.28 | 1.14±1.09 | 0.53±0.30 | 1.01±0.76 | 0.48±0.28 |
芋头TR | 0.61±0.06 | 0.91±0.15 | 2.09±1.39 | 0.39±0.20 | 0.74±0.10 | |
普氏菌科UCG-003属Prevotellaceae_UCG-003 | 甘薯SP | 0.91±0.44 | 0.92±0.79 | 0.68±0.30 | 1.01±0.59 | 0.38±0.11 |
芋头TR | 2.47±1.55 | 1.86±0.47 | 2.04±0.97 | 1.63±0.89 | 1.05±0.77 |
Table 6 Dynamic changes of relative abundance of main bacteria genera attached to the surface of sweet potato and taro (%)
项目 Items | 组别 Groups | 瘤胃滞留时间Rumen retention time (h) | ||||
---|---|---|---|---|---|---|
2 | 4 | 8 | 24 | 48 | ||
普雷沃氏菌属Prevotella | 甘薯SP | 39.82±10.97 | 41.56±6.61 | 36.48±8.89 | 35.30±10.92 | 50.76±14.63 |
芋头TR | 36.52±9.87 | 40.94±9.35 | 41.79±9.08 | 29.89±5.76 | 46.83±17.85 | |
琥珀酸弧菌属Succinivibrio | 甘薯SP | 17.46±11.89a | 10.53±6.51ab | 12.48±8.78ab | 6.39±3.51ab | 3.67±2.64b |
芋头TR | 16.81±14.03 | 20.21±15.87 | 13.47±13.13 | 14.92±9.45 | 3.92±2.96 | |
瘤胃杆菌属Ruminobacter | 甘薯SP | 9.83±7.77 | 13.54±9.52 | 10.92±6.32 | 17.46±7.54 | 9.39±5.39 |
芋头TR | 10.34±5.17 | 6.65±4.68 | 7.45±6.92 | 10.82±6.31 | 8.52±7.47 | |
月形单胞菌属Selenomonas | 甘薯SP | 5.02±3.92 | 6.27±4.99 | 5.48±4.61 | 1.44±0.74 | 0.33±0.20 |
芋头TR | 1.72±0.99 | 0.90±0.48 | 0.62±0.55 | 1.15±0.63 | 0.45±0.05 | |
其他Others | 甘薯SP | 3.15±2.32 | 4.26±2.50 | 3.36±2.04 | 4.70±2.55 | 5.18±4.57 |
芋头TR | 1.05±0.50c | 0.95±0.38c | 1.90±0.83bc | 5.27±2.53ab | 8.21±5.08a | |
瘤胃球菌属Ruminococcus | 甘薯SP | 1.64±1.20 | 2.23±1.77 | 2.82±2.02 | 1.85±0.55 | 1.29±0.51 |
芋头TR | 1.90±0.21 | 1.37±1.26 | 2.17±2.06 | 6.59±5.63 | 5.14±3.60 | |
理研菌科_RC9肠群Rikenellaceae_RC9_gut_group | 甘薯SP | 2.27±0.74 | 2.05±0.63 | 2.00±0.72 | 3.07±1.87 | 2.31±2.00 |
芋头TR | 5.66±4.41 | 4.57±3.75 | 4.79±3.97 | 3.35±1.80 | 1.97±0.62 | |
F082 | 甘薯SP | 1.79±1.26 | 1.72±1.57 | 2.20±1.87 | 2.88±2.75 | 1.15±0.47 |
芋头TR | 4.19±2.48 | 4.24±3.45 | 3.33±3.22 | 1.98±1.47 | 0.90±0.21 | |
Muri菌属Muribaculaceae | 甘薯SP | 1.55±0.49 | 1.60±0.65 | 1.51±0.87 | 1.35±0.56 | 1.61±0.68 |
芋头TR | 1.65±0.93 | 1.64±0.16 | 2.06±0.76 | 2.69±1.13 | 3.33±2.02 | |
密螺旋体属Treponema | 甘薯SP | 1.30±0.80 | 1.22±0.72 | 1.62±0.20 | 2.54±1.11 | 2.51±1.03 |
芋头TR | 0.34±0.16 | 0.79±0.66 | 2.06±0.72 | 2.54±1.20 | 3.76±2.49 | |
乳杆菌属Lactobacillus | 甘薯SP | 0.36±0.19 | 0.49±0.07 | 8.01±7.49 | 0.67±0.53 | 0.47±0.40 |
芋头TR | 0.21±0.01 | 0.22±0.16 | 0.19±0.06 | 0.10±0.01 | 0.21±0.11 | |
沙特尔沃思氏菌属Shuttleworthia | 甘薯SP | 0.32±0.05 | 0.50±0.06 | 0.59±0.06 | 5.35±3.95 | 3.18±2.40 |
芋头TR | 0.02±0.01b | 0.03±0.01b | 0.11±0.10b | 0.74±0.37a | 0.71±0.57a | |
假丁酸弧菌属Pseudobutyrivibrio | 甘薯SP | 0.79±0.59Aa | 0.50±0.20Bab | 0.40±0.25Bab | 0.27±0.11Aab | 0.08±0.05Ab |
芋头TR | 1.15±0.27Aa | 1.45±0.56Aa | 1.51±0.42Aa | 0.51±0.30Ab | 0.16±0.09Ab | |
丝状杆菌属Fibrobacter | 甘薯SP | 2.89±1.28 | 1.14±1.09 | 0.53±0.30 | 1.01±0.76 | 0.48±0.28 |
芋头TR | 0.61±0.06 | 0.91±0.15 | 2.09±1.39 | 0.39±0.20 | 0.74±0.10 | |
普氏菌科UCG-003属Prevotellaceae_UCG-003 | 甘薯SP | 0.91±0.44 | 0.92±0.79 | 0.68±0.30 | 1.01±0.59 | 0.38±0.11 |
芋头TR | 2.47±1.55 | 1.86±0.47 | 2.04±0.97 | 1.63±0.89 | 1.05±0.77 |
项目 Items | 组别 Groups | 瘤胃滞留时间Rumen retention time (h) | ||||
---|---|---|---|---|---|---|
2 | 4 | 8 | 24 | 48 | ||
氨基糖和核苷酸糖代谢Amino sugar and nucleotide sugar metabolism | 甘薯SP | 1.31±0.07 | 1.32±0.09 | 1.32±0.13 | 1.27±0.07 | 1.28±0.03 |
芋头TR | 1.29±0.05 | 1.31±0.01 | 1.32±0.04 | 1.27±0.04 | 1.26±0.02 | |
丙酮酸代谢Pyruvate metabolism | 甘薯SP | 0.92±0.05 | 0.93±0.08 | 0.96±0.05 | 0.96±0.03 | 0.93±0.03 |
芋头TR | 0.95±0.03 | 0.93±0.01 | 0.92±0.03 | 0.96±0.04 | 0.93±0.04 | |
淀粉和蔗糖代谢Starch and sucrose metabolism | 甘薯SP | 0.98±0.11 | 1.03±0.16 | 1.09±0.27 | 1.02±0.08 | 1.09±0.04 |
芋头TR | 0.95±0.08b | 0.95±0.04b | 0.99±0.05b | 1.00±0.03ab | 1.08±0.07a | |
磷酸戊糖途径Pentose phosphate pathway | 甘薯SP | 0.68±0.02b | 0.68±0.01b | 0.71±0.03b | 0.70±0.01b | 0.74±0.01a |
芋头TR | 0.69±0.01b | 0.69±0.01b | 0.69±0.01b | 0.70±0.01ab | 0.72±0.02a | |
乙醛酸和二羧酸代谢Glyoxylate and dicarboxylate metabolism | 甘薯SP | 0.74±0.03a | 0.75±0.03a | 0.72±0.03a | 0.72±0.03a | 0.64±0.02b |
芋头TR | 0.74±0.04a | 0.74±0.04a | 0.75±0.04a | 0.72±0.02ab | 0.66±0.05b | |
果糖和甘露糖代谢Fructose and mannose metabolism | 甘薯SP | 0.71±0.09 | 0.73±0.14 | 0.74±0.15 | 0.67±0.08 | 0.74±0.05 |
芋头TR | 0.68±0.11 | 0.69±0.08 | 0.72±0.08 | 0.66±0.06 | 0.72±0.06 | |
丁酸代谢Butanoate metabolism | 甘薯SP | 0.59±0.02 | 0.59±0.04 | 0.59±0.03 | 0.57±0.01 | 0.57±0.01 |
芋头TR | 0.58±0.02 | 0.58±0.02 | 0.58±0.02 | 0.58±0.02 | 0.57±0.02 | |
半乳糖代谢Galactose metabolism | 甘薯SP | 0.71±0.07 | 0.74±0.10 | 0.74±0.11 | 0.72±0.06 | 0.73±0.02 |
芋头TR | 0.70±0.06 | 0.72±0.04 | 0.73±0.05 | 0.70±0.03 | 0.74±0.03 | |
丙酸代谢Propanoate metabolism | 甘薯SP | 0.45±0.03ab | 0.46±0.03ab | 0.46±0.04ab | 0.48±0.03a | 0.40±0.06b |
芋头TR | 0.48±0.04ab | 0.46±0.03ab | 0.45±0.04ab | 0.49±0.02a | 0.41±0.05b | |
C5-支链二元酸代谢C5-branched dibasic acid metabolism | 甘薯SP | 0.26±0.02 | 0.26±0.01 | 0.24±0.05 | 0.26±0.02 | 0.21±0.03 |
芋头TR | 0.26±0.05 | 0.26±0.05 | 0.26±0.04 | 0.28±0.03 | 0.23±0.04 |
Table 7 Changes in carbohydrate metabolic pathway abundance at KEGG3 level (%)
项目 Items | 组别 Groups | 瘤胃滞留时间Rumen retention time (h) | ||||
---|---|---|---|---|---|---|
2 | 4 | 8 | 24 | 48 | ||
氨基糖和核苷酸糖代谢Amino sugar and nucleotide sugar metabolism | 甘薯SP | 1.31±0.07 | 1.32±0.09 | 1.32±0.13 | 1.27±0.07 | 1.28±0.03 |
芋头TR | 1.29±0.05 | 1.31±0.01 | 1.32±0.04 | 1.27±0.04 | 1.26±0.02 | |
丙酮酸代谢Pyruvate metabolism | 甘薯SP | 0.92±0.05 | 0.93±0.08 | 0.96±0.05 | 0.96±0.03 | 0.93±0.03 |
芋头TR | 0.95±0.03 | 0.93±0.01 | 0.92±0.03 | 0.96±0.04 | 0.93±0.04 | |
淀粉和蔗糖代谢Starch and sucrose metabolism | 甘薯SP | 0.98±0.11 | 1.03±0.16 | 1.09±0.27 | 1.02±0.08 | 1.09±0.04 |
芋头TR | 0.95±0.08b | 0.95±0.04b | 0.99±0.05b | 1.00±0.03ab | 1.08±0.07a | |
磷酸戊糖途径Pentose phosphate pathway | 甘薯SP | 0.68±0.02b | 0.68±0.01b | 0.71±0.03b | 0.70±0.01b | 0.74±0.01a |
芋头TR | 0.69±0.01b | 0.69±0.01b | 0.69±0.01b | 0.70±0.01ab | 0.72±0.02a | |
乙醛酸和二羧酸代谢Glyoxylate and dicarboxylate metabolism | 甘薯SP | 0.74±0.03a | 0.75±0.03a | 0.72±0.03a | 0.72±0.03a | 0.64±0.02b |
芋头TR | 0.74±0.04a | 0.74±0.04a | 0.75±0.04a | 0.72±0.02ab | 0.66±0.05b | |
果糖和甘露糖代谢Fructose and mannose metabolism | 甘薯SP | 0.71±0.09 | 0.73±0.14 | 0.74±0.15 | 0.67±0.08 | 0.74±0.05 |
芋头TR | 0.68±0.11 | 0.69±0.08 | 0.72±0.08 | 0.66±0.06 | 0.72±0.06 | |
丁酸代谢Butanoate metabolism | 甘薯SP | 0.59±0.02 | 0.59±0.04 | 0.59±0.03 | 0.57±0.01 | 0.57±0.01 |
芋头TR | 0.58±0.02 | 0.58±0.02 | 0.58±0.02 | 0.58±0.02 | 0.57±0.02 | |
半乳糖代谢Galactose metabolism | 甘薯SP | 0.71±0.07 | 0.74±0.10 | 0.74±0.11 | 0.72±0.06 | 0.73±0.02 |
芋头TR | 0.70±0.06 | 0.72±0.04 | 0.73±0.05 | 0.70±0.03 | 0.74±0.03 | |
丙酸代谢Propanoate metabolism | 甘薯SP | 0.45±0.03ab | 0.46±0.03ab | 0.46±0.04ab | 0.48±0.03a | 0.40±0.06b |
芋头TR | 0.48±0.04ab | 0.46±0.03ab | 0.45±0.04ab | 0.49±0.02a | 0.41±0.05b | |
C5-支链二元酸代谢C5-branched dibasic acid metabolism | 甘薯SP | 0.26±0.02 | 0.26±0.01 | 0.24±0.05 | 0.26±0.02 | 0.21±0.03 |
芋头TR | 0.26±0.05 | 0.26±0.05 | 0.26±0.04 | 0.28±0.03 | 0.23±0.04 |
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